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Ultrastructural changes in the retinopathy, globe enlarged (rge) chick cornea

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Title: Ultrastructural changes in the retinopathy, globe enlarged (rge) chick cornea
Authors: Boote, Craig1 bootec@cf.ac.uk, Hayes, Sally1, Young, Robert D.1, Kamma-Lorger, Christina S.1, Hocking, Paul M.2, Elsheikh, Ahmed3, Inglehearn, Chris F.4, Ali, Manir4, Meek, Keith M.1
Source: Journal of Structural Biology. May2009, Vol. 166 Issue 2, p195-204. 10p.
Subjects: Cornea, Ultrastructure (Biology), Retinal diseases, Collagen, Proteoglycans, Genetic mutation, Chickens as laboratory animals, X-ray scattering
Abstract: Abstract: In the cornea, the precise organisation of fibrillar collagen and associated proteoglycans comprising the stromal extracellular matrix plays a major role in governing tissue form and function. Recently, abnormal collagen alignment was noted in the misshapen corneas of mature chickens affected by the retinopathy, globe enlarged (rge) mutation. Here we further characterize corneal ultrastructural changes as the rge eye develops post-hatch. Wide-angle X-ray scattering disclosed alteration to dominant collagen lamellae directions in the rge chick cornea, compared to age-matched controls. These changes accompanied eye globe enlargement and corneal flattening in affected birds, manifesting as a progressive loss of circumferential collagen alignment in the peripheral cornea and limbus in birds older than 1month. Collagen intermolecular separation was unchanged in rge. However, small-angle X-ray scattering results suggest collagen fibril separation and diameter increase more rapidly towards the corneal periphery in rge at 3months post-hatch compared to controls, although central collagen fibril diameter was unchanged. By transmission electron microscopy utilising cuprolinic blue stain, the morphology and distribution of stromal proteoglycans were unaltered in rge corneas otherwise demonstrating abnormal collagen fibril organisation. From a numerical simulation of tissue mechanics, progressive remodelling of stromal collagen in rge during globe enlargement post-hatch appears to be related to the corneal morphometric changes presented by the disease. [Copyright &y& Elsevier]
Copyright of Journal of Structural Biology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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PubType: Academic Journal
PubTypeId: academicJournal
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  Data: Ultrastructural changes in the retinopathy, globe enlarged (rge) chick cornea
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  Data: <searchLink fieldCode="AR" term="%22Boote%2C+Craig%22">Boote, Craig</searchLink><relatesTo>1</relatesTo><i> bootec@cf.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Hayes%2C+Sally%22">Hayes, Sally</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Young%2C+Robert+D%2E%22">Young, Robert D.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Kamma-Lorger%2C+Christina+S%2E%22">Kamma-Lorger, Christina S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hocking%2C+Paul+M%2E%22">Hocking, Paul M.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Elsheikh%2C+Ahmed%22">Elsheikh, Ahmed</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Inglehearn%2C+Chris+F%2E%22">Inglehearn, Chris F.</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Ali%2C+Manir%22">Ali, Manir</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Meek%2C+Keith+M%2E%22">Meek, Keith M.</searchLink><relatesTo>1</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Structural+Biology%22">Journal of Structural Biology</searchLink>. May2009, Vol. 166 Issue 2, p195-204. 10p.
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  Data: <searchLink fieldCode="DE" term="%22Cornea%22">Cornea</searchLink><br /><searchLink fieldCode="DE" term="%22Ultrastructure+%28Biology%29%22">Ultrastructure (Biology)</searchLink><br /><searchLink fieldCode="DE" term="%22Retinal+diseases%22">Retinal diseases</searchLink><br /><searchLink fieldCode="DE" term="%22Collagen%22">Collagen</searchLink><br /><searchLink fieldCode="DE" term="%22Proteoglycans%22">Proteoglycans</searchLink><br /><searchLink fieldCode="DE" term="%22Genetic+mutation%22">Genetic mutation</searchLink><br /><searchLink fieldCode="DE" term="%22Chickens+as+laboratory+animals%22">Chickens as laboratory animals</searchLink><br /><searchLink fieldCode="DE" term="%22X-ray+scattering%22">X-ray scattering</searchLink>
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  Data: Abstract: In the cornea, the precise organisation of fibrillar collagen and associated proteoglycans comprising the stromal extracellular matrix plays a major role in governing tissue form and function. Recently, abnormal collagen alignment was noted in the misshapen corneas of mature chickens affected by the retinopathy, globe enlarged (rge) mutation. Here we further characterize corneal ultrastructural changes as the rge eye develops post-hatch. Wide-angle X-ray scattering disclosed alteration to dominant collagen lamellae directions in the rge chick cornea, compared to age-matched controls. These changes accompanied eye globe enlargement and corneal flattening in affected birds, manifesting as a progressive loss of circumferential collagen alignment in the peripheral cornea and limbus in birds older than 1month. Collagen intermolecular separation was unchanged in rge. However, small-angle X-ray scattering results suggest collagen fibril separation and diameter increase more rapidly towards the corneal periphery in rge at 3months post-hatch compared to controls, although central collagen fibril diameter was unchanged. By transmission electron microscopy utilising cuprolinic blue stain, the morphology and distribution of stromal proteoglycans were unaltered in rge corneas otherwise demonstrating abnormal collagen fibril organisation. From a numerical simulation of tissue mechanics, progressive remodelling of stromal collagen in rge during globe enlargement post-hatch appears to be related to the corneal morphometric changes presented by the disease. [Copyright &y& Elsevier]
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  Data: <i>Copyright of Journal of Structural Biology is the property of Academic Press Inc. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.jsb.2009.01.009
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        Text: English
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        PageCount: 10
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      – SubjectFull: Cornea
        Type: general
      – SubjectFull: Ultrastructure (Biology)
        Type: general
      – SubjectFull: Retinal diseases
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      – SubjectFull: Collagen
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      – SubjectFull: Proteoglycans
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      – SubjectFull: Genetic mutation
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      – SubjectFull: Chickens as laboratory animals
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      – SubjectFull: X-ray scattering
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              Text: May2009
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