View in EDS

Cytotoxicity of metal ions to human oligodendroglial cells and human gingival fibroblasts assessed by mitochondrial dehydrogenase activity

Saved in:
Bibliographic Details
Title: Cytotoxicity of metal ions to human oligodendroglial cells and human gingival fibroblasts assessed by mitochondrial dehydrogenase activity
Authors: Issa, Y.1, Brunton, P.2 p.a.brunton@leeds.ac.uk, Waters, C.M.3, Watts, D.C.1
Source: Dental Materials. Feb2008, Vol. 24 Issue 2, p281-287. 7p.
Subjects: Metal ions, Oligodendroglia, Cadmium, Metals
Abstract: Abstract: Objectives: To assess the variable concentrations of several metal salts on human oligodendrocyte MO3.13 and human gingival fibroblasts HGF and to enable any difference in cell type sensitivity to be examined. Methods: Cytotoxicity was measured as mitochondrial dehydrogenase activity assessed by MTT assay. The mean of the 50% response (TC50) was calculated by using equation-fitting software (TableCurve 2D). Results: The results of the MTT assay showed that metal ions induce reproducible cytotoxic effects in MO3.13 oligodendroglia and human gingival fibroblasts, that is dose dependent on the tested agent. Cadmium relatively showed the highest cytotoxic effects on MO3.13 cells (TC50 9.8μM) whereas mercury showed the highest cytotoxic effects on HGF (TC50 74μM) comparing with other tested metals. The two cell types responded differently. MO3.13 cells were more sensitive than the HGF to most of the metals. Conclusion: Metals have a wide range of toxicity to human oligodendroglial cells (MO3.13) and human gingival fibroblasts. Fortunately, however, in vivo the normal levels of these metals are much lower than those determined as toxic in vitro. [Copyright &y& Elsevier]
Copyright of Dental Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 28116855
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Cytotoxicity of metal ions to human oligodendroglial cells and human gingival fibroblasts assessed by mitochondrial dehydrogenase activity
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Issa%2C+Y%2E%22">Issa, Y.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Brunton%2C+P%2E%22">Brunton, P.</searchLink><relatesTo>2</relatesTo><i> p.a.brunton@leeds.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Waters%2C+C%2EM%2E%22">Waters, C.M.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Watts%2C+D%2EC%2E%22">Watts, D.C.</searchLink><relatesTo>1</relatesTo>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Dental+Materials%22">Dental Materials</searchLink>. Feb2008, Vol. 24 Issue 2, p281-287. 7p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Metal+ions%22">Metal ions</searchLink><br /><searchLink fieldCode="DE" term="%22Oligodendroglia%22">Oligodendroglia</searchLink><br /><searchLink fieldCode="DE" term="%22Cadmium%22">Cadmium</searchLink><br /><searchLink fieldCode="DE" term="%22Metals%22">Metals</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Objectives: To assess the variable concentrations of several metal salts on human oligodendrocyte MO3.13 and human gingival fibroblasts HGF and to enable any difference in cell type sensitivity to be examined. Methods: Cytotoxicity was measured as mitochondrial dehydrogenase activity assessed by MTT assay. The mean of the 50% response (TC50) was calculated by using equation-fitting software (TableCurve 2D). Results: The results of the MTT assay showed that metal ions induce reproducible cytotoxic effects in MO3.13 oligodendroglia and human gingival fibroblasts, that is dose dependent on the tested agent. Cadmium relatively showed the highest cytotoxic effects on MO3.13 cells (TC50 9.8μM) whereas mercury showed the highest cytotoxic effects on HGF (TC50 74μM) comparing with other tested metals. The two cell types responded differently. MO3.13 cells were more sensitive than the HGF to most of the metals. Conclusion: Metals have a wide range of toxicity to human oligodendroglial cells (MO3.13) and human gingival fibroblasts. Fortunately, however, in vivo the normal levels of these metals are much lower than those determined as toxic in vitro. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Dental Materials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=28116855
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.dental.2007.09.010
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 7
        StartPage: 281
    Subjects:
      – SubjectFull: Metal ions
        Type: general
      – SubjectFull: Oligodendroglia
        Type: general
      – SubjectFull: Cadmium
        Type: general
      – SubjectFull: Metals
        Type: general
    Titles:
      – TitleFull: Cytotoxicity of metal ions to human oligodendroglial cells and human gingival fibroblasts assessed by mitochondrial dehydrogenase activity
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Issa, Y.
      – PersonEntity:
          Name:
            NameFull: Brunton, P.
      – PersonEntity:
          Name:
            NameFull: Waters, C.M.
      – PersonEntity:
          Name:
            NameFull: Watts, D.C.
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 02
              Text: Feb2008
              Type: published
              Y: 2008
          Identifiers:
            – Type: issn-print
              Value: 01095641
          Numbering:
            – Type: volume
              Value: 24
            – Type: issue
              Value: 2
          Titles:
            – TitleFull: Dental Materials
              Type: main
ResultId 1