Preparation of biomolecule gel matrices for electron microscopy

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Title: Preparation of biomolecule gel matrices for electron microscopy
Authors: Padalkar, Sonal1, Zhao, Jianxiu2, Stuart, Kate3, Panitch, Alyssa3, Rickus, Jenna2,3, Stanciu, Lia1,3 lstanciu@purdue.edu
Source: Ultramicroscopy. Mar2008, Vol. 108 Issue 4, p309-313. 5p.
Subjects: Colloids, Electron microscopy, Particles (Nuclear physics), Universal algebra
Abstract: Abstract: We report a new sample preparation method that allows the direct transmission electron microscopy evaluation of the architectural characteristics of biomolecules entrapped in gel matrices. We demonstrate that this sample preparation technique can be used for the identification and ultrastructural characterization of liposomes, collagen I and collagen III embedded in gel matrices, and has the potential to be useful for transmission electron microscopy (TEM) characterization of other biomolecule–gel matrix systems. [Copyright &y& Elsevier]
Copyright of Ultramicroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 29961377
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Preparation of biomolecule gel matrices for electron microscopy
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  Data: <searchLink fieldCode="AR" term="%22Padalkar%2C+Sonal%22">Padalkar, Sonal</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhao%2C+Jianxiu%22">Zhao, Jianxiu</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Stuart%2C+Kate%22">Stuart, Kate</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Panitch%2C+Alyssa%22">Panitch, Alyssa</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Rickus%2C+Jenna%22">Rickus, Jenna</searchLink><relatesTo>2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Stanciu%2C+Lia%22">Stanciu, Lia</searchLink><relatesTo>1,3</relatesTo><i> lstanciu@purdue.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Ultramicroscopy%22">Ultramicroscopy</searchLink>. Mar2008, Vol. 108 Issue 4, p309-313. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Colloids%22">Colloids</searchLink><br /><searchLink fieldCode="DE" term="%22Electron+microscopy%22">Electron microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Particles+%28Nuclear+physics%29%22">Particles (Nuclear physics)</searchLink><br /><searchLink fieldCode="DE" term="%22Universal+algebra%22">Universal algebra</searchLink>
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  Label: Abstract
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  Data: Abstract: We report a new sample preparation method that allows the direct transmission electron microscopy evaluation of the architectural characteristics of biomolecules entrapped in gel matrices. We demonstrate that this sample preparation technique can be used for the identification and ultrastructural characterization of liposomes, collagen I and collagen III embedded in gel matrices, and has the potential to be useful for transmission electron microscopy (TEM) characterization of other biomolecule–gel matrix systems. [Copyright &y& Elsevier]
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  Data: <i>Copyright of Ultramicroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.ultramic.2007.04.004
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      – Code: eng
        Text: English
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        PageCount: 5
        StartPage: 309
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      – SubjectFull: Colloids
        Type: general
      – SubjectFull: Electron microscopy
        Type: general
      – SubjectFull: Particles (Nuclear physics)
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      – SubjectFull: Universal algebra
        Type: general
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      – TitleFull: Preparation of biomolecule gel matrices for electron microscopy
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            NameFull: Zhao, Jianxiu
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            NameFull: Rickus, Jenna
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              M: 03
              Text: Mar2008
              Type: published
              Y: 2008
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