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Investigation of the ion-exchange properties of methacrylate-based mixed-mode monolithic stationary phases employed as stationary phases in capillary electrochromatography

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Title: Investigation of the ion-exchange properties of methacrylate-based mixed-mode monolithic stationary phases employed as stationary phases in capillary electrochromatography
Authors: Al-Rimawi, Fuad1, Pyell, Ute pyell@chemie.uni-marburg.de
Source: Journal of Chromatography A. Aug2007, Vol. 1160 Issue 1/2, p326-335. 10p.
Subjects: Methyl methacrylate, Sulfonic acids, Electrophoresis, Ion exchange (Chemistry)
Abstract: Abstract: The potential of methacrylate-based mixed-mode monolithic stationary phases bearing sulfonic acid groups for the separation of positively charged analytes (alkylanilines, amino acids, and peptides) by capillary electrochromatography (CEC) is investigated. The retention mechanism of protonated alkylanilines as positively charged model solutes on these negatively charged mixed-mode stationary phases is investigated by studying the influence of mobile phase and stationary phase parameters on the corrected retention factor which was calculated by taking the electrophoretic mobility of the solutes into consideration. It is shown that both solvophobic and ion-exchange interactions contribute to the retention of these analytes. The dependence of the corrected retention factor on (1) the concentration of the counter ion ammonium and (2) the number of methylene groups in the alkyl chain of the model analytes investigated shows clearly that a one-site model (solvophobic and ion-exchange interactions take place simultaneously at a single type of site) has to be taken to describe the retention behaviour observed. Comparison of the CEC separation of these charged analytes with electrophoretic mobilities determined by open-tubular capillary electrophoresis shows that mainly chromatographic interactions (solvophobic and ion-exchange interactions) are responsible for the selectivity observed in CEC, while the electrophoretic migration of these analytes plays only a minor role. [Copyright &y& Elsevier]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 25827039
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Investigation of the ion-exchange properties of methacrylate-based mixed-mode monolithic stationary phases employed as stationary phases in capillary electrochromatography
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  Data: <searchLink fieldCode="AR" term="%22Al-Rimawi%2C+Fuad%22">Al-Rimawi, Fuad</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pyell%2C+Ute%22">Pyell, Ute</searchLink><i> pyell@chemie.uni-marburg.de</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Aug2007, Vol. 1160 Issue 1/2, p326-335. 10p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Methyl+methacrylate%22">Methyl methacrylate</searchLink><br /><searchLink fieldCode="DE" term="%22Sulfonic+acids%22">Sulfonic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Electrophoresis%22">Electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Ion+exchange+%28Chemistry%29%22">Ion exchange (Chemistry)</searchLink>
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  Label: Abstract
  Group: Ab
  Data: Abstract: The potential of methacrylate-based mixed-mode monolithic stationary phases bearing sulfonic acid groups for the separation of positively charged analytes (alkylanilines, amino acids, and peptides) by capillary electrochromatography (CEC) is investigated. The retention mechanism of protonated alkylanilines as positively charged model solutes on these negatively charged mixed-mode stationary phases is investigated by studying the influence of mobile phase and stationary phase parameters on the corrected retention factor which was calculated by taking the electrophoretic mobility of the solutes into consideration. It is shown that both solvophobic and ion-exchange interactions contribute to the retention of these analytes. The dependence of the corrected retention factor on (1) the concentration of the counter ion ammonium and (2) the number of methylene groups in the alkyl chain of the model analytes investigated shows clearly that a one-site model (solvophobic and ion-exchange interactions take place simultaneously at a single type of site) has to be taken to describe the retention behaviour observed. Comparison of the CEC separation of these charged analytes with electrophoretic mobilities determined by open-tubular capillary electrophoresis shows that mainly chromatographic interactions (solvophobic and ion-exchange interactions) are responsible for the selectivity observed in CEC, while the electrophoretic migration of these analytes plays only a minor role. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.chroma.2007.05.035
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      – Code: eng
        Text: English
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        PageCount: 10
        StartPage: 326
    Subjects:
      – SubjectFull: Methyl methacrylate
        Type: general
      – SubjectFull: Sulfonic acids
        Type: general
      – SubjectFull: Electrophoresis
        Type: general
      – SubjectFull: Ion exchange (Chemistry)
        Type: general
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      – TitleFull: Investigation of the ion-exchange properties of methacrylate-based mixed-mode monolithic stationary phases employed as stationary phases in capillary electrochromatography
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              Text: Aug2007
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              Y: 2007
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            – TitleFull: Journal of Chromatography A
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