Microbial production of 3-hydroxydodecanoic acid by pha operon and fadBA knockout mutant of Pseudomonas putida KT2442 harboring tesB gene.
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| Title: | Microbial production of 3-hydroxydodecanoic acid by pha operon and fadBA knockout mutant of Pseudomonas putida KT2442 harboring tesB gene. |
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| Authors: | Chung, Ahleum1, Liu, Qian2, Ouyang, Shao-Ping1, Wu, Qiong1 wuqiong@mail.tsinghua.edu.cn, Chen, Guo-Qiang chengq@mail.tsinghua.edu.cn |
| Source: | Applied Microbiology & Biotechnology. Jun2009, Vol. 83 Issue 3, p513-519. 7p. 1 Diagram, 4 Charts, 1 Graph. |
| Subjects: | Poly-beta-hydroxyalkanoates, Operons, Pseudomonas, Genes, Fermentation, Oxidation, Dehydrogenases, Monomers |
| Abstract: | To produce extracellular chiral 3-hydroxyacyl acids (3HA) by fermentation, a novel pathway was constructed by expressing tesB gene encoding thioesterase II into Pseudomonas putida KTOY01, which was a polyhydroxyalkanoate (PHA) synthesis operon knockout mutant. 3HA mixtures of 0.35 g/l consisting of 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, and 3-hydroxydodecanoate (3HDD) were produced in shake-flask study using dodecanoate as a sole carbon source. Additional knockout of fadB and fadA genes encoding 3-ketoacyl-CoA thiolase and 3-hydroxyacyl-CoA dehydrogenase in P. putida KTOY01 led to the weakening of the β-oxidation pathway. The fadBA and PHA synthesis operon knockout mutant P. putida KTOY07 expressing tesB gene produced 2.44 g/l 3HA, significantly more than that of the β-oxidation intact mutant. The 3HA mixture contained 90 mol% 3HDD as a dominant component. A fed-batch fermentation process carried out in a 6-l automatic fermentor produced 7.27 g/l extracellular 3HA containing 96 mol% fraction of 3HDD after 28 h of growth. For the first time, it became possible to produce 3HDD-dominant 3HA monomers. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 40076840 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Microbial production of 3-hydroxydodecanoic acid by pha operon and fadBA knockout mutant of Pseudomonas putida KT2442 harboring tesB gene. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Chung%2C+Ahleum%22">Chung, Ahleum</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Qian%22">Liu, Qian</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Ouyang%2C+Shao-Ping%22">Ouyang, Shao-Ping</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Qiong%22">Wu, Qiong</searchLink><relatesTo>1</relatesTo><i> wuqiong@mail.tsinghua.edu.cn</i><br /><searchLink fieldCode="AR" term="%22Chen%2C+Guo-Qiang%22">Chen, Guo-Qiang</searchLink><i> chengq@mail.tsinghua.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Jun2009, Vol. 83 Issue 3, p513-519. 7p. 1 Diagram, 4 Charts, 1 Graph. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Poly-beta-hydroxyalkanoates%22">Poly-beta-hydroxyalkanoates</searchLink><br /><searchLink fieldCode="DE" term="%22Operons%22">Operons</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudomonas%22">Pseudomonas</searchLink><br /><searchLink fieldCode="DE" term="%22Genes%22">Genes</searchLink><br /><searchLink fieldCode="DE" term="%22Fermentation%22">Fermentation</searchLink><br /><searchLink fieldCode="DE" term="%22Oxidation%22">Oxidation</searchLink><br /><searchLink fieldCode="DE" term="%22Dehydrogenases%22">Dehydrogenases</searchLink><br /><searchLink fieldCode="DE" term="%22Monomers%22">Monomers</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: To produce extracellular chiral 3-hydroxyacyl acids (3HA) by fermentation, a novel pathway was constructed by expressing tesB gene encoding thioesterase II into Pseudomonas putida KTOY01, which was a polyhydroxyalkanoate (PHA) synthesis operon knockout mutant. 3HA mixtures of 0.35 g/l consisting of 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, and 3-hydroxydodecanoate (3HDD) were produced in shake-flask study using dodecanoate as a sole carbon source. Additional knockout of fadB and fadA genes encoding 3-ketoacyl-CoA thiolase and 3-hydroxyacyl-CoA dehydrogenase in P. putida KTOY01 led to the weakening of the β-oxidation pathway. The fadBA and PHA synthesis operon knockout mutant P. putida KTOY07 expressing tesB gene produced 2.44 g/l 3HA, significantly more than that of the β-oxidation intact mutant. The 3HA mixture contained 90 mol% 3HDD as a dominant component. A fed-batch fermentation process carried out in a 6-l automatic fermentor produced 7.27 g/l extracellular 3HA containing 96 mol% fraction of 3HDD after 28 h of growth. For the first time, it became possible to produce 3HDD-dominant 3HA monomers. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-009-1919-6 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 513 Subjects: – SubjectFull: Poly-beta-hydroxyalkanoates Type: general – SubjectFull: Operons Type: general – SubjectFull: Pseudomonas Type: general – SubjectFull: Genes Type: general – SubjectFull: Fermentation Type: general – SubjectFull: Oxidation Type: general – SubjectFull: Dehydrogenases Type: general – SubjectFull: Monomers Type: general Titles: – TitleFull: Microbial production of 3-hydroxydodecanoic acid by pha operon and fadBA knockout mutant of Pseudomonas putida KT2442 harboring tesB gene. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Chung, Ahleum – PersonEntity: Name: NameFull: Liu, Qian – PersonEntity: Name: NameFull: Ouyang, Shao-Ping – PersonEntity: Name: NameFull: Wu, Qiong – PersonEntity: Name: NameFull: Chen, Guo-Qiang IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 06 Text: Jun2009 Type: published Y: 2009 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 83 – Type: issue Value: 3 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
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