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Enhancing the Sensitivity of Assays to Detect T Cell Reactivity: The Effect of Cell Separation and Cryopreservation Media.

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Title: Enhancing the Sensitivity of Assays to Detect T Cell Reactivity: The Effect of Cell Separation and Cryopreservation Media.
Authors: TREE, TIMOTHY I.M.1 (AUTHOR), ROEP, BART O.2 (AUTHOR), PEAKMAN, MARK1 (AUTHOR)
Source: Annals of the New York Academy of Sciences. 2004, Vol. 1037 Issue 1, p26-32. 7p.
Subjects: Cryopreservation of organs, tissues, etc., Diabetes, T cells, Diabetic acidosis, Cell separation
Abstract: The identification of sensitive assay formats capable of distinguishing islet autoreactive T cells directly ex vivo in blood is a major goal in type 1 diabetes research. Whereas much effort has been made to identify suitable assay formats, relatively little attention has been paid to optimizing the quality of cell preparations used. To address this issue we investigated the role of two key variables in the preparation of peripheral blood mononuclear cells (PBMCs): the separation media used in density gradient centrifugation and the method of cryopreservation. PBMCs were prepared from a single individual using different protocols and tested for responses to suboptimal concentrations of tetanus toxoid, representing a low frequency recall response. Significant differences were observed in T cell responses dependent upon the selection of the separation media and cryopreservation methods used, indicating that relatively small differences in preparation of PBMCs can have measurable effects on assay sensitivity. [ABSTRACT FROM AUTHOR]
Copyright of Annals of the New York Academy of Sciences is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 25195380
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Enhancing the Sensitivity of Assays to Detect T Cell Reactivity: The Effect of Cell Separation and Cryopreservation Media.
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  Data: <searchLink fieldCode="JN" term="%22Annals+of+the+New+York+Academy+of+Sciences%22">Annals of the New York Academy of Sciences</searchLink>. 2004, Vol. 1037 Issue 1, p26-32. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Cryopreservation+of+organs%2C+tissues%2C+etc%2E%22">Cryopreservation of organs, tissues, etc.</searchLink><br /><searchLink fieldCode="DE" term="%22Diabetes%22">Diabetes</searchLink><br /><searchLink fieldCode="DE" term="%22T+cells%22">T cells</searchLink><br /><searchLink fieldCode="DE" term="%22Diabetic+acidosis%22">Diabetic acidosis</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+separation%22">Cell separation</searchLink>
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  Data: The identification of sensitive assay formats capable of distinguishing islet autoreactive T cells directly ex vivo in blood is a major goal in type 1 diabetes research. Whereas much effort has been made to identify suitable assay formats, relatively little attention has been paid to optimizing the quality of cell preparations used. To address this issue we investigated the role of two key variables in the preparation of peripheral blood mononuclear cells (PBMCs): the separation media used in density gradient centrifugation and the method of cryopreservation. PBMCs were prepared from a single individual using different protocols and tested for responses to suboptimal concentrations of tetanus toxoid, representing a low frequency recall response. Significant differences were observed in T cell responses dependent upon the selection of the separation media and cryopreservation methods used, indicating that relatively small differences in preparation of PBMCs can have measurable effects on assay sensitivity. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Annals of the New York Academy of Sciences is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1196/annals.1337.005
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      – Code: eng
        Text: English
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        PageCount: 7
        StartPage: 26
    Subjects:
      – SubjectFull: Cryopreservation of organs, tissues, etc.
        Type: general
      – SubjectFull: Diabetes
        Type: general
      – SubjectFull: T cells
        Type: general
      – SubjectFull: Diabetic acidosis
        Type: general
      – SubjectFull: Cell separation
        Type: general
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      – TitleFull: Enhancing the Sensitivity of Assays to Detect T Cell Reactivity: The Effect of Cell Separation and Cryopreservation Media.
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              M: 12
              Text: 2004
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              Value: 1037
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