Molecular insight into activated sludge producing polyhydroxyalkanoates under aerobic–anaerobic conditions.
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| Title: | Molecular insight into activated sludge producing polyhydroxyalkanoates under aerobic–anaerobic conditions. |
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| Authors: | Ciesielski, Slawomir1,2 slavcm@uwm.edu.pl, Pokoj, Tomasz1,2, Klimiuk, Ewa1,2 |
| Source: | Journal of Industrial Microbiology & Biotechnology. Aug2008, Vol. 35 Issue 8, p805-814. 10p. 2 Black and White Photographs, 1 Diagram, 2 Charts, 1 Graph. |
| Subjects: | Poly-beta-hydroxyalkanoates, Sludge bulking, Polymerase chain reaction, Pseudomonas, Biomass, Nucleotide sequence, Gel electrophoresis, Sequencing batch reactor process |
| Abstract: | One of the options enabling more economic production of polyhydroxyalkanoates compared to pure cultures is the application of mixed cultures. The use of a microbial community in a sequencing batch reactor has a few advantages: a simple process control, no necessity for sterile processing, and possibilities of using cheap substrates as a source of carbon. Nevertheless, while cultivation methods to achieve high PHAs biomass concentration and high productivity in wild and recombinant strains are defined, knowledge about the cultivation strategy for PHAs production by mixed culture and species composition of bacterial communities is still very limited. The main object of this study was to characterize on the molecular level the composition and activity of PHAs producing microorganism in activated sludge cultivated under oxygen limitation conditions. PHAs producers were detected using a PCR technique and the created PHA synthase gene library was analyzed by DNA sequencing. The obtained results indicate that PHAs-producers belonged to Pseudomonas sp., and possessed genes coding for mcl-PHA synthase. The kinetics of mcl-PHA synthase expression was relatively estimated using real-time PCR technology at several timepoints. Performed quantitative and qualitative analysis of total bacterial activity showed that there were differences in total activity during the process but differential expression of various groups of microorganisms examined by using DGGE was not observed. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Industrial Microbiology & Biotechnology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 33332615 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Molecular insight into activated sludge producing polyhydroxyalkanoates under aerobic–anaerobic conditions. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Ciesielski%2C+Slawomir%22">Ciesielski, Slawomir</searchLink><relatesTo>1,2</relatesTo><i> slavcm@uwm.edu.pl</i><br /><searchLink fieldCode="AR" term="%22Pokoj%2C+Tomasz%22">Pokoj, Tomasz</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Klimiuk%2C+Ewa%22">Klimiuk, Ewa</searchLink><relatesTo>1,2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Industrial+Microbiology+%26+Biotechnology%22">Journal of Industrial Microbiology & Biotechnology</searchLink>. Aug2008, Vol. 35 Issue 8, p805-814. 10p. 2 Black and White Photographs, 1 Diagram, 2 Charts, 1 Graph. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Poly-beta-hydroxyalkanoates%22">Poly-beta-hydroxyalkanoates</searchLink><br /><searchLink fieldCode="DE" term="%22Sludge+bulking%22">Sludge bulking</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerase+chain+reaction%22">Polymerase chain reaction</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudomonas%22">Pseudomonas</searchLink><br /><searchLink fieldCode="DE" term="%22Biomass%22">Biomass</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleotide+sequence%22">Nucleotide sequence</searchLink><br /><searchLink fieldCode="DE" term="%22Gel+electrophoresis%22">Gel electrophoresis</searchLink><br /><searchLink fieldCode="DE" term="%22Sequencing+batch+reactor+process%22">Sequencing batch reactor process</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: One of the options enabling more economic production of polyhydroxyalkanoates compared to pure cultures is the application of mixed cultures. The use of a microbial community in a sequencing batch reactor has a few advantages: a simple process control, no necessity for sterile processing, and possibilities of using cheap substrates as a source of carbon. Nevertheless, while cultivation methods to achieve high PHAs biomass concentration and high productivity in wild and recombinant strains are defined, knowledge about the cultivation strategy for PHAs production by mixed culture and species composition of bacterial communities is still very limited. The main object of this study was to characterize on the molecular level the composition and activity of PHAs producing microorganism in activated sludge cultivated under oxygen limitation conditions. PHAs producers were detected using a PCR technique and the created PHA synthase gene library was analyzed by DNA sequencing. The obtained results indicate that PHAs-producers belonged to Pseudomonas sp., and possessed genes coding for mcl-PHA synthase. The kinetics of mcl-PHA synthase expression was relatively estimated using real-time PCR technology at several timepoints. Performed quantitative and qualitative analysis of total bacterial activity showed that there were differences in total activity during the process but differential expression of various groups of microorganisms examined by using DGGE was not observed. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Industrial Microbiology & Biotechnology is the property of Oxford University Press / USA and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s10295-008-0352-7 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 10 StartPage: 805 Subjects: – SubjectFull: Poly-beta-hydroxyalkanoates Type: general – SubjectFull: Sludge bulking Type: general – SubjectFull: Polymerase chain reaction Type: general – SubjectFull: Pseudomonas Type: general – SubjectFull: Biomass Type: general – SubjectFull: Nucleotide sequence Type: general – SubjectFull: Gel electrophoresis Type: general – SubjectFull: Sequencing batch reactor process Type: general Titles: – TitleFull: Molecular insight into activated sludge producing polyhydroxyalkanoates under aerobic–anaerobic conditions. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Ciesielski, Slawomir – PersonEntity: Name: NameFull: Pokoj, Tomasz – PersonEntity: Name: NameFull: Klimiuk, Ewa IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2008 Type: published Y: 2008 Identifiers: – Type: issn-print Value: 13675435 Numbering: – Type: volume Value: 35 – Type: issue Value: 8 Titles: – TitleFull: Journal of Industrial Microbiology & Biotechnology Type: main |
| ResultId | 1 |
