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Isolation of lactoferrin from bovine colostrum by ultrafiltration coupled with strong cation exchange chromatography on a production scale

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Title: Isolation of lactoferrin from bovine colostrum by ultrafiltration coupled with strong cation exchange chromatography on a production scale
Authors: Lu, Rong Rong lurr@sytu.edu.cn, Xu, Shi Ying1, Wang, Zhang1, Yang, Rui Jin1
Source: Journal of Membrane Science. Jul2007, Vol. 297 Issue 1/2, p152-161. 10p.
Subjects: Lactoferrin, Colostrum, Cations, Chromatographic analysis
Abstract: Abstract: Lactoferrin (LF) was isolated from bovine colostrum by ultrafiltration and then purified with a fast flow strong cation exchange chromatography system in a production scale. A two-step ultrafiltration process was performed with membranes of nominal molecular weight cut-offs of 100kDa for ultrafiltration (UF) step 1 (UF-1) and 10kDa in the UF step 2 (UF-2). The UF-1 process was performed at fixed transmembrane pressure (TMP), tangential flow velocity and temperature equivalent to 200kPa, 5m/s, and 25°C, respectively. The optimum operating parameters for the UF-2 were a tangential flow velocity of 4m/s, limiting TMP of 150kPa, and an operating temperature of 50°C. The predicted permeate flux based on a resistance mathematical model was not significantly (p >0.05) different from those of the actual experiment. The LF concentrated in the UF-2 retentate reached a purity of 30.88% (w/w) and a recovery of 94.04%. A stepwise procedure for purification of the crude LF was conducted using a preparative-scale strong cation exchange chromatography. The LF eluted with 1.0M NaCl aqueous buffer showed a single band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a molecular weight of 80,400Da. The purity and the recovery of the final LF product were 94.20% and 82.46%, respectively. The process developed in this work is a significant improvement over the commercial practice for the fractionation of LF from bovine colostrum. [Copyright &y& Elsevier]
Copyright of Journal of Membrane Science is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 25105945
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  Label: Title
  Group: Ti
  Data: Isolation of lactoferrin from bovine colostrum by ultrafiltration coupled with strong cation exchange chromatography on a production scale
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  Data: <searchLink fieldCode="AR" term="%22Lu%2C+Rong+Rong%22">Lu, Rong Rong</searchLink><i> lurr@sytu.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Xu%2C+Shi+Ying%22">Xu, Shi Ying</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Zhang%22">Wang, Zhang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yang%2C+Rui+Jin%22">Yang, Rui Jin</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Membrane+Science%22">Journal of Membrane Science</searchLink>. Jul2007, Vol. 297 Issue 1/2, p152-161. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Lactoferrin%22">Lactoferrin</searchLink><br /><searchLink fieldCode="DE" term="%22Colostrum%22">Colostrum</searchLink><br /><searchLink fieldCode="DE" term="%22Cations%22">Cations</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Lactoferrin (LF) was isolated from bovine colostrum by ultrafiltration and then purified with a fast flow strong cation exchange chromatography system in a production scale. A two-step ultrafiltration process was performed with membranes of nominal molecular weight cut-offs of 100kDa for ultrafiltration (UF) step 1 (UF-1) and 10kDa in the UF step 2 (UF-2). The UF-1 process was performed at fixed transmembrane pressure (TMP), tangential flow velocity and temperature equivalent to 200kPa, 5m/s, and 25°C, respectively. The optimum operating parameters for the UF-2 were a tangential flow velocity of 4m/s, limiting TMP of 150kPa, and an operating temperature of 50°C. The predicted permeate flux based on a resistance mathematical model was not significantly (p >0.05) different from those of the actual experiment. The LF concentrated in the UF-2 retentate reached a purity of 30.88% (w/w) and a recovery of 94.04%. A stepwise procedure for purification of the crude LF was conducted using a preparative-scale strong cation exchange chromatography. The LF eluted with 1.0M NaCl aqueous buffer showed a single band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with a molecular weight of 80,400Da. The purity and the recovery of the final LF product were 94.20% and 82.46%, respectively. The process developed in this work is a significant improvement over the commercial practice for the fractionation of LF from bovine colostrum. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Membrane Science is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.memsci.2007.03.039
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      – Code: eng
        Text: English
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        PageCount: 10
        StartPage: 152
    Subjects:
      – SubjectFull: Lactoferrin
        Type: general
      – SubjectFull: Colostrum
        Type: general
      – SubjectFull: Cations
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      – SubjectFull: Chromatographic analysis
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      – TitleFull: Isolation of lactoferrin from bovine colostrum by ultrafiltration coupled with strong cation exchange chromatography on a production scale
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            NameFull: Lu, Rong Rong
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            NameFull: Xu, Shi Ying
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            NameFull: Wang, Zhang
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            NameFull: Yang, Rui Jin
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            – D: 05
              M: 07
              Text: Jul2007
              Type: published
              Y: 2007
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              Value: 297
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