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Unmarked insertional mutagenesis in the bovine pathogen Mycoplasma mycoides subsp. mycoides SC: characterization of a IppQ mutant.

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Title: Unmarked insertional mutagenesis in the bovine pathogen Mycoplasma mycoides subsp. mycoides SC: characterization of a IppQ mutant.
Authors: Janis, Carole1,2, Bischof, Daniela3, Gourgues, Geraldine1,2, Frey, Joachim3, Blanchard, Alain1,2, Sirand-Pugnet, Pascal1,2 sirand@bordeaux.inra.fr
Source: Microbiology (13500872). Aug2008, Vol. 154 Issue 8, p2427-2436. 10p. 1 Diagram, 1 Graph.
Subjects: Mycoplasma, Mutagenesis, Transposons, Mobile genetic elements, Genes, Genetic mutation, Operons, Genetic transcription, Microbiology
Abstract: The article reports on a study which develops a transposon-based approach for the random mutagenesis of Mycoplasma mycoides subsp. mycoides small colony (SC). According to the authors of the study, transposon insertion sites were screened using a PCR-based method to evaluate the diversity of mutants within the library. The three loci with genes involved in the pathogenicity of M. mycoides subsp. mycoides SC were search, including gtsABS operon, glpO gene and the lppQ gene. The study showed that the mutant was found to express only residual amounts of the truncated N-terminal end of lppQ.
Database: Engineering Source
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DbLabel: Engineering Source
An: 34224609
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Unmarked insertional mutagenesis in the bovine pathogen Mycoplasma mycoides subsp. mycoides SC: characterization of a IppQ mutant.
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  Data: <searchLink fieldCode="AR" term="%22Janis%2C+Carole%22">Janis, Carole</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Bischof%2C+Daniela%22">Bischof, Daniela</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Gourgues%2C+Geraldine%22">Gourgues, Geraldine</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Frey%2C+Joachim%22">Frey, Joachim</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Blanchard%2C+Alain%22">Blanchard, Alain</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Sirand-Pugnet%2C+Pascal%22">Sirand-Pugnet, Pascal</searchLink><relatesTo>1,2</relatesTo><i> sirand@bordeaux.inra.fr</i>
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  Data: <searchLink fieldCode="JN" term="%22Microbiology+%2813500872%29%22">Microbiology (13500872)</searchLink>. Aug2008, Vol. 154 Issue 8, p2427-2436. 10p. 1 Diagram, 1 Graph.
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  Data: <searchLink fieldCode="DE" term="%22Mycoplasma%22">Mycoplasma</searchLink><br /><searchLink fieldCode="DE" term="%22Mutagenesis%22">Mutagenesis</searchLink><br /><searchLink fieldCode="DE" term="%22Transposons%22">Transposons</searchLink><br /><searchLink fieldCode="DE" term="%22Mobile+genetic+elements%22">Mobile genetic elements</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+mutation%22">Genetic mutation</searchLink><br /><searchLink fieldCode="DE" term="%22Operons%22">Operons</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+transcription%22">Genetic transcription</searchLink><br /><searchLink fieldCode="DE" term="%22Microbiology%22">Microbiology</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: The article reports on a study which develops a transposon-based approach for the random mutagenesis of Mycoplasma mycoides subsp. mycoides small colony (SC). According to the authors of the study, transposon insertion sites were screened using a PCR-based method to evaluate the diversity of mutants within the library. The three loci with genes involved in the pathogenicity of M. mycoides subsp. mycoides SC were search, including gtsABS operon, glpO gene and the lppQ gene. The study showed that the mutant was found to express only residual amounts of the truncated N-terminal end of lppQ.
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=34224609
RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1099/mic.0.2008/017640-0
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 10
        StartPage: 2427
    Subjects:
      – SubjectFull: Mycoplasma
        Type: general
      – SubjectFull: Mutagenesis
        Type: general
      – SubjectFull: Transposons
        Type: general
      – SubjectFull: Mobile genetic elements
        Type: general
      – SubjectFull: Genes
        Type: general
      – SubjectFull: Genetic mutation
        Type: general
      – SubjectFull: Operons
        Type: general
      – SubjectFull: Genetic transcription
        Type: general
      – SubjectFull: Microbiology
        Type: general
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      – TitleFull: Unmarked insertional mutagenesis in the bovine pathogen Mycoplasma mycoides subsp. mycoides SC: characterization of a IppQ mutant.
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            NameFull: Janis, Carole
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            NameFull: Bischof, Daniela
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            NameFull: Gourgues, Geraldine
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            NameFull: Frey, Joachim
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            NameFull: Blanchard, Alain
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            NameFull: Sirand-Pugnet, Pascal
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            – D: 01
              M: 08
              Text: Aug2008
              Type: published
              Y: 2008
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              Value: 13500872
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              Value: 154
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              Value: 8
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            – TitleFull: Microbiology (13500872)
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