View in EDS

Comparative day/night metatranscriptomic analysis of microbial communities in the North Pacific subtropical gyre.

Saved in:
Bibliographic Details
Title: Comparative day/night metatranscriptomic analysis of microbial communities in the North Pacific subtropical gyre.
Authors: Poretsky, Rachel S.1, Hewson, Ian2, Sun, Shulei1, Allen, Andrew E.3, Zehr, Jonathan P.2, Moran, Mary Ann1 mmoran@uga.edu
Source: Environmental Microbiology. Jun2009, Vol. 11 Issue 6, p1358-1375. 18p. 3 Diagrams, 4 Charts, 4 Graphs.
Subjects: Gene expression, Messenger RNA, Cyanobacteria, Photosynthesis, Nutrient cycles, Marine microbiology, Biogeochemistry, Chlorophyll, Phosphorylation, Biosynthesis
Abstract: Metatranscriptomic analyses of microbial assemblages (< 5 μm) from surface water at the Hawaiian Ocean Time-Series (HOT) revealed community-wide metabolic activities and day/night patterns of differential gene expression. Pyrosequencing produced 75 558 putative mRNA reads from a day transcriptome and 75 946 from a night transcriptome. Taxonomic binning of annotated mRNAs indicated that Cyanobacteria contributed a greater percentage of the transcripts (54% of annotated sequences) than expected based on abundance (35% of cell counts and 21% 16S rRNA of libraries), and may represent the most actively transcribing cells in this surface ocean community in both the day and night. Major heterotrophic taxa contributing to the community transcriptome included α -Proteobacteria (19% of annotated sequences, most of which were SAR11-related) and γ -Proteobacteria (4%). The composition of transcript pools was consistent with models of prokaryotic gene expression, including operon-based transcription patterns and an abundance of genes predicted to be highly expressed. Metabolic activities that are shared by many microbial taxa (e.g. glycolysis, citric acid cycle, amino acid biosynthesis and transcription and translation machinery) were well represented among the community transcripts. There was an overabundance of transcripts for photosynthesis, C1 metabolism and oxidative phosphorylation in the day compared with night, and evidence that energy acquisition is coordinated with solar radiation levels for both autotrophic and heterotrophic microbes. In contrast, housekeeping activities such as amino acid biosynthesis, membrane synthesis and repair, and vitamin biosynthesis were overrepresented in the night transcriptome. Direct sequencing of these environmental transcripts has provided detailed information on metabolic and biogeochemical responses of a microbial community to solar forcing. [ABSTRACT FROM AUTHOR]
Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 40642426
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: Comparative day/night metatranscriptomic analysis of microbial communities in the North Pacific subtropical gyre.
– Name: Author
  Label: Authors
  Group: Au
  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Poretsky%2C+Rachel+S%2E%22&quot;&gt;Poretsky, Rachel S.&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Hewson%2C+Ian%22&quot;&gt;Hewson, Ian&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Sun%2C+Shulei%22&quot;&gt;Sun, Shulei&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Allen%2C+Andrew+E%2E%22&quot;&gt;Allen, Andrew E.&lt;/searchLink&gt;&lt;relatesTo&gt;3&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zehr%2C+Jonathan+P%2E%22&quot;&gt;Zehr, Jonathan P.&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Moran%2C+Mary+Ann%22&quot;&gt;Moran, Mary Ann&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;i&gt; mmoran@uga.edu&lt;/i&gt;
– Name: TitleSource
  Label: Source
  Group: Src
  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Environmental+Microbiology%22&quot;&gt;Environmental Microbiology&lt;/searchLink&gt;. Jun2009, Vol. 11 Issue 6, p1358-1375. 18p. 3 Diagrams, 4 Charts, 4 Graphs.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Gene+expression%22&quot;&gt;Gene expression&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Messenger+RNA%22&quot;&gt;Messenger RNA&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Cyanobacteria%22&quot;&gt;Cyanobacteria&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Photosynthesis%22&quot;&gt;Photosynthesis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Nutrient+cycles%22&quot;&gt;Nutrient cycles&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Marine+microbiology%22&quot;&gt;Marine microbiology&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Biogeochemistry%22&quot;&gt;Biogeochemistry&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Chlorophyll%22&quot;&gt;Chlorophyll&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Phosphorylation%22&quot;&gt;Phosphorylation&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Biosynthesis%22&quot;&gt;Biosynthesis&lt;/searchLink&gt;
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Metatranscriptomic analyses of microbial assemblages (&lt; 5 μm) from surface water at the Hawaiian Ocean Time-Series (HOT) revealed community-wide metabolic activities and day/night patterns of differential gene expression. Pyrosequencing produced 75 558 putative mRNA reads from a day transcriptome and 75 946 from a night transcriptome. Taxonomic binning of annotated mRNAs indicated that Cyanobacteria contributed a greater percentage of the transcripts (54% of annotated sequences) than expected based on abundance (35% of cell counts and 21% 16S rRNA of libraries), and may represent the most actively transcribing cells in this surface ocean community in both the day and night. Major heterotrophic taxa contributing to the community transcriptome included α -Proteobacteria (19% of annotated sequences, most of which were SAR11-related) and γ -Proteobacteria (4%). The composition of transcript pools was consistent with models of prokaryotic gene expression, including operon-based transcription patterns and an abundance of genes predicted to be highly expressed. Metabolic activities that are shared by many microbial taxa (e.g. glycolysis, citric acid cycle, amino acid biosynthesis and transcription and translation machinery) were well represented among the community transcripts. There was an overabundance of transcripts for photosynthesis, C1 metabolism and oxidative phosphorylation in the day compared with night, and evidence that energy acquisition is coordinated with solar radiation levels for both autotrophic and heterotrophic microbes. In contrast, housekeeping activities such as amino acid biosynthesis, membrane synthesis and repair, and vitamin biosynthesis were overrepresented in the night transcriptome. Direct sequencing of these environmental transcripts has provided detailed information on metabolic and biogeochemical responses of a microbial community to solar forcing. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: &lt;i&gt;Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=40642426
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1111/j.1462-2920.2008.01863.x
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 18
        StartPage: 1358
    Subjects:
      – SubjectFull: Gene expression
        Type: general
      – SubjectFull: Messenger RNA
        Type: general
      – SubjectFull: Cyanobacteria
        Type: general
      – SubjectFull: Photosynthesis
        Type: general
      – SubjectFull: Nutrient cycles
        Type: general
      – SubjectFull: Marine microbiology
        Type: general
      – SubjectFull: Biogeochemistry
        Type: general
      – SubjectFull: Chlorophyll
        Type: general
      – SubjectFull: Phosphorylation
        Type: general
      – SubjectFull: Biosynthesis
        Type: general
    Titles:
      – TitleFull: Comparative day/night metatranscriptomic analysis of microbial communities in the North Pacific subtropical gyre.
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Poretsky, Rachel S.
      – PersonEntity:
          Name:
            NameFull: Hewson, Ian
      – PersonEntity:
          Name:
            NameFull: Sun, Shulei
      – PersonEntity:
          Name:
            NameFull: Allen, Andrew E.
      – PersonEntity:
          Name:
            NameFull: Zehr, Jonathan P.
      – PersonEntity:
          Name:
            NameFull: Moran, Mary Ann
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 06
              Text: Jun2009
              Type: published
              Y: 2009
          Identifiers:
            – Type: issn-print
              Value: 14622912
          Numbering:
            – Type: volume
              Value: 11
            – Type: issue
              Value: 6
          Titles:
            – TitleFull: Environmental Microbiology
              Type: main
ResultId 1