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Contribution of Individual Amino Acids to the 5S RNA Binding Activity of the Xenopus Zinc Finger Protein p43.

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Title: Contribution of Individual Amino Acids to the 5S RNA Binding Activity of the Xenopus Zinc Finger Protein p43.
Authors: Simran S. Bhatia1, Tristen C. Weiss1, Paul J. Romaniuk1 pjr@uvic.ca
Source: Biochemistry. 8/12/2008, Vol. 47 Issue 32, p8398-8405. 8p. 2 Diagrams, 2 Charts, 3 Graphs.
Subjects: Xenopus, Zinc, Nucleoproteins, RNA, Amino acids
Abstract: Xenopus zinc finger protein p43 binds to 5S RNA in immature oocytes to form a 42S ribonucleoprotein storage particle. To determine the role of individual zinc fingers of the protein in this RNA binding activity, a series of deletion and substitution mutants of p43 were constructed. The effects of the various mutations on the RNA binding activity of p43 were determined using a quantitative equilibrium binding assay. The results indicate that zinc fingers 1 and 4 of p43 are essential for the binding of the protein to 5S RNA. In the case of finger 1, four amino acids key to RNA binding are found on the same face of the α-helix, while in the case of finger 4, two key residues are clustered at the start of the α-helix. The similarities and differences in the mechanisms by which fingers 1 and 4 of p43 interact with 5S RNA are compared to the interaction of the zinc fingers of Xenopus transcription factor IIIA with 5S RNA. [ABSTRACT FROM AUTHOR]
Copyright of Biochemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 34010827
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PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
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  Data: Contribution of Individual Amino Acids to the 5S RNA Binding Activity of the Xenopus Zinc Finger Protein p43.
– Name: Author
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  Data: <searchLink fieldCode="AR" term="%22Simran+S%2E+Bhatia%22">Simran S. Bhatia</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Tristen+C%2E+Weiss%22">Tristen C. Weiss</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Paul+J%2E+Romaniuk%22">Paul J. Romaniuk</searchLink><relatesTo>1</relatesTo><i> pjr@uvic.ca</i>
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  Data: <searchLink fieldCode="JN" term="%22Biochemistry%22">Biochemistry</searchLink>. 8/12/2008, Vol. 47 Issue 32, p8398-8405. 8p. 2 Diagrams, 2 Charts, 3 Graphs.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Xenopus%22">Xenopus</searchLink><br /><searchLink fieldCode="DE" term="%22Zinc%22">Zinc</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleoproteins%22">Nucleoproteins</searchLink><br /><searchLink fieldCode="DE" term="%22RNA%22">RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Amino+acids%22">Amino acids</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Xenopus zinc finger protein p43 binds to 5S RNA in immature oocytes to form a 42S ribonucleoprotein storage particle. To determine the role of individual zinc fingers of the protein in this RNA binding activity, a series of deletion and substitution mutants of p43 were constructed. The effects of the various mutations on the RNA binding activity of p43 were determined using a quantitative equilibrium binding assay. The results indicate that zinc fingers 1 and 4 of p43 are essential for the binding of the protein to 5S RNA. In the case of finger 1, four amino acids key to RNA binding are found on the same face of the α-helix, while in the case of finger 4, two key residues are clustered at the start of the α-helix. The similarities and differences in the mechanisms by which fingers 1 and 4 of p43 interact with 5S RNA are compared to the interaction of the zinc fingers of Xenopus transcription factor IIIA with 5S RNA. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biochemistry is the property of American Chemical Society and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1021/bi800080c
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      – Code: eng
        Text: English
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        PageCount: 8
        StartPage: 8398
    Subjects:
      – SubjectFull: Xenopus
        Type: general
      – SubjectFull: Zinc
        Type: general
      – SubjectFull: Nucleoproteins
        Type: general
      – SubjectFull: RNA
        Type: general
      – SubjectFull: Amino acids
        Type: general
    Titles:
      – TitleFull: Contribution of Individual Amino Acids to the 5S RNA Binding Activity of the Xenopus Zinc Finger Protein p43.
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            NameFull: Simran S. Bhatia
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            NameFull: Tristen C. Weiss
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            NameFull: Paul J. Romaniuk
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              M: 08
              Text: 8/12/2008
              Type: published
              Y: 2008
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              Value: 47
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              Value: 32
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            – TitleFull: Biochemistry
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