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Molecular typing of the Legionella pneumophila population isolated from several locations in a contaminated water network.

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Title: Molecular typing of the Legionella pneumophila population isolated from several locations in a contaminated water network.
Authors: Lepeuple, A.-S.1 anne-sophie.lepeuple@generale-des-eaux.net, Jovic, M.1, De Roubin, M.-R.1
Source: Water Science & Technology. 2004, Vol. 50 Issue 1, p281-285. 5p.
Subjects: Rapd technique, Legionella, Hot water, Diagnostic use of polymerase chain reaction, Water pollution, Water management
Abstract: The RAPD (random amplified polymorphic DNA) technique has been developed for the molecular typing of Legionella in order to characterise the populations of hot water systems. During this study, 22 primers were tested and the four most informative ones were selected. The optimisation of the PCR conditions allowed the setting up of a powerful discriminative genotyping method. Moreover, the definition of a quality management method allowed definition of the key steps and the number of replicates to ensure reproducibility of the RAPD pattern. The RAPD was used to study the hot water network of a building. Legionella colonies (91) were isolated from seven locations and genotyped. The diversity of the population in one sample could vary from one to seven different strains. The study of the traceability showed that, in most of the cases, different populations could be present at different locations of the same network. [ABSTRACT FROM AUTHOR]
Copyright of Water Science & Technology is the property of IWA Publishing and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 26699518
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Molecular typing of the Legionella pneumophila population isolated from several locations in a contaminated water network.
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  Data: <searchLink fieldCode="AR" term="%22Lepeuple%2C+A%2E-S%2E%22">Lepeuple, A.-S.</searchLink><relatesTo>1</relatesTo><i> anne-sophie.lepeuple@generale-des-eaux.net</i><br /><searchLink fieldCode="AR" term="%22Jovic%2C+M%2E%22">Jovic, M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22De+Roubin%2C+M%2E-R%2E%22">De Roubin, M.-R.</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Water+Science+%26+Technology%22">Water Science & Technology</searchLink>. 2004, Vol. 50 Issue 1, p281-285. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Rapd+technique%22">Rapd technique</searchLink><br /><searchLink fieldCode="DE" term="%22Legionella%22">Legionella</searchLink><br /><searchLink fieldCode="DE" term="%22Hot+water%22">Hot water</searchLink><br /><searchLink fieldCode="DE" term="%22Diagnostic+use+of+polymerase+chain+reaction%22">Diagnostic use of polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Water+pollution%22">Water pollution</searchLink><br /><searchLink fieldCode="DE" term="%22Water+management%22">Water management</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The RAPD (random amplified polymorphic DNA) technique has been developed for the molecular typing of Legionella in order to characterise the populations of hot water systems. During this study, 22 primers were tested and the four most informative ones were selected. The optimisation of the PCR conditions allowed the setting up of a powerful discriminative genotyping method. Moreover, the definition of a quality management method allowed definition of the key steps and the number of replicates to ensure reproducibility of the RAPD pattern. The RAPD was used to study the hot water network of a building. Legionella colonies (91) were isolated from seven locations and genotyped. The diversity of the population in one sample could vary from one to seven different strains. The study of the traceability showed that, in most of the cases, different populations could be present at different locations of the same network. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of Water Science & Technology is the property of IWA Publishing and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.2166/wst.2004.0068
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      – Code: eng
        Text: English
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        PageCount: 5
        StartPage: 281
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      – SubjectFull: Rapd technique
        Type: general
      – SubjectFull: Legionella
        Type: general
      – SubjectFull: Hot water
        Type: general
      – SubjectFull: Diagnostic use of polymerase chain reaction
        Type: general
      – SubjectFull: Water pollution
        Type: general
      – SubjectFull: Water management
        Type: general
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      – TitleFull: Molecular typing of the Legionella pneumophila population isolated from several locations in a contaminated water network.
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              Text: 2004
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