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New perspective of diabetes response to an antioxidant treatment through metabolic fingerprinting of urine by capillary electrophoresis

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Title: New perspective of diabetes response to an antioxidant treatment through metabolic fingerprinting of urine by capillary electrophoresis
Authors: Vallejo, M.1, Angulo, S.1, García-Martínez, D.1, García, A.1, Barbas, C. cbarbas@ceu.es
Source: Journal of Chromatography A. Apr2008, Vol. 1187 Issue 1/2, p267-274. 8p.
Subjects: Diabetes, Antioxidants, Capillary electrophoresis, Metabolites
Abstract: Abstract: Benefits of capillary electrophoresis to provide a comprehensive snapshot of multiple metabolites in biological samples have been exploited. Afterwards, multivariate statistical methods can be employed in order to mine additional information from the data. Urine fingerprints of control and diabetic rats have shown the clear effects of an antioxidant treatment on diabetic animals, which were not seen in controls, in a rapid, simple and cost-effective way without identifying a single marker. The procedure involves the measurement of samples with a relatively inexpensive tool such as CE-UV, without any previous treatment other than filtration and the application of chemometric tools [PCA (principal components analysis) and PLS-DA (partial least squares discriminant analysis)]. Data pre-treatment of electrophoretic profiles (alignment, normalization and baseline correction) has shown to be key for further chemometric treatment. Once developed, the methodology can easily be applied for a rapid in vivo screening of extracts with potential in vitro activity. Classification was supported by that produced after PCA and PLS-DA of target variables obtained with selectively designed, time and reagent consuming methods. [Copyright &y& Elsevier]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 31491766
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: New perspective of diabetes response to an antioxidant treatment through metabolic fingerprinting of urine by capillary electrophoresis
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  Data: <searchLink fieldCode="AR" term="%22Vallejo%2C+M%2E%22">Vallejo, M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Angulo%2C+S%2E%22">Angulo, S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22García-Martínez%2C+D%2E%22">García-Martínez, D.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22García%2C+A%2E%22">García, A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Barbas%2C+C%2E%22">Barbas, C.</searchLink><i> cbarbas@ceu.es</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+A%22">Journal of Chromatography A</searchLink>. Apr2008, Vol. 1187 Issue 1/2, p267-274. 8p.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Diabetes%22">Diabetes</searchLink><br /><searchLink fieldCode="DE" term="%22Antioxidants%22">Antioxidants</searchLink><br /><searchLink fieldCode="DE" term="%22Capillary+electrophoresis%22">Capillary electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Metabolites%22">Metabolites</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: Benefits of capillary electrophoresis to provide a comprehensive snapshot of multiple metabolites in biological samples have been exploited. Afterwards, multivariate statistical methods can be employed in order to mine additional information from the data. Urine fingerprints of control and diabetic rats have shown the clear effects of an antioxidant treatment on diabetic animals, which were not seen in controls, in a rapid, simple and cost-effective way without identifying a single marker. The procedure involves the measurement of samples with a relatively inexpensive tool such as CE-UV, without any previous treatment other than filtration and the application of chemometric tools [PCA (principal components analysis) and PLS-DA (partial least squares discriminant analysis)]. Data pre-treatment of electrophoretic profiles (alignment, normalization and baseline correction) has shown to be key for further chemometric treatment. Once developed, the methodology can easily be applied for a rapid in vivo screening of extracts with potential in vitro activity. Classification was supported by that produced after PCA and PLS-DA of target variables obtained with selectively designed, time and reagent consuming methods. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=31491766
RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.chroma.2008.02.024
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      – Code: eng
        Text: English
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        PageCount: 8
        StartPage: 267
    Subjects:
      – SubjectFull: Diabetes
        Type: general
      – SubjectFull: Antioxidants
        Type: general
      – SubjectFull: Capillary electrophoresis
        Type: general
      – SubjectFull: Metabolites
        Type: general
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      – TitleFull: New perspective of diabetes response to an antioxidant treatment through metabolic fingerprinting of urine by capillary electrophoresis
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            NameFull: Angulo, S.
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            NameFull: García-Martínez, D.
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            NameFull: García, A.
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            NameFull: Barbas, C.
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              M: 04
              Text: Apr2008
              Type: published
              Y: 2008
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              Value: 1187
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            – TitleFull: Journal of Chromatography A
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