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Regulation of Cyst Wall Protein Promoters by Myb2 in Giardia lamblia.

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Title: Regulation of Cyst Wall Protein Promoters by Myb2 in Giardia lamblia.
Authors: Yu-Chang Huang1, Li-Hsin Su1, Lee, Gilbert A.1, Pei-Wei Chiu1, Chao-Cheng Cho1, Jeng-You Wu1, Chin-Hung Sun1 chinhsun@ntu.edu.tw
Source: Journal of Biological Chemistry. 11/7/2008, Vol. 283 Issue 45, p31021-31029. 9p. 7 Diagrams, 1 Graph.
Subjects: Giardia lamblia, Transcription factors, Cell proliferation, Cell cycle, Gene expression, Chromatin, Biochemistry
Abstract: Myb family transcription factors are important in regulating cell proliferation, differentiation, and cell cycle progression. Giardia lamblia differentiates into infectious cysts to survive outside of the host. During encystation, genes encoding cyst wall proteins (CWPs) are coordinately induced. We have identified an encystation-induced Myb2 protein, which binds to the promoter regions of the cwp genes and myb2 itself in vitro. To elucidate the role of Myb2 in G. lamblia, we tested the hypothesis that Myb2 can activate encystation-induced genes. We found that overexpression of Myb2 resulted in an increase of expression of CWP1 at both protein and mRNA levels. Interestingly, the Myb2-overexpressing trophozoites had increased capability to differentiate into cysts. In cotransfection assays, Myb2 was able to transactivate the cwp promoters and its own promoter in vivo, suggesting that its gene can be positively autoregulated. Moreover, deletion of the N- or C-terminal domain resulted in a decrease of transactivation and autoregulation function of Myb2. We also found that the promoter of a newly identified encystation-induced gene, the giardial myeloid leukemia factor-like gene, has the Myb2 binding sites and that its mRNA levels were increased by Myb2 overexpression. Chromatin immunoprecipitation assays confirmed that Myb2 was bound to the promoters with its binding sites. Transfection of the myb2 antisense construct reduced the levels of the cwp1 transcripts and cyst formation. Our results suggest that Myb2 is a potent transactivator of the cwp genes and other endogenous genes and plays an important role in G. lamblia differentiation into cysts. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Regulation of Cyst Wall Protein Promoters by Myb2 in Giardia lamblia.
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  Data: <searchLink fieldCode="AR" term="%22Yu-Chang+Huang%22">Yu-Chang Huang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Li-Hsin+Su%22">Li-Hsin Su</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lee%2C+Gilbert+A%2E%22">Lee, Gilbert A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Pei-Wei+Chiu%22">Pei-Wei Chiu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chao-Cheng+Cho%22">Chao-Cheng Cho</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Jeng-You+Wu%22">Jeng-You Wu</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chin-Hung+Sun%22">Chin-Hung Sun</searchLink><relatesTo>1</relatesTo><i> chinhsun@ntu.edu.tw</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Biological+Chemistry%22">Journal of Biological Chemistry</searchLink>. 11/7/2008, Vol. 283 Issue 45, p31021-31029. 9p. 7 Diagrams, 1 Graph.
– Name: Subject
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  Data: <searchLink fieldCode="DE" term="%22Giardia+lamblia%22">Giardia lamblia</searchLink><br /><searchLink fieldCode="DE" term="%22Transcription+factors%22">Transcription factors</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+cycle%22">Cell cycle</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Chromatin%22">Chromatin</searchLink><br /><searchLink fieldCode="DE" term="%22Biochemistry%22">Biochemistry</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Myb family transcription factors are important in regulating cell proliferation, differentiation, and cell cycle progression. Giardia lamblia differentiates into infectious cysts to survive outside of the host. During encystation, genes encoding cyst wall proteins (CWPs) are coordinately induced. We have identified an encystation-induced Myb2 protein, which binds to the promoter regions of the cwp genes and myb2 itself in vitro. To elucidate the role of Myb2 in G. lamblia, we tested the hypothesis that Myb2 can activate encystation-induced genes. We found that overexpression of Myb2 resulted in an increase of expression of CWP1 at both protein and mRNA levels. Interestingly, the Myb2-overexpressing trophozoites had increased capability to differentiate into cysts. In cotransfection assays, Myb2 was able to transactivate the cwp promoters and its own promoter in vivo, suggesting that its gene can be positively autoregulated. Moreover, deletion of the N- or C-terminal domain resulted in a decrease of transactivation and autoregulation function of Myb2. We also found that the promoter of a newly identified encystation-induced gene, the giardial myeloid leukemia factor-like gene, has the Myb2 binding sites and that its mRNA levels were increased by Myb2 overexpression. Chromatin immunoprecipitation assays confirmed that Myb2 was bound to the promoters with its binding sites. Transfection of the myb2 antisense construct reduced the levels of the cwp1 transcripts and cyst formation. Our results suggest that Myb2 is a potent transactivator of the cwp genes and other endogenous genes and plays an important role in G. lamblia differentiation into cysts. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Biological Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1074/jbc.M805023200
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 9
        StartPage: 31021
    Subjects:
      – SubjectFull: Giardia lamblia
        Type: general
      – SubjectFull: Transcription factors
        Type: general
      – SubjectFull: Cell proliferation
        Type: general
      – SubjectFull: Cell cycle
        Type: general
      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: Chromatin
        Type: general
      – SubjectFull: Biochemistry
        Type: general
    Titles:
      – TitleFull: Regulation of Cyst Wall Protein Promoters by Myb2 in Giardia lamblia.
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            NameFull: Yu-Chang Huang
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            NameFull: Li-Hsin Su
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            NameFull: Lee, Gilbert A.
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            NameFull: Chao-Cheng Cho
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            NameFull: Chin-Hung Sun
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              M: 11
              Text: 11/7/2008
              Type: published
              Y: 2008
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              Value: 283
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              Value: 45
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