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Ndell Controls the Dynein-mediated Transport of Vimentin during Neurite Outgrowth.

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Title: Ndell Controls the Dynein-mediated Transport of Vimentin during Neurite Outgrowth.
Authors: Suyeon Shim1,2,3, Samuels, Benjamin Adam4, Jian Wang1,2,3, Neumayer, Gernot1,2,3, Belzil, Camille1,2,3, Ayala, Ramses4, Yang Shi5, Yujiang Shi5, Li-huei Tsai4, Nguyen, Minh Dang1,2,3 mdnguyen@ucalgary.ca
Source: Journal of Biological Chemistry. 5/2/2008, Vol. 283 Issue 18, p12232-12240. 9p. 5 Diagrams, 1 Graph.
Subjects: Dynein, Aspergillus nidulans, Cytoskeleton, Adenosine triphosphatase, Aspergillus
Abstract: Ndel1, the mammalian homologue of the Aspergillus nidulans NudE, is emergently viewed as an integrator of the cytoskeleton. By regulating the dynamics of microtubules and assembly of neuronal intermediate filaments (IFs), Ndel1 promotes neurite outgrowth, neuronal migration, and cell integrity (1-6). To further understand the roles of Ndel1 in cytoskeletal dynamics, we performed a tandem affinity purification of Ndel1-interacting proteins. We isolated a novel Ndel1 molecular complex composed of the IF vimentin, the molecular motor dynein, the lissencephaly protein Lis1, and the cis-Golgi-associated protein αCOP. Ndel1 promotes the interaction between Lis1, αCOP, and the vimentin-dynein complex. The functional result of this complex is activation of dynein-mediated transport of vimentin. A loss of Ndel1 functions by RNA interference fails to incorporate Lis1/αCOP in the complex, reduces the transport of vimentin, and culminates in IF accumulations and altered neuritogenesis. Our findings reveal a novel regulatory mechanism of vimentin transport during neurite extension that may have implications in diseases featuring transport/trafficking defects and impaired regeneration. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 32148423
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Ndell Controls the Dynein-mediated Transport of Vimentin during Neurite Outgrowth.
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  Data: <searchLink fieldCode="AR" term="%22Suyeon+Shim%22">Suyeon Shim</searchLink><relatesTo>1,2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Samuels%2C+Benjamin+Adam%22">Samuels, Benjamin Adam</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Jian+Wang%22">Jian Wang</searchLink><relatesTo>1,2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Neumayer%2C+Gernot%22">Neumayer, Gernot</searchLink><relatesTo>1,2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Belzil%2C+Camille%22">Belzil, Camille</searchLink><relatesTo>1,2,3</relatesTo><br /><searchLink fieldCode="AR" term="%22Ayala%2C+Ramses%22">Ayala, Ramses</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Yang+Shi%22">Yang Shi</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Yujiang+Shi%22">Yujiang Shi</searchLink><relatesTo>5</relatesTo><br /><searchLink fieldCode="AR" term="%22Li-huei+Tsai%22">Li-huei Tsai</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Nguyen%2C+Minh+Dang%22">Nguyen, Minh Dang</searchLink><relatesTo>1,2,3</relatesTo><i> mdnguyen@ucalgary.ca</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 5/2/2008, Vol. 283 Issue 18, p12232-12240. 9p. 5 Diagrams, 1 Graph.
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  Data: <searchLink fieldCode="DE" term="%22Dynein%22">Dynein</searchLink><br /><searchLink fieldCode="DE" term="%22Aspergillus+nidulans%22">Aspergillus nidulans</searchLink><br /><searchLink fieldCode="DE" term="%22Cytoskeleton%22">Cytoskeleton</searchLink><br /><searchLink fieldCode="DE" term="%22Adenosine+triphosphatase%22">Adenosine triphosphatase</searchLink><br /><searchLink fieldCode="DE" term="%22Aspergillus%22">Aspergillus</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Ndel1, the mammalian homologue of the Aspergillus nidulans NudE, is emergently viewed as an integrator of the cytoskeleton. By regulating the dynamics of microtubules and assembly of neuronal intermediate filaments (IFs), Ndel1 promotes neurite outgrowth, neuronal migration, and cell integrity (1-6). To further understand the roles of Ndel1 in cytoskeletal dynamics, we performed a tandem affinity purification of Ndel1-interacting proteins. We isolated a novel Ndel1 molecular complex composed of the IF vimentin, the molecular motor dynein, the lissencephaly protein Lis1, and the cis-Golgi-associated protein αCOP. Ndel1 promotes the interaction between Lis1, αCOP, and the vimentin-dynein complex. The functional result of this complex is activation of dynein-mediated transport of vimentin. A loss of Ndel1 functions by RNA interference fails to incorporate Lis1/αCOP in the complex, reduces the transport of vimentin, and culminates in IF accumulations and altered neuritogenesis. Our findings reveal a novel regulatory mechanism of vimentin transport during neurite extension that may have implications in diseases featuring transport/trafficking defects and impaired regeneration. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1074/jbc.M710200200
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        Text: English
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      Pagination:
        PageCount: 9
        StartPage: 12232
    Subjects:
      – SubjectFull: Dynein
        Type: general
      – SubjectFull: Aspergillus nidulans
        Type: general
      – SubjectFull: Cytoskeleton
        Type: general
      – SubjectFull: Adenosine triphosphatase
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      – SubjectFull: Aspergillus
        Type: general
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      – TitleFull: Ndell Controls the Dynein-mediated Transport of Vimentin during Neurite Outgrowth.
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              M: 05
              Text: 5/2/2008
              Type: published
              Y: 2008
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