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Influence of volume reduction and cryopreservation methodologies on quality of thawed umbilical cord blood units for transplantation

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Title: Influence of volume reduction and cryopreservation methodologies on quality of thawed umbilical cord blood units for transplantation
Authors: Solves, Pilar solves_pil@gva.es, Mirabet, Vicente1, Planelles, Dolores1, Carbonell-Uberos, Francisco1, Roig, Roberto1
Source: Cryobiology. Apr2008, Vol. 56 Issue 2, p152-158. 7p.
Subjects: Cord blood, Blood transfusion, Blood, Body fluids
Abstract: Abstract: Introduction: Although there is considerable variability in methodology among umbilical cord blood banks, their common goal is to achieve optimal product quality for transplantation. Cryopreservation is a critical issue for a long-term maintenance of cord blood viability and colony-forming capacities. Materials and methods: We designed a prospective study to compare controlled (CRF) vs. non-controlled freezing (URF) of volume-reduced cord blood units. In addition, the influence of hydroxy ethyl starch (HES) on cryopreservation was also assayed. To assess the efficiency of protocols used, cell recoveries were measured and the presence of hematopoietic colony-forming units was quantified. Results: In the study phase, we observed similar CB haematopoietc recoveries for CRF and URF strategies, except for TNC recovery that was better for HES volume reduced CB units in the URF group. When we analysed the data of routine processed CB units in samples from satellite cryovials, we found better BFU-E, CFU-GM, CFU-GEMM and CFU recoveries for those units processed with HES than without HES, in an URF manner. Conclusions: URF of CB units is a cryopreservation procedure that allows similar hematopoietic progenitor recoveries than CRF with programmed devices. However, our study suggests that those banks that cryopreserve CB units in a URF manner should use HES for volume reduction. On the other hand, for CRF cryopreservation methodology volume reduction with and without HES are equally useful. [Copyright &y& Elsevier]
Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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  Data: Influence of volume reduction and cryopreservation methodologies on quality of thawed umbilical cord blood units for transplantation
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  Data: <searchLink fieldCode="AR" term="%22Solves%2C+Pilar%22">Solves, Pilar</searchLink><i> solves_pil@gva.es</i><br /><searchLink fieldCode="AR" term="%22Mirabet%2C+Vicente%22">Mirabet, Vicente</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Planelles%2C+Dolores%22">Planelles, Dolores</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Carbonell-Uberos%2C+Francisco%22">Carbonell-Uberos, Francisco</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Roig%2C+Roberto%22">Roig, Roberto</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Cryobiology%22">Cryobiology</searchLink>. Apr2008, Vol. 56 Issue 2, p152-158. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Cord+blood%22">Cord blood</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+transfusion%22">Blood transfusion</searchLink><br /><searchLink fieldCode="DE" term="%22Blood%22">Blood</searchLink><br /><searchLink fieldCode="DE" term="%22Body+fluids%22">Body fluids</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Introduction: Although there is considerable variability in methodology among umbilical cord blood banks, their common goal is to achieve optimal product quality for transplantation. Cryopreservation is a critical issue for a long-term maintenance of cord blood viability and colony-forming capacities. Materials and methods: We designed a prospective study to compare controlled (CRF) vs. non-controlled freezing (URF) of volume-reduced cord blood units. In addition, the influence of hydroxy ethyl starch (HES) on cryopreservation was also assayed. To assess the efficiency of protocols used, cell recoveries were measured and the presence of hematopoietic colony-forming units was quantified. Results: In the study phase, we observed similar CB haematopoietc recoveries for CRF and URF strategies, except for TNC recovery that was better for HES volume reduced CB units in the URF group. When we analysed the data of routine processed CB units in samples from satellite cryovials, we found better BFU-E, CFU-GM, CFU-GEMM and CFU recoveries for those units processed with HES than without HES, in an URF manner. Conclusions: URF of CB units is a cryopreservation procedure that allows similar hematopoietic progenitor recoveries than CRF with programmed devices. However, our study suggests that those banks that cryopreserve CB units in a URF manner should use HES for volume reduction. On the other hand, for CRF cryopreservation methodology volume reduction with and without HES are equally useful. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Cryobiology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.cryobiol.2008.02.002
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              Text: Apr2008
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