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DNA detection on ultrahigh-density optical fiber-based nanoarrays

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Title: DNA detection on ultrahigh-density optical fiber-based nanoarrays
Authors: Tam, Jenny M., Song, Linan, Walt, David R. david.walt@tufts.edu
Source: Biosensors & Bioelectronics. Apr2009, Vol. 24 Issue 8, p2488-2493. 6p.
Subjects: DNA microarrays, Optical fiber detectors, Biosensors, Bacillus thuringiensis, Vaccinia, Interleukin-2, Diagnosis of HIV infections, Bioelectronics
Abstract: Abstract: Nanoarrays for DNA detection were fabricated on etched nanofiber bundles based on recently developed techniques for microscale arrays. Two different-sized nanoarrays were created: one with 700nm feature sizes and a 1μm center-to-center pitch (∼1×106 array elements/mm2) and one with 300nm feature sizes and a 500nm center-to-center pitch (4.6×106 array elements/mm2). A random, multiplexed array composed of oligonucleotide-functionalized nanospheres was constructed and used for parallel detection and analysis of fluorescently labeled DNA targets. We have used these arrays to detect a variety of target sequences including Bacillus thuringiensis kurstaki and vaccina virus sequences, two potential biowarfare agents, as well as interleukin-2 sequences, an immune system modulator that has been used for the diagnosis of HIV. [Copyright &y& Elsevier]
Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubType: Academic Journal
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  Data: DNA detection on ultrahigh-density optical fiber-based nanoarrays
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  Data: <searchLink fieldCode="AR" term="%22Tam%2C+Jenny+M%2E%22">Tam, Jenny M.</searchLink><br /><searchLink fieldCode="AR" term="%22Song%2C+Linan%22">Song, Linan</searchLink><br /><searchLink fieldCode="AR" term="%22Walt%2C+David+R%2E%22">Walt, David R.</searchLink><i> david.walt@tufts.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Biosensors+%26+Bioelectronics%22">Biosensors & Bioelectronics</searchLink>. Apr2009, Vol. 24 Issue 8, p2488-2493. 6p.
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  Data: <searchLink fieldCode="DE" term="%22DNA+microarrays%22">DNA microarrays</searchLink><br /><searchLink fieldCode="DE" term="%22Optical+fiber+detectors%22">Optical fiber detectors</searchLink><br /><searchLink fieldCode="DE" term="%22Biosensors%22">Biosensors</searchLink><br /><searchLink fieldCode="DE" term="%22Bacillus+thuringiensis%22">Bacillus thuringiensis</searchLink><br /><searchLink fieldCode="DE" term="%22Vaccinia%22">Vaccinia</searchLink><br /><searchLink fieldCode="DE" term="%22Interleukin-2%22">Interleukin-2</searchLink><br /><searchLink fieldCode="DE" term="%22Diagnosis+of+HIV+infections%22">Diagnosis of HIV infections</searchLink><br /><searchLink fieldCode="DE" term="%22Bioelectronics%22">Bioelectronics</searchLink>
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  Data: Abstract: Nanoarrays for DNA detection were fabricated on etched nanofiber bundles based on recently developed techniques for microscale arrays. Two different-sized nanoarrays were created: one with 700nm feature sizes and a 1μm center-to-center pitch (∼1×106 array elements/mm2) and one with 300nm feature sizes and a 500nm center-to-center pitch (4.6×106 array elements/mm2). A random, multiplexed array composed of oligonucleotide-functionalized nanospheres was constructed and used for parallel detection and analysis of fluorescently labeled DNA targets. We have used these arrays to detect a variety of target sequences including Bacillus thuringiensis kurstaki and vaccina virus sequences, two potential biowarfare agents, as well as interleukin-2 sequences, an immune system modulator that has been used for the diagnosis of HIV. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
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  Data: <i>Copyright of Biosensors & Bioelectronics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.bios.2008.12.034
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      – Code: eng
        Text: English
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      – SubjectFull: DNA microarrays
        Type: general
      – SubjectFull: Optical fiber detectors
        Type: general
      – SubjectFull: Biosensors
        Type: general
      – SubjectFull: Bacillus thuringiensis
        Type: general
      – SubjectFull: Vaccinia
        Type: general
      – SubjectFull: Interleukin-2
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      – SubjectFull: Diagnosis of HIV infections
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      – SubjectFull: Bioelectronics
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      – TitleFull: DNA detection on ultrahigh-density optical fiber-based nanoarrays
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            NameFull: Tam, Jenny M.
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            NameFull: Song, Linan
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            NameFull: Walt, David R.
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              Text: Apr2009
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