Microarray analysis of vegetative phase change in maize.
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| Title: | Microarray analysis of vegetative phase change in maize. |
|---|---|
| Authors: | Strable, Josh1,2, Borsuk, Lisa3, Nettleton, Dan4, Schnable, Patrick S.3, Irish, Erin E.1 erin-irish@uiowa.edu |
| Source: | Plant Journal. Dec2008, Vol. 56 Issue 6, p1045-1057. 13p. 2 Diagrams, 2 Charts, 4 Graphs. |
| Subjects: | Corn, DNA microarrays, Genetic regulation, Plant cells & tissues, Gene expression, Plant growth, Phase transitions |
| Abstract: | Vegetative phase change is the developmental transition from the juvenile phase to the adult phase in which a plant becomes competent for sexual reproduction. The gain of ability to flower is often accompanied by changes in patterns of differentiation in newly forming vegetative organs. In maize, juvenile leaves differ from adult leaves in morphology, anatomy and cell wall composition. Whereas the normal sequence of juvenile followed by adult is repeated with every sexual generation, this sequence can be altered in maize by the isolation and culture of the shoot apex from an adult phase plant: an ‘adult’ meristem so treated reverts to forming juvenile vegetative organs. To begin to unravel the as-yet poorly understood molecular mechanisms underlying phase change in maize, we compared gene expression in two juvenile sample types, leaf 4 and culture-derived leaves 3 or 4, with an adult sample type (leaf 9) using cDNA microarrays. All samples were leaf primordia at plastochron 6. A gene was scored as ‘phase induced’ if it was up- or downregulated in both juvenile sample types, compared with the adult sample type, with at least a twofold change in gene expression at a P-value of ≤0.005. Some 221 expressed sequence tags (ESTs) were upregulated in juveniles, and 28 ESTs were upregulated in adults. The largest class of juvenile-induced genes was comprised of those involved in photosynthesis, suggesting that maize plants are primed for energy production early in vegetative growth by the developmental induction of photosynthetic genes. [ABSTRACT FROM AUTHOR] |
| Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 35604397 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Microarray analysis of vegetative phase change in maize. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Strable%2C+Josh%22">Strable, Josh</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Borsuk%2C+Lisa%22">Borsuk, Lisa</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Nettleton%2C+Dan%22">Nettleton, Dan</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Schnable%2C+Patrick+S%2E%22">Schnable, Patrick S.</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Irish%2C+Erin+E%2E%22">Irish, Erin E.</searchLink><relatesTo>1</relatesTo><i> erin-irish@uiowa.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Plant+Journal%22">Plant Journal</searchLink>. Dec2008, Vol. 56 Issue 6, p1045-1057. 13p. 2 Diagrams, 2 Charts, 4 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Corn%22">Corn</searchLink><br /><searchLink fieldCode="DE" term="%22DNA+microarrays%22">DNA microarrays</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+regulation%22">Genetic regulation</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+cells+%26+tissues%22">Plant cells & tissues</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink><br /><searchLink fieldCode="DE" term="%22Plant+growth%22">Plant growth</searchLink><br /><searchLink fieldCode="DE" term="%22Phase+transitions%22">Phase transitions</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Vegetative phase change is the developmental transition from the juvenile phase to the adult phase in which a plant becomes competent for sexual reproduction. The gain of ability to flower is often accompanied by changes in patterns of differentiation in newly forming vegetative organs. In maize, juvenile leaves differ from adult leaves in morphology, anatomy and cell wall composition. Whereas the normal sequence of juvenile followed by adult is repeated with every sexual generation, this sequence can be altered in maize by the isolation and culture of the shoot apex from an adult phase plant: an ‘adult’ meristem so treated reverts to forming juvenile vegetative organs. To begin to unravel the as-yet poorly understood molecular mechanisms underlying phase change in maize, we compared gene expression in two juvenile sample types, leaf 4 and culture-derived leaves 3 or 4, with an adult sample type (leaf 9) using cDNA microarrays. All samples were leaf primordia at plastochron 6. A gene was scored as ‘phase induced’ if it was up- or downregulated in both juvenile sample types, compared with the adult sample type, with at least a twofold change in gene expression at a P-value of ≤0.005. Some 221 expressed sequence tags (ESTs) were upregulated in juveniles, and 28 ESTs were upregulated in adults. The largest class of juvenile-induced genes was comprised of those involved in photosynthesis, suggesting that maize plants are primed for energy production early in vegetative growth by the developmental induction of photosynthetic genes. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/j.1365-313X.2008.03661.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 13 StartPage: 1045 Subjects: – SubjectFull: Corn Type: general – SubjectFull: DNA microarrays Type: general – SubjectFull: Genetic regulation Type: general – SubjectFull: Plant cells & tissues Type: general – SubjectFull: Gene expression Type: general – SubjectFull: Plant growth Type: general – SubjectFull: Phase transitions Type: general Titles: – TitleFull: Microarray analysis of vegetative phase change in maize. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Strable, Josh – PersonEntity: Name: NameFull: Borsuk, Lisa – PersonEntity: Name: NameFull: Nettleton, Dan – PersonEntity: Name: NameFull: Schnable, Patrick S. – PersonEntity: Name: NameFull: Irish, Erin E. IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 12 Text: Dec2008 Type: published Y: 2008 Identifiers: – Type: issn-print Value: 09607412 Numbering: – Type: volume Value: 56 – Type: issue Value: 6 Titles: – TitleFull: Plant Journal Type: main |
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