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PAMP-induced defense responses in potato require both salicylic acid and jasmonic acid.

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Title: PAMP-induced defense responses in potato require both salicylic acid and jasmonic acid.
Authors: Halim, Vincentius A.1, Altmann, Simone1, Ellinger, Dorothea1, Eschen-Lippold, Lennart1, Miersch, Otto2, Scheel, Dierk1, Rosahl, Sabine1 srosahl@ipb-halle.de
Source: Plant Journal. Jan2009, Vol. 57 Issue 2, p230-242. 13p. 2 Diagrams, 5 Graphs.
Subjects: RNA, Nucleic acids, Heredity, Cell death, Cells, Genes, Reactive oxygen species, Photosynthetic oxygen evolution, Disinfection & disinfectants, Salicylic acid, Transgenic organisms
Abstract: To elucidate the molecular mechanisms underlying pathogen-associated molecular pattern (PAMP)-induced defense responses in potato ( Solanum tuberosum), the role of the signaling compounds salicylic acid (SA) and jasmonic acid (JA) was analyzed. Pep-13, a PAMP from Phytophthora, induces the accumulation of SA, JA and hydrogen peroxide, as well as the activation of defense genes and hypersensitive-like cell death. We have previously shown that SA is required for Pep-13-induced defense responses. To assess the importance of JA, RNA interference constructs targeted at the JA biosynthetic genes, allene oxide cyclase and 12-oxophytodienoic acid reductase, were expressed in transgenic potato plants. In addition, expression of the F-box protein COI1 was reduced by RNA interference. Plants expressing the RNA interference constructs failed to accumulate the respective transcripts in response to wounding or Pep-13 treatment, neither did they contain significant amounts of JA after elicitation. In response to infiltration of Pep-13, the transgenic plants exhibited a highly reduced accumulation of reactive oxygen species as well as reduced hypersensitive cell death. The ability of the JA-deficient plants to accumulate SA suggests that SA accumulation is independent or upstream of JA accumulation. These data show that PAMP responses in potato require both SA and JA and that, in contrast to Arabidopsis, these compounds act in the same signal transduction pathway. Despite their inability to fully respond to PAMP treatment, the transgenic RNA interference plants are not altered in their basal defense against Phytophthora infestans. [ABSTRACT FROM AUTHOR]
Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: PAMP-induced defense responses in potato require both salicylic acid and jasmonic acid.
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  Data: <searchLink fieldCode="AR" term="%22Halim%2C+Vincentius+A%2E%22">Halim, Vincentius A.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Altmann%2C+Simone%22">Altmann, Simone</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ellinger%2C+Dorothea%22">Ellinger, Dorothea</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Eschen-Lippold%2C+Lennart%22">Eschen-Lippold, Lennart</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Miersch%2C+Otto%22">Miersch, Otto</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Scheel%2C+Dierk%22">Scheel, Dierk</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Rosahl%2C+Sabine%22">Rosahl, Sabine</searchLink><relatesTo>1</relatesTo><i> srosahl@ipb-halle.de</i>
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  Data: <searchLink fieldCode="JN" term="%22Plant+Journal%22">Plant Journal</searchLink>. Jan2009, Vol. 57 Issue 2, p230-242. 13p. 2 Diagrams, 5 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22RNA%22">RNA</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleic+acids%22">Nucleic acids</searchLink><br /><searchLink fieldCode="DE" term="%22Heredity%22">Heredity</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+death%22">Cell death</searchLink><br /><searchLink fieldCode="DE" term="%22Cells%22">Cells</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Reactive+oxygen+species%22">Reactive oxygen species</searchLink><br /><searchLink fieldCode="DE" term="%22Photosynthetic+oxygen+evolution%22">Photosynthetic oxygen evolution</searchLink><br /><searchLink fieldCode="DE" term="%22Disinfection+%26+disinfectants%22">Disinfection & disinfectants</searchLink><br /><searchLink fieldCode="DE" term="%22Salicylic+acid%22">Salicylic acid</searchLink><br /><searchLink fieldCode="DE" term="%22Transgenic+organisms%22">Transgenic organisms</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: To elucidate the molecular mechanisms underlying pathogen-associated molecular pattern (PAMP)-induced defense responses in potato ( Solanum tuberosum), the role of the signaling compounds salicylic acid (SA) and jasmonic acid (JA) was analyzed. Pep-13, a PAMP from Phytophthora, induces the accumulation of SA, JA and hydrogen peroxide, as well as the activation of defense genes and hypersensitive-like cell death. We have previously shown that SA is required for Pep-13-induced defense responses. To assess the importance of JA, RNA interference constructs targeted at the JA biosynthetic genes, allene oxide cyclase and 12-oxophytodienoic acid reductase, were expressed in transgenic potato plants. In addition, expression of the F-box protein COI1 was reduced by RNA interference. Plants expressing the RNA interference constructs failed to accumulate the respective transcripts in response to wounding or Pep-13 treatment, neither did they contain significant amounts of JA after elicitation. In response to infiltration of Pep-13, the transgenic plants exhibited a highly reduced accumulation of reactive oxygen species as well as reduced hypersensitive cell death. The ability of the JA-deficient plants to accumulate SA suggests that SA accumulation is independent or upstream of JA accumulation. These data show that PAMP responses in potato require both SA and JA and that, in contrast to Arabidopsis, these compounds act in the same signal transduction pathway. Despite their inability to fully respond to PAMP treatment, the transgenic RNA interference plants are not altered in their basal defense against Phytophthora infestans. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
  BibEntity:
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      – Type: doi
        Value: 10.1111/j.1365-313X.2008.03688.x
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      – Code: eng
        Text: English
    PhysicalDescription:
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        PageCount: 13
        StartPage: 230
    Subjects:
      – SubjectFull: RNA
        Type: general
      – SubjectFull: Nucleic acids
        Type: general
      – SubjectFull: Heredity
        Type: general
      – SubjectFull: Cell death
        Type: general
      – SubjectFull: Cells
        Type: general
      – SubjectFull: Genes
        Type: general
      – SubjectFull: Reactive oxygen species
        Type: general
      – SubjectFull: Photosynthetic oxygen evolution
        Type: general
      – SubjectFull: Disinfection & disinfectants
        Type: general
      – SubjectFull: Salicylic acid
        Type: general
      – SubjectFull: Transgenic organisms
        Type: general
    Titles:
      – TitleFull: PAMP-induced defense responses in potato require both salicylic acid and jasmonic acid.
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            NameFull: Halim, Vincentius A.
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            NameFull: Altmann, Simone
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            NameFull: Ellinger, Dorothea
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            NameFull: Miersch, Otto
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              M: 01
              Text: Jan2009
              Type: published
              Y: 2009
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