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Phosphopeptide analysis by directly coupling two-dimensional planar electrochromatography/thin-layer chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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Title: Phosphopeptide analysis by directly coupling two-dimensional planar electrochromatography/thin-layer chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Authors: Panchagnula, Venkateswarlu1, Mikulskis, Alvydas1, Song, Linan1, Wang, Yang1, Wang, Mei1, Knubovets, Tanya1, Scrivener, Elaine2, Golenko, Eva1, Krull, Ira S.3, Schulz, Michael4, Heinz-Emil-Hauck4, Patton, Wayne F.1 wayne.patton@perkinelmer.com
Source: Journal of Chromatography A. Jun2007, Vol. 1155 Issue 1, p112-123. 12p.
Subjects: Chromatographic analysis, Peptides, Proteins, Mass spectrometry
Abstract: Abstract: A novel strategy is presented for the fractionation of complex peptide mixtures using two-dimensional planar electrochromatography/thin-layer chromatography (2D PEC/TLC). Phosphopeptides migrate more slowly in the first dimension, based upon their anionic phosphate residues, and certain predominantly acidic phosphopeptides even migrate in the opposite direction, relative to the bulk of the peptides. Phosphopeptides are further distinguished based upon hydrophilicity in the second dimension. This permits a restricted region of the plate to be directly interrogated for the presence of phosphopeptides by mass spectrometry (MS). Phosphopeptide analysis from the plates by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)-MS and tandem MS enabled peptide sequencing and identification. [Copyright &y& Elsevier]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 25320685
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Phosphopeptide analysis by directly coupling two-dimensional planar electrochromatography/thin-layer chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
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  Data: <searchLink fieldCode="AR" term="%22Panchagnula%2C+Venkateswarlu%22">Panchagnula, Venkateswarlu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Mikulskis%2C+Alvydas%22">Mikulskis, Alvydas</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Song%2C+Linan%22">Song, Linan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Yang%22">Wang, Yang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Mei%22">Wang, Mei</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Knubovets%2C+Tanya%22">Knubovets, Tanya</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Scrivener%2C+Elaine%22">Scrivener, Elaine</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Golenko%2C+Eva%22">Golenko, Eva</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Krull%2C+Ira+S%2E%22">Krull, Ira S.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Schulz%2C+Michael%22">Schulz, Michael</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Heinz-Emil-Hauck%22">Heinz-Emil-Hauck</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Patton%2C+Wayne+F%2E%22">Patton, Wayne F.</searchLink><relatesTo>1</relatesTo><i> wayne.patton@perkinelmer.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Jun2007, Vol. 1155 Issue 1, p112-123. 12p.
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  Data: <searchLink fieldCode="DE" term="%22Chromatographic+analysis%22">Chromatographic analysis</searchLink><br /><searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Proteins%22">Proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink>
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  Data: Abstract: A novel strategy is presented for the fractionation of complex peptide mixtures using two-dimensional planar electrochromatography/thin-layer chromatography (2D PEC/TLC). Phosphopeptides migrate more slowly in the first dimension, based upon their anionic phosphate residues, and certain predominantly acidic phosphopeptides even migrate in the opposite direction, relative to the bulk of the peptides. Phosphopeptides are further distinguished based upon hydrophilicity in the second dimension. This permits a restricted region of the plate to be directly interrogated for the presence of phosphopeptides by mass spectrometry (MS). Phosphopeptide analysis from the plates by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)-MS and tandem MS enabled peptide sequencing and identification. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.chroma.2007.04.029
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      – Code: eng
        Text: English
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        PageCount: 12
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      – SubjectFull: Chromatographic analysis
        Type: general
      – SubjectFull: Peptides
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      – SubjectFull: Proteins
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      – TitleFull: Phosphopeptide analysis by directly coupling two-dimensional planar electrochromatography/thin-layer chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
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              M: 06
              Text: Jun2007
              Type: published
              Y: 2007
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