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Proteomic identification of tmRNA substrates.

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Title: Proteomic identification of tmRNA substrates.
Authors: Sue-Jean Hong1, Lessner, Faith H.1, Mahen, Elisabeth M.1, Keiler, Kenneth C.1 kkeiler@psu.edu
Source: Proceedings of the National Academy of Sciences of the United States of America. 10/23/2007, Vol. 104 Issue 43, p17128-17133. 6p.
Subjects: RNA, Ribosomes, Messenger RNA, DNA replication, DNA repair, Cell cycle
Abstract: The tmRNA-SmpB system releases ribosomes stalled on truncated mRNAs and tags the nascent polypeptides to target them for proteolysis. In many species, mutations that disrupt tmRNA activity cause defects in growth or development. In Cau!obacter crescentus cells lacking tmRNA activity there is a delay in the initiation of DNA replication, which disrupts the cell cycle. To understand the molecular basis for this phenotype, 73 C. crescentus proteins were identified that are tagged by tmRNA under normal growth conditions. Among these substrates, proteins involved in DNA replication, recombination, and repair were overrepresented, suggesting that misregulation of these factors in the absence of tmRNA activity might be responsible for the delay in initiation of DNA replication. Analysis of the tagging sites within these substrates revealed a conserved nucleotide motif 5′ of the tagging site, which is required for wild-type tmRNA tagging. [ABSTRACT FROM AUTHOR]
Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Proteomic identification of tmRNA substrates.
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  Data: <searchLink fieldCode="JN" term="%22Proceedings+of+the+National+Academy+of+Sciences+of+the+United+States+of+America%22">Proceedings of the National Academy of Sciences of the United States of America</searchLink>. 10/23/2007, Vol. 104 Issue 43, p17128-17133. 6p.
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  Data: <searchLink fieldCode="DE" term="%22RNA%22">RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Ribosomes%22">Ribosomes</searchLink><br /><searchLink fieldCode="DE" term="%22Messenger+RNA%22">Messenger RNA</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+replication%22">DNA replication</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+repair%22">DNA repair</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+cycle%22">Cell cycle</searchLink>
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  Data: The tmRNA-SmpB system releases ribosomes stalled on truncated mRNAs and tags the nascent polypeptides to target them for proteolysis. In many species, mutations that disrupt tmRNA activity cause defects in growth or development. In Cau!obacter crescentus cells lacking tmRNA activity there is a delay in the initiation of DNA replication, which disrupts the cell cycle. To understand the molecular basis for this phenotype, 73 C. crescentus proteins were identified that are tagged by tmRNA under normal growth conditions. Among these substrates, proteins involved in DNA replication, recombination, and repair were overrepresented, suggesting that misregulation of these factors in the absence of tmRNA activity might be responsible for the delay in initiation of DNA replication. Analysis of the tagging sites within these substrates revealed a conserved nucleotide motif 5′ of the tagging site, which is required for wild-type tmRNA tagging. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of Proceedings of the National Academy of Sciences of the United States of America is the property of National Academy of Sciences and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1073/pnas.0707671104
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 6
        StartPage: 17128
    Subjects:
      – SubjectFull: RNA
        Type: general
      – SubjectFull: Ribosomes
        Type: general
      – SubjectFull: Messenger RNA
        Type: general
      – SubjectFull: DNA replication
        Type: general
      – SubjectFull: DNA repair
        Type: general
      – SubjectFull: Cell cycle
        Type: general
    Titles:
      – TitleFull: Proteomic identification of tmRNA substrates.
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            NameFull: Sue-Jean Hong
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            NameFull: Lessner, Faith H.
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            NameFull: Mahen, Elisabeth M.
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            NameFull: Keiler, Kenneth C.
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            – D: 23
              M: 10
              Text: 10/23/2007
              Type: published
              Y: 2007
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              Value: 43
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            – TitleFull: Proceedings of the National Academy of Sciences of the United States of America
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