DNA detection on ultrahigh-density optical fiber-based nanoarrays
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| Title: | DNA detection on ultrahigh-density optical fiber-based nanoarrays |
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| Authors: | Tam, Jenny M., Song, Linan, Walt, David R. david.walt@tufts.edu |
| Source: | Biosensors & Bioelectronics. Apr2009, Vol. 24 Issue 8, p2488-2493. 6p. |
| Subjects: | DNA microarrays, Optical fiber detectors, Biosensors, Bacillus thuringiensis, Vaccinia, Interleukin-2, Diagnosis of HIV infections, Bioelectronics |
| Abstract: | Abstract: Nanoarrays for DNA detection were fabricated on etched nanofiber bundles based on recently developed techniques for microscale arrays. Two different-sized nanoarrays were created: one with 700nm feature sizes and a 1μm center-to-center pitch (∼1×106 array elements/mm2) and one with 300nm feature sizes and a 500nm center-to-center pitch (4.6×106 array elements/mm2). A random, multiplexed array composed of oligonucleotide-functionalized nanospheres was constructed and used for parallel detection and analysis of fluorescently labeled DNA targets. We have used these arrays to detect a variety of target sequences including Bacillus thuringiensis kurstaki and vaccina virus sequences, two potential biowarfare agents, as well as interleukin-2 sequences, an immune system modulator that has been used for the diagnosis of HIV. [Copyright &y& Elsevier] |
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| Database: | Engineering Source |
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