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The importance of the effect of shear stress on endothelial cells in determining the performance of hemoglobin based oxygen carriers

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Title: The importance of the effect of shear stress on endothelial cells in determining the performance of hemoglobin based oxygen carriers
Authors: Gaucher-Di Stasio, Caroline1, Paternotte, Estelle1, Prin-Mathieu, Christine2, Reeder, Brandon J.3, Poitevin, Gaël4, Labrude, Pierre1, Stoltz, Jean-François1, Cooper, Chris E.3, Menu, Patrick1 patrick.menu@medecine.uhp-nancy.fr
Source: Biomaterials. Feb2009, Vol. 30 Issue 4, p445-451. 7p.
Subjects: Oxygen carriers, Hemoglobins, Cellular mechanics, Polyethylene glycol, Nitric oxide, Endothelium, Vasoconstriction, Reactive oxygen species
Abstract: Abstract: The lack of blood donations and the threat of infections from blood and blood products have led to extensive research into the development of blood substitutes. The latest generation of hemoglobin based oxygen carriers (HBOC) has been shown to induce side effects like hypertension, vasoconstriction, inflammation and oxidative stress. HBOC are able to restore volemia and transport oxygen after a hemorrhagic shock, the reperfusion leading to the restoration of the blood flow in vessels. We propose an innovative approach, more closely emulating clinical situations, to assess the impact of HBOC perfusion on endothelial cells (EC) in vitro. Through this approach we quantified levels of oxidative stress, vasoactive factors and inflammation. EC were cultivated under a laminar flow to reproduce the return of shear stress (SS) during the reperfusion. We showed that heme oxygenase I transcription correlated with changes in oxidatively modified heme and methemoglobin; all were lower under SS. SS induced increased nitric oxide production, which may have implications for the mechanism of in vivo vasoconstriction and hypertension. E-selectin changes under SS were greater than those of ICAM-1. Our results demonstrate how it is essential to include SS in assays attempting to understand the potential vascular side effects of HBOC perfusion. [Copyright &y& Elsevier]
Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 35505556
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: The importance of the effect of shear stress on endothelial cells in determining the performance of hemoglobin based oxygen carriers
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  Data: <searchLink fieldCode="AR" term="%22Gaucher-Di+Stasio%2C+Caroline%22">Gaucher-Di Stasio, Caroline</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Paternotte%2C+Estelle%22">Paternotte, Estelle</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Prin-Mathieu%2C+Christine%22">Prin-Mathieu, Christine</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Reeder%2C+Brandon+J%2E%22">Reeder, Brandon J.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Poitevin%2C+Gaël%22">Poitevin, Gaël</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Labrude%2C+Pierre%22">Labrude, Pierre</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Stoltz%2C+Jean-François%22">Stoltz, Jean-François</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cooper%2C+Chris+E%2E%22">Cooper, Chris E.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Menu%2C+Patrick%22">Menu, Patrick</searchLink><relatesTo>1</relatesTo><i> patrick.menu@medecine.uhp-nancy.fr</i>
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  Data: <searchLink fieldCode="JN" term="%22Biomaterials%22">Biomaterials</searchLink>. Feb2009, Vol. 30 Issue 4, p445-451. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Oxygen+carriers%22">Oxygen carriers</searchLink><br /><searchLink fieldCode="DE" term="%22Hemoglobins%22">Hemoglobins</searchLink><br /><searchLink fieldCode="DE" term="%22Cellular+mechanics%22">Cellular mechanics</searchLink><br /><searchLink fieldCode="DE" term="%22Polyethylene+glycol%22">Polyethylene glycol</searchLink><br /><searchLink fieldCode="DE" term="%22Nitric+oxide%22">Nitric oxide</searchLink><br /><searchLink fieldCode="DE" term="%22Endothelium%22">Endothelium</searchLink><br /><searchLink fieldCode="DE" term="%22Vasoconstriction%22">Vasoconstriction</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink>
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  Data: Abstract: The lack of blood donations and the threat of infections from blood and blood products have led to extensive research into the development of blood substitutes. The latest generation of hemoglobin based oxygen carriers (HBOC) has been shown to induce side effects like hypertension, vasoconstriction, inflammation and oxidative stress. HBOC are able to restore volemia and transport oxygen after a hemorrhagic shock, the reperfusion leading to the restoration of the blood flow in vessels. We propose an innovative approach, more closely emulating clinical situations, to assess the impact of HBOC perfusion on endothelial cells (EC) in vitro. Through this approach we quantified levels of oxidative stress, vasoactive factors and inflammation. EC were cultivated under a laminar flow to reproduce the return of shear stress (SS) during the reperfusion. We showed that heme oxygenase I transcription correlated with changes in oxidatively modified heme and methemoglobin; all were lower under SS. SS induced increased nitric oxide production, which may have implications for the mechanism of in vivo vasoconstriction and hypertension. E-selectin changes under SS were greater than those of ICAM-1. Our results demonstrate how it is essential to include SS in assays attempting to understand the potential vascular side effects of HBOC perfusion. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.biomaterials.2008.09.051
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        Text: English
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      – SubjectFull: Oxygen carriers
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      – SubjectFull: Hemoglobins
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      – SubjectFull: Cellular mechanics
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      – SubjectFull: Polyethylene glycol
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      – SubjectFull: Vasoconstriction
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      – SubjectFull: Reactive oxygen species
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              Text: Feb2009
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