A Multilayer MEMS Platform for Single-Cell Electric Impedance Spectroscopy and Electrochemical Analysis.
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| Title: | A Multilayer MEMS Platform for Single-Cell Electric Impedance Spectroscopy and Electrochemical Analysis. |
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| Authors: | Dittami, Gregory M.1 Dittami@eng.utah.edu, Ayliffe, H. Edward2, King, Curtis S.2, Rabbitt, Richard D.1 |
| Source: | Journal of Microelectromechanical Systems. Aug2008, Vol. 17 Issue 4, p850-862. 13p. 15 Diagrams. |
| Subjects: | Microelectromechanical systems, Radioactive waste characterization, Hair cells, Microfluidics, Cochlea, Biochemical genetics, Interface circuits, Fluidic devices, Impedance spectroscopy |
| Abstract: | The fabrication and characterization of a microchamber electrode array for electrical and electrochemical studies of individual biological cells are presented. The geometry was tailored specifically for measurements from sensory hair cells isolated from the cochlea of the mammalian inner ear. Conventional microelectromechanical system (MEMS) fabrication techniques were combined with a heat-sealing technique and polydimethylsiloxane micromolding to achieve a multilayered microfluidic system that facilitates cell manipulation and selection. The system allowed for electrical stimulation of individual living cells and interrogation of excitable cell membrane dielectric properties as a function of space and time. A three-electrode impedimetric system was incorporated to provide the additional ability to record the time-dependent concentrations of specific biochemicals in microdomain volumes near identified regions of the cell membrane. The design and fabrication of a robust fluidic and electrical interface are also described. The interface provided the flexibility and simplicity of a "cartridge- based" approach in connecting to the MEMS devices. Cytometric measurement capabilities were characterized by using electric impedance spectroscopy (1 kHz-10 MHz) of isolated outer hair cells. Chemical sensing capability within the microchannel recording chamber was characterized by using cyclic voltammetry with varying concentrations of potassium ferricyanide (K3Fe(CN)6). Chronoamperometric recordings of electrically stimulated PC12 cells highlight the ability of the platform to resolve exocytosis events from individual cells. [ABSTRACT FROM AUTHOR] |
| Copyright of Journal of Microelectromechanical Systems is the property of IEEE and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 34260193 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: A Multilayer MEMS Platform for Single-Cell Electric Impedance Spectroscopy and Electrochemical Analysis. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dittami%2C+Gregory+M%2E%22">Dittami, Gregory M.</searchLink><relatesTo>1</relatesTo><i> Dittami@eng.utah.edu</i><br /><searchLink fieldCode="AR" term="%22Ayliffe%2C+H%2E+Edward%22">Ayliffe, H. Edward</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22King%2C+Curtis+S%2E%22">King, Curtis S.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Rabbitt%2C+Richard+D%2E%22">Rabbitt, Richard D.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Microelectromechanical+Systems%22">Journal of Microelectromechanical Systems</searchLink>. Aug2008, Vol. 17 Issue 4, p850-862. 13p. 15 Diagrams. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Microelectromechanical+systems%22">Microelectromechanical systems</searchLink><br /><searchLink fieldCode="DE" term="%22Radioactive+waste+characterization%22">Radioactive waste characterization</searchLink><br /><searchLink fieldCode="DE" term="%22Hair+cells%22">Hair cells</searchLink><br /><searchLink fieldCode="DE" term="%22Microfluidics%22">Microfluidics</searchLink><br /><searchLink fieldCode="DE" term="%22Cochlea%22">Cochlea</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemical+genetics%22">Biochemical genetics</searchLink><br /><searchLink fieldCode="DE" term="%22Interface+circuits%22">Interface circuits</searchLink><br /><searchLink fieldCode="DE" term="%22Fluidic+devices%22">Fluidic devices</searchLink><br /><searchLink fieldCode="DE" term="%22Impedance+spectroscopy%22">Impedance spectroscopy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: The fabrication and characterization of a microchamber electrode array for electrical and electrochemical studies of individual biological cells are presented. The geometry was tailored specifically for measurements from sensory hair cells isolated from the cochlea of the mammalian inner ear. Conventional microelectromechanical system (MEMS) fabrication techniques were combined with a heat-sealing technique and polydimethylsiloxane micromolding to achieve a multilayered microfluidic system that facilitates cell manipulation and selection. The system allowed for electrical stimulation of individual living cells and interrogation of excitable cell membrane dielectric properties as a function of space and time. A three-electrode impedimetric system was incorporated to provide the additional ability to record the time-dependent concentrations of specific biochemicals in microdomain volumes near identified regions of the cell membrane. The design and fabrication of a robust fluidic and electrical interface are also described. The interface provided the flexibility and simplicity of a "cartridge- based" approach in connecting to the MEMS devices. Cytometric measurement capabilities were characterized by using electric impedance spectroscopy (1 kHz-10 MHz) of isolated outer hair cells. Chemical sensing capability within the microchannel recording chamber was characterized by using cyclic voltammetry with varying concentrations of potassium ferricyanide (K3Fe(CN)6). Chronoamperometric recordings of electrically stimulated PC12 cells highlight the ability of the platform to resolve exocytosis events from individual cells. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Microelectromechanical Systems is the property of IEEE and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1109/JMEMS.2008.921726 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 850 Subjects: – SubjectFull: Microelectromechanical systems Type: general – SubjectFull: Radioactive waste characterization Type: general – SubjectFull: Hair cells Type: general – SubjectFull: Microfluidics Type: general – SubjectFull: Cochlea Type: general – SubjectFull: Biochemical genetics Type: general – SubjectFull: Interface circuits Type: general – SubjectFull: Fluidic devices Type: general – SubjectFull: Impedance spectroscopy Type: general Titles: – TitleFull: A Multilayer MEMS Platform for Single-Cell Electric Impedance Spectroscopy and Electrochemical Analysis. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dittami, Gregory M. – PersonEntity: Name: NameFull: Ayliffe, H. Edward – PersonEntity: Name: NameFull: King, Curtis S. – PersonEntity: Name: NameFull: Rabbitt, Richard D. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2008 Type: published Y: 2008 Identifiers: – Type: issn-print Value: 10577157 Numbering: – Type: volume Value: 17 – Type: issue Value: 4 Titles: – TitleFull: Journal of Microelectromechanical Systems Type: main |
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