Genetic organization of pha gene locus affects phaC expression, poly(hydroxyalkanoate) composition and granule morphology in Pseudomonas corrugata.
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| Title: | Genetic organization of pha gene locus affects phaC expression, poly(hydroxyalkanoate) composition and granule morphology in Pseudomonas corrugata. |
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| Authors: | Solaiman, Daniel K. Y.1 dan.solaiman@ars.usda.gov, Ashby, Richard D.1, Licciardello, Grazia2, Catara, Vittoria2 vcatara@unict.it |
| Source: | Journal of Industrial Microbiology & Biotechnology. Feb2008, Vol. 35 Issue 2, p111-120. 10p. 1 Color Photograph, 1 Black and White Photograph, 3 Diagrams, 2 Charts, 1 Graph. |
| Subjects: | Poly-beta-hydroxyalkanoates, Genes, Oleic acid, Monounsaturated fatty acids, Organic synthesis, Biochemical engineering, Biochemistry, Pseudomonadaceae, Nucleotide sequence, Nucleotide analysis |
| Abstract: | The complete sequence of the pha locus responsible for the biosynthesis of poly(hydroxyalkanoates) (PHAs) in Pseudomonas corrugata 388 was determined. As with the other known pseudomonad pha gene loci, the one in P. corrugata 388 also consists of phaC1 (1,680 bps; PHA synthase 1), phaZ (858 bp; PHA depolymerase) and phaC2 (1,683 bp; PHA synthase 2) genes. A BLAST search showed that the nucleotide sequences of these genes and the amino-acid sequences of their respective gene products are homologous to those of P. corrugata CFBP5454 and P. mediterranea CFBP5447. A putative intrinsic transcription terminator consisting of a dyad symmetry (24 bp; Δ G = −41.8 kcals) that precedes a stretch of dA residues was located in the phaC1– phaZ intergenic region. P. corrugata mutant-clones XI 32-1 and XI 32-4 were constructed in which this intergenic region was replaced with a selectable kanamycin-resistance gene. These mutant clones when grown on oleic acid for 48 h showed 4.7–to 7.0-fold increases of phaC1 and phaC2 relative expression in comparison to the initial inoculants, whereas the parental strain showed only 1.2- to 1.4-fold increases. Furthermore, in comparison to parental P. corrugata with only a few large PHA inclusion bodies, the mutants grown on oleic acid produce numerous smaller PHA granules that line the periphery of the cells. With glucose as a substrate, XI 32-1 and XI 32-4 clones produce mcl-PHA with a high content (26–31 mol%) of the mono-unsaturated 3-hydroxydodecenoate as a repeat-unit monomer. Our results show for the first time the effects of the phaC1– phaZ intergenic region on the substrate-dependent temporal expression of phaC1 and phaC2 genes, the repeat-unit composition of mcl-PHA, and the morphology of the PHA granules. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Industrial Microbiology & Biotechnology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 28126062 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Genetic organization of pha gene locus affects phaC expression, poly(hydroxyalkanoate) composition and granule morphology in Pseudomonas corrugata. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Solaiman%2C+Daniel+K%2E+Y%2E%22">Solaiman, Daniel K. Y.</searchLink><relatesTo>1</relatesTo><i> dan.solaiman@ars.usda.gov</i><br /><searchLink fieldCode="AR" term="%22Ashby%2C+Richard+D%2E%22">Ashby, Richard D.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Licciardello%2C+Grazia%22">Licciardello, Grazia</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Catara%2C+Vittoria%22">Catara, Vittoria</searchLink><relatesTo>2</relatesTo><i> vcatara@unict.it</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Industrial+Microbiology+%26+Biotechnology%22">Journal of Industrial Microbiology & Biotechnology</searchLink>. Feb2008, Vol. 35 Issue 2, p111-120. 10p. 1 Color Photograph, 1 Black and White Photograph, 3 Diagrams, 2 Charts, 1 Graph. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Poly-beta-hydroxyalkanoates%22">Poly-beta-hydroxyalkanoates</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Oleic+acid%22">Oleic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Monounsaturated+fatty+acids%22">Monounsaturated fatty acids</searchLink><br /><searchLink fieldCode="DE" term="%22Organic+synthesis%22">Organic synthesis</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemical+engineering%22">Biochemical engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudomonadaceae%22">Pseudomonadaceae</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotide+sequence%22">Nucleotide sequence</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotide+analysis%22">Nucleotide analysis</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The complete sequence of the pha locus responsible for the biosynthesis of poly(hydroxyalkanoates) (PHAs) in Pseudomonas corrugata 388 was determined. As with the other known pseudomonad pha gene loci, the one in P. corrugata 388 also consists of phaC1 (1,680 bps; PHA synthase 1), phaZ (858 bp; PHA depolymerase) and phaC2 (1,683 bp; PHA synthase 2) genes. A BLAST search showed that the nucleotide sequences of these genes and the amino-acid sequences of their respective gene products are homologous to those of P. corrugata CFBP5454 and P. mediterranea CFBP5447. A putative intrinsic transcription terminator consisting of a dyad symmetry (24 bp; Δ G = −41.8 kcals) that precedes a stretch of dA residues was located in the phaC1– phaZ intergenic region. P. corrugata mutant-clones XI 32-1 and XI 32-4 were constructed in which this intergenic region was replaced with a selectable kanamycin-resistance gene. These mutant clones when grown on oleic acid for 48 h showed 4.7–to 7.0-fold increases of phaC1 and phaC2 relative expression in comparison to the initial inoculants, whereas the parental strain showed only 1.2- to 1.4-fold increases. Furthermore, in comparison to parental P. corrugata with only a few large PHA inclusion bodies, the mutants grown on oleic acid produce numerous smaller PHA granules that line the periphery of the cells. With glucose as a substrate, XI 32-1 and XI 32-4 clones produce mcl-PHA with a high content (26–31 mol%) of the mono-unsaturated 3-hydroxydodecenoate as a repeat-unit monomer. Our results show for the first time the effects of the phaC1– phaZ intergenic region on the substrate-dependent temporal expression of phaC1 and phaC2 genes, the repeat-unit composition of mcl-PHA, and the morphology of the PHA granules. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Industrial Microbiology & Biotechnology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10295-007-0272-y Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 111 Subjects: – SubjectFull: Poly-beta-hydroxyalkanoates Type: general – SubjectFull: Genes Type: general – SubjectFull: Oleic acid Type: general – SubjectFull: Monounsaturated fatty acids Type: general – SubjectFull: Organic synthesis Type: general – SubjectFull: Biochemical engineering Type: general – SubjectFull: Biochemistry Type: general – SubjectFull: Pseudomonadaceae Type: general – SubjectFull: Nucleotide sequence Type: general – SubjectFull: Nucleotide analysis Type: general Titles: – TitleFull: Genetic organization of pha gene locus affects phaC expression, poly(hydroxyalkanoate) composition and granule morphology in Pseudomonas corrugata. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Solaiman, Daniel K. Y. – PersonEntity: Name: NameFull: Ashby, Richard D. – PersonEntity: Name: NameFull: Licciardello, Grazia – PersonEntity: Name: NameFull: Catara, Vittoria IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 02 Text: Feb2008 Type: published Y: 2008 Identifiers: – Type: issn-print Value: 13675435 Numbering: – Type: volume Value: 35 – Type: issue Value: 2 Titles: – TitleFull: Journal of Industrial Microbiology & Biotechnology Type: main |
| ResultId | 1 |
