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Electrochemical genosensors for biomedical applications based on gold nanoparticles

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Title: Electrochemical genosensors for biomedical applications based on gold nanoparticles
Authors: Castañeda, M.T., Merkoçi, A. arben.merkoci@uab.es, Pumera, M.1, Alegret, S.1
Source: Biosensors & Bioelectronics. Apr2007, Vol. 22 Issue 9/10, p1961-1967. 7p.
Subjects: Biosensors, Electrochemical sensors, Nanoparticles, Gold
Abstract: Abstract: Two gold nanoparticles-based genomagnetic sensors designs for detection of DNA hybridization are described. Both assays are based on a magnetically induced direct electrochemical detection of gold tags on magnetic graphite-epoxy composite electrodes. The first design is a two strands assay format that consists of the hybridization between a capture DNA strand which is linked with paramagnetic beads and another DNA strand related to BRCA1 breast cancer gene used as a target which is coupled with streptavidin-gold nanoparticles. The second genomagnetic sensor design is a sandwich assay format with more application possibilities. A cystic fibrosis related DNA strand is used as a target and sandwiched between two complementary DNA probes: the first one linked with paramagnetic beads and a second one modified with gold nanoparticles via biotin–streptavidin complexation reactions. The electrochemical detection of gold nanoparticles by differential pulse voltammetry was performed in both cases. The developed genomagnetic sensors provide a reliable discrimination against noncomplementary DNA as well against one and three-base mismatches. Optimization parameters affecting the hybridization and analytical performance of the developed genosensors are shown for genomagnetic assays of DNA sequences related with the breast cancer and cystic fibrosis genes. [Copyright &y& Elsevier]
Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 24300627
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Electrochemical genosensors for biomedical applications based on gold nanoparticles
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  Data: <searchLink fieldCode="AR" term="%22Castañeda%2C+M%2ET%2E%22">Castañeda, M.T.</searchLink><br /><searchLink fieldCode="AR" term="%22Merkoçi%2C+A%2E%22">Merkoçi, A.</searchLink><i> arben.merkoci@uab.es</i><br /><searchLink fieldCode="AR" term="%22Pumera%2C+M%2E%22">Pumera, M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Alegret%2C+S%2E%22">Alegret, S.</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Biosensors+%26+Bioelectronics%22">Biosensors & Bioelectronics</searchLink>. Apr2007, Vol. 22 Issue 9/10, p1961-1967. 7p.
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  Label: Abstract
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  Data: Abstract: Two gold nanoparticles-based genomagnetic sensors designs for detection of DNA hybridization are described. Both assays are based on a magnetically induced direct electrochemical detection of gold tags on magnetic graphite-epoxy composite electrodes. The first design is a two strands assay format that consists of the hybridization between a capture DNA strand which is linked with paramagnetic beads and another DNA strand related to BRCA1 breast cancer gene used as a target which is coupled with streptavidin-gold nanoparticles. The second genomagnetic sensor design is a sandwich assay format with more application possibilities. A cystic fibrosis related DNA strand is used as a target and sandwiched between two complementary DNA probes: the first one linked with paramagnetic beads and a second one modified with gold nanoparticles via biotin–streptavidin complexation reactions. The electrochemical detection of gold nanoparticles by differential pulse voltammetry was performed in both cases. The developed genomagnetic sensors provide a reliable discrimination against noncomplementary DNA as well against one and three-base mismatches. Optimization parameters affecting the hybridization and analytical performance of the developed genosensors are shown for genomagnetic assays of DNA sequences related with the breast cancer and cystic fibrosis genes. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.bios.2006.08.031
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        Text: English
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        StartPage: 1961
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        Type: general
      – SubjectFull: Electrochemical sensors
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      – SubjectFull: Nanoparticles
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              Text: Apr2007
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              Y: 2007
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