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Stochastic Sensing on a Modular Chip Containing a Single-Ion Channel.

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Title: Stochastic Sensing on a Modular Chip Containing a Single-Ion Channel.
Authors: Ji Wook Shim1, Li Qun Gu1 gul@missouri.edu
Source: Analytical Chemistry. 3/15/2007, Vol. 79 Issue 6, p2207-2213. 7p. 1 Diagram, 1 Chart, 1 Graph.
Subjects: Protein research, Modular construction, Biochemic medicine, Medical sciences, Binding sites, Bioindicators, Genetic markers, Chemistry, Biology, Biochemistry
Abstract: Engineered protein channels have many potential applications in biosensing at the single-molecule level. A future generation of biosensor could be an array of target-specific ion channels, where each protein pore acts as a sensor element. An important step toward this goal is to create a portable, durable, single-protein channel-integrated chip device. Here we report a versatile, modular chip that contains a single-ion channel for single-molecular bio-sensing. The core of the device is a long-lived lipid membrane that has been sandwiched between two air-insulated agarose layers which gel in situ. A single-protein pore embedded in the membrane serves as the sensor element. The modular device is highly portable, allowing a single-ion channel to continuously function following detachment of the chip from the instrument and independent transportation of the device. The chip also exhibits high durability, which is evidenced from long-duration continuous observation of single-channel dynamics. Once engineered protein pores are installed, the chip becomes a robust stochastic sensor for real-time targeting such as detection of the second messenger IP3. This pluggable biochip could be incorporated with many applicable devices, such as a microfluidic system, and be made into a microarray for both biomedical detection and membrane protein research. [ABSTRACT FROM AUTHOR]
Copyright of Analytical Chemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubType: Academic Journal
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  Data: Stochastic Sensing on a Modular Chip Containing a Single-Ion Channel.
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  Data: <searchLink fieldCode="AR" term="%22Ji+Wook+Shim%22">Ji Wook Shim</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Li+Qun+Gu%22">Li Qun Gu</searchLink><relatesTo>1</relatesTo><i> gul@missouri.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Analytical+Chemistry%22">Analytical Chemistry</searchLink>. 3/15/2007, Vol. 79 Issue 6, p2207-2213. 7p. 1 Diagram, 1 Chart, 1 Graph.
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  Data: <searchLink fieldCode="DE" term="%22Protein+research%22">Protein research</searchLink><br /><searchLink fieldCode="DE" term="%22Modular+construction%22">Modular construction</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemic+medicine%22">Biochemic medicine</searchLink><br /><searchLink fieldCode="DE" term="%22Medical+sciences%22">Medical sciences</searchLink><br /><searchLink fieldCode="DE" term="%22Binding+sites%22">Binding sites</searchLink><br /><searchLink fieldCode="DE" term="%22Bioindicators%22">Bioindicators</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+markers%22">Genetic markers</searchLink><br /><searchLink fieldCode="DE" term="%22Chemistry%22">Chemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Biology%22">Biology</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink>
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  Label: Abstract
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  Data: Engineered protein channels have many potential applications in biosensing at the single-molecule level. A future generation of biosensor could be an array of target-specific ion channels, where each protein pore acts as a sensor element. An important step toward this goal is to create a portable, durable, single-protein channel-integrated chip device. Here we report a versatile, modular chip that contains a single-ion channel for single-molecular bio-sensing. The core of the device is a long-lived lipid membrane that has been sandwiched between two air-insulated agarose layers which gel in situ. A single-protein pore embedded in the membrane serves as the sensor element. The modular device is highly portable, allowing a single-ion channel to continuously function following detachment of the chip from the instrument and independent transportation of the device. The chip also exhibits high durability, which is evidenced from long-duration continuous observation of single-channel dynamics. Once engineered protein pores are installed, the chip becomes a robust stochastic sensor for real-time targeting such as detection of the second messenger IP3. This pluggable biochip could be incorporated with many applicable devices, such as a microfluidic system, and be made into a microarray for both biomedical detection and membrane protein research. [ABSTRACT FROM AUTHOR]
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  Label:
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  Data: <i>Copyright of Analytical Chemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1021/ac0614285
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      – Code: eng
        Text: English
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        PageCount: 7
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      – SubjectFull: Protein research
        Type: general
      – SubjectFull: Modular construction
        Type: general
      – SubjectFull: Biochemic medicine
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      – SubjectFull: Medical sciences
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      – SubjectFull: Binding sites
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      – SubjectFull: Bioindicators
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      – SubjectFull: Genetic markers
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      – SubjectFull: Chemistry
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      – SubjectFull: Biology
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      – SubjectFull: Biochemistry
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      – TitleFull: Stochastic Sensing on a Modular Chip Containing a Single-Ion Channel.
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            NameFull: Ji Wook Shim
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            NameFull: Li Qun Gu
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            – D: 15
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              Text: 3/15/2007
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              Y: 2007
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              Value: 79
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              Value: 6
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