Flow cytometry for the evaluation of chromosomal damage in turbot Psetta maxima (L.) exposed to the dissolved fraction of heavy fuel oil in sea water: a comparison with classical biomarkers.
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| Title: | Flow cytometry for the evaluation of chromosomal damage in turbot Psetta maxima (L.) exposed to the dissolved fraction of heavy fuel oil in sea water: a comparison with classical biomarkers. |
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| Authors: | Goanvec, C.1, Theron, M.1 michael.theron@univ-brest.fr, Lacoue-Labarthe, T.1, Poirier, E.1, Guyomarch, J.2, Le-Floch, S.2, Laroche, J.3, Nonnotte, L.1, Nonnotte, G.1 |
| Source: | Journal of Fish Biology. Aug2008, Vol. 73 Issue 2, p395-413. 19p. 1 Diagram, 3 Charts, 5 Graphs. |
| Subjects: | Biological products, Biomarkers, Psetta maxima, Cytochrome P-450 CYP1A1, Enzyme activation |
| Abstract: | Turbot Psetta maxima were exposed 5 days to the dissolved fraction of fuel oil number 2, then decontaminated over 30 days in clean sea water. Biliary metabolites and ethoxyresorufin-O-deethylase (EROD) activity were evaluated during and after the contamination. These results were compared with chromosomal damage measured by flow cytometry (FCM). Erythrocyte nuclear abnormality, micronuclei and immaturity were also evaluated over the exposure period. Biliary metabolites and EROD analyses showed a clear and early response: biliary metabolites were detected from the first day of contamination to the 14th day of depuration, EROD activity increased during the contamination period reached a maximum 3 days after the beginning of the decontamination and decreased to the control value after 1 month of depuration. FCM showed a bimodal response: a first increase of coefficient of variation of blood cell DNA content was observed during the contamination and a second one started after 14 days of depuration and was maintained for at least 2 weeks. Erythrocyte morphology analysis showed a strong increase in nuclear abnormality during the contamination period. These results confirm previous work and show that in the context of marine accidental pollution by heavy fuel oil, the measurements of chromosomal damage by FCM allow the detection of a genotoxic response in fishes. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Fish Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 33377896 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Flow cytometry for the evaluation of chromosomal damage in turbot Psetta maxima (L.) exposed to the dissolved fraction of heavy fuel oil in sea water: a comparison with classical biomarkers. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Goanvec%2C+C%2E%22">Goanvec, C.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Theron%2C+M%2E%22">Theron, M.</searchLink><relatesTo>1</relatesTo><i> michael.theron@univ-brest.fr</i><br /><searchLink fieldCode="AR" term="%22Lacoue-Labarthe%2C+T%2E%22">Lacoue-Labarthe, T.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Poirier%2C+E%2E%22">Poirier, E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Guyomarch%2C+J%2E%22">Guyomarch, J.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Le-Floch%2C+S%2E%22">Le-Floch, S.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Laroche%2C+J%2E%22">Laroche, J.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Nonnotte%2C+L%2E%22">Nonnotte, L.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Nonnotte%2C+G%2E%22">Nonnotte, G.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Fish+Biology%22">Journal of Fish Biology</searchLink>. Aug2008, Vol. 73 Issue 2, p395-413. 19p. 1 Diagram, 3 Charts, 5 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Biological+products%22">Biological products</searchLink><br /><searchLink fieldCode="DE" term="%22Biomarkers%22">Biomarkers</searchLink><br /><searchLink fieldCode="DE" term="%22Psetta+maxima%22">Psetta maxima</searchLink><br /><searchLink fieldCode="DE" term="%22Cytochrome+P-450+CYP1A1%22">Cytochrome P-450 CYP1A1</searchLink><br /><searchLink fieldCode="DE" term="%22Enzyme+activation%22">Enzyme activation</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Turbot Psetta maxima were exposed 5 days to the dissolved fraction of fuel oil number 2, then decontaminated over 30 days in clean sea water. Biliary metabolites and ethoxyresorufin-O-deethylase (EROD) activity were evaluated during and after the contamination. These results were compared with chromosomal damage measured by flow cytometry (FCM). Erythrocyte nuclear abnormality, micronuclei and immaturity were also evaluated over the exposure period. Biliary metabolites and EROD analyses showed a clear and early response: biliary metabolites were detected from the first day of contamination to the 14th day of depuration, EROD activity increased during the contamination period reached a maximum 3 days after the beginning of the decontamination and decreased to the control value after 1 month of depuration. FCM showed a bimodal response: a first increase of coefficient of variation of blood cell DNA content was observed during the contamination and a second one started after 14 days of depuration and was maintained for at least 2 weeks. Erythrocyte morphology analysis showed a strong increase in nuclear abnormality during the contamination period. These results confirm previous work and show that in the context of marine accidental pollution by heavy fuel oil, the measurements of chromosomal damage by FCM allow the detection of a genotoxic response in fishes. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Fish Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/j.1095-8649.2008.01901.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 19 StartPage: 395 Subjects: – SubjectFull: Biological products Type: general – SubjectFull: Biomarkers Type: general – SubjectFull: Psetta maxima Type: general – SubjectFull: Cytochrome P-450 CYP1A1 Type: general – SubjectFull: Enzyme activation Type: general Titles: – TitleFull: Flow cytometry for the evaluation of chromosomal damage in turbot Psetta maxima (L.) exposed to the dissolved fraction of heavy fuel oil in sea water: a comparison with classical biomarkers. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Goanvec, C. – PersonEntity: Name: NameFull: Theron, M. – PersonEntity: Name: NameFull: Lacoue-Labarthe, T. – PersonEntity: Name: NameFull: Poirier, E. – PersonEntity: Name: NameFull: Guyomarch, J. – PersonEntity: Name: NameFull: Le-Floch, S. – PersonEntity: Name: NameFull: Laroche, J. – PersonEntity: Name: NameFull: Nonnotte, L. – PersonEntity: Name: NameFull: Nonnotte, G. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2008 Type: published Y: 2008 Identifiers: – Type: issn-print Value: 00221112 Numbering: – Type: volume Value: 73 – Type: issue Value: 2 Titles: – TitleFull: Journal of Fish Biology Type: main |
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