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A FTIR microspectroscopy study of autolysis in cells of the wine yeast Saccharomyces cerevisiae

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Title: A FTIR microspectroscopy study of autolysis in cells of the wine yeast Saccharomyces cerevisiae
Authors: Burattini, E.1, Cavagna, M.2, Dell’Anna, R.3, Malvezzi Campeggi, F.1, Monti, F.1 francesca.monti@univr.it, Rossi, F.4, Torriani, S.2
Source: Vibrational Spectroscopy. Jul2008, Vol. 47 Issue 2, p139-147. 9p.
Subjects: Fourier transform infrared spectroscopy, Autolysis, Yeast, Saccharomyces cerevisiae
Abstract: Abstract: The present paper reports the first application of FTIR microspectroscopy in the mid-infrared range to study the major biochemical changes associated with autolysis in yeast cells. Measurements were done both in transmission and in attenuated total reflection (ATR) mode on cells of Saccharomyces cerevisiae strain EC1118 before and after induction of the autolytic process in a model wine medium and in a Chardonnay base wine. Unsupervised multivariate statistical analysis (Hierarchical Cluster Analysis and Principal Component Analysis), as well as accurate spectral analysis based on curve fitting, were applied to the acquired spectra. The spectral behaviour of S. cerevisiae in the model and base wines was found to be the same. A detailed interpretation of absorption bands was given by reference to the literature and through comparison of transmission and ATR spectra. It was shown that FTIR microspectroscopy is a rapid and accurate tool to simultaneously probe the major biochemical events associated with the autolytic process. Moreover, the intrinsically higher sensitivity of ATR with respect to transmission spectra in analyzing autolysis was also demonstrated. [Copyright &y& Elsevier]
Copyright of Vibrational Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
An: 32555554
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PubTypeId: academicJournal
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  Data: A FTIR microspectroscopy study of autolysis in cells of the wine yeast Saccharomyces cerevisiae
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  Data: <searchLink fieldCode="AR" term="%22Burattini%2C+E%2E%22">Burattini, E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cavagna%2C+M%2E%22">Cavagna, M.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Dell’Anna%2C+R%2E%22">Dell’Anna, R.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Malvezzi+Campeggi%2C+F%2E%22">Malvezzi Campeggi, F.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Monti%2C+F%2E%22">Monti, F.</searchLink><relatesTo>1</relatesTo><i> francesca.monti@univr.it</i><br /><searchLink fieldCode="AR" term="%22Rossi%2C+F%2E%22">Rossi, F.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Torriani%2C+S%2E%22">Torriani, S.</searchLink><relatesTo>2</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Vibrational+Spectroscopy%22">Vibrational Spectroscopy</searchLink>. Jul2008, Vol. 47 Issue 2, p139-147. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Fourier+transform+infrared+spectroscopy%22">Fourier transform infrared spectroscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Autolysis%22">Autolysis</searchLink><br /><searchLink fieldCode="DE" term="%22Yeast%22">Yeast</searchLink><br /><searchLink fieldCode="DE" term="%22Saccharomyces+cerevisiae%22">Saccharomyces cerevisiae</searchLink>
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  Data: Abstract: The present paper reports the first application of FTIR microspectroscopy in the mid-infrared range to study the major biochemical changes associated with autolysis in yeast cells. Measurements were done both in transmission and in attenuated total reflection (ATR) mode on cells of Saccharomyces cerevisiae strain EC1118 before and after induction of the autolytic process in a model wine medium and in a Chardonnay base wine. Unsupervised multivariate statistical analysis (Hierarchical Cluster Analysis and Principal Component Analysis), as well as accurate spectral analysis based on curve fitting, were applied to the acquired spectra. The spectral behaviour of S. cerevisiae in the model and base wines was found to be the same. A detailed interpretation of absorption bands was given by reference to the literature and through comparison of transmission and ATR spectra. It was shown that FTIR microspectroscopy is a rapid and accurate tool to simultaneously probe the major biochemical events associated with the autolytic process. Moreover, the intrinsically higher sensitivity of ATR with respect to transmission spectra in analyzing autolysis was also demonstrated. [Copyright &y& Elsevier]
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  Data: <i>Copyright of Vibrational Spectroscopy is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.vibspec.2008.04.007
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        Text: English
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        Type: general
      – SubjectFull: Autolysis
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      – SubjectFull: Yeast
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              Text: Jul2008
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              Y: 2008
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