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Adsorption and activities of lipases on synthetic beidellite clays with variable composition

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Title: Adsorption and activities of lipases on synthetic beidellite clays with variable composition
Authors: Secundo, F.1, Miehé-Brendlé, J.2, Chelaru, Carmen3, Ferrandi, Erica Elisa1, Dumitriu, Emil4 edumitri@ch.tuiasi.ro
Source: Microporous & Mesoporous Materials. Mar2008, Vol. 109 Issue 1-3, p350-361. 12p.
Subjects: Lipases, Beidellite, Hydrolases, Solution (Chemistry)
Abstract: Abstract: Synthetic beidellite clays with variable Si/Al ratio in the tetrahedral layers were prepared by hydrothermal crystallization in an acid fluoride medium. The clay materials were characterized by powder-XRD, MAS NMR, BET specific area surfaces and chemical analysis. These samples were used as support for the immobilization of lipase B from Candida antarctica and lipase from Burkholderia cepacia by adsorption. The amount of lipase immobilized on the clay surface increased with decreasing level of aluminium substitution in the tetrahedral layer. The immobilization percentage was significantly higher for CALB (even for three times in the case of high siliceous clays) than those of BCL, when a sample of clay with the same composition is used as support. The catalytic properties of supported enzymes were investigated using the transesterification of vinyl acetate with 1-octanol as test reaction. The specific activity of the immobilized lipases depends on both the clay composition – the samples with low content of aluminum exhibited the highest specific activity-, and the amount of adsorbed enzyme – it increases as the amount of enzyme decreases. Also, the catalytic activity of lipase deposited on H+-beidellite is higher than those of enzyme deposited on the Na+ form. [Copyright &y& Elsevier]
Copyright of Microporous & Mesoporous Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 28079516
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PubTypeId: academicJournal
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  Data: Adsorption and activities of lipases on synthetic beidellite clays with variable composition
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  Data: <searchLink fieldCode="AR" term="%22Secundo%2C+F%2E%22">Secundo, F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Miehé-Brendlé%2C+J%2E%22">Miehé-Brendlé, J.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Chelaru%2C+Carmen%22">Chelaru, Carmen</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Ferrandi%2C+Erica+Elisa%22">Ferrandi, Erica Elisa</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Dumitriu%2C+Emil%22">Dumitriu, Emil</searchLink><relatesTo>4</relatesTo><i> edumitri@ch.tuiasi.ro</i>
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  Data: <searchLink fieldCode="JN" term="%22Microporous+%26+Mesoporous+Materials%22">Microporous & Mesoporous Materials</searchLink>. Mar2008, Vol. 109 Issue 1-3, p350-361. 12p.
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  Data: <searchLink fieldCode="DE" term="%22Lipases%22">Lipases</searchLink><br /><searchLink fieldCode="DE" term="%22Beidellite%22">Beidellite</searchLink><br /><searchLink fieldCode="DE" term="%22Hydrolases%22">Hydrolases</searchLink><br /><searchLink fieldCode="DE" term="%22Solution+%28Chemistry%29%22">Solution (Chemistry)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Synthetic beidellite clays with variable Si/Al ratio in the tetrahedral layers were prepared by hydrothermal crystallization in an acid fluoride medium. The clay materials were characterized by powder-XRD, MAS NMR, BET specific area surfaces and chemical analysis. These samples were used as support for the immobilization of lipase B from Candida antarctica and lipase from Burkholderia cepacia by adsorption. The amount of lipase immobilized on the clay surface increased with decreasing level of aluminium substitution in the tetrahedral layer. The immobilization percentage was significantly higher for CALB (even for three times in the case of high siliceous clays) than those of BCL, when a sample of clay with the same composition is used as support. The catalytic properties of supported enzymes were investigated using the transesterification of vinyl acetate with 1-octanol as test reaction. The specific activity of the immobilized lipases depends on both the clay composition – the samples with low content of aluminum exhibited the highest specific activity-, and the amount of adsorbed enzyme – it increases as the amount of enzyme decreases. Also, the catalytic activity of lipase deposited on H+-beidellite is higher than those of enzyme deposited on the Na+ form. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Microporous & Mesoporous Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.micromeso.2007.05.032
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 12
        StartPage: 350
    Subjects:
      – SubjectFull: Lipases
        Type: general
      – SubjectFull: Beidellite
        Type: general
      – SubjectFull: Hydrolases
        Type: general
      – SubjectFull: Solution (Chemistry)
        Type: general
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      – TitleFull: Adsorption and activities of lipases on synthetic beidellite clays with variable composition
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            NameFull: Secundo, F.
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            NameFull: Miehé-Brendlé, J.
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            NameFull: Chelaru, Carmen
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            NameFull: Ferrandi, Erica Elisa
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            NameFull: Dumitriu, Emil
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              M: 03
              Text: Mar2008
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              Y: 2008
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              Value: 109
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