Coupled human erythrocyte velocity field and aggregation measurements at physiological haematocrit levels
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| Title: | Coupled human erythrocyte velocity field and aggregation measurements at physiological haematocrit levels |
|---|---|
| Authors: | Dusting, Jonathan Jonathan.dusting@kcl.ac.uk, Kaliviotis, Efstathios1, Balabani, Stavroula1, Yianneskis, Michael1 |
| Source: | Journal of Biomechanics. Jul2009, Vol. 42 Issue 10, p1438-1443. 6p. |
| Subjects: | Erythrocytes, Cell aggregation, Hematocrit, Particle image velocimetry, Microscopy, Image processing, Blood flow |
| Abstract: | Abstract: Simultaneous measurement of erythrocyte (RBC) velocity fields and aggregation properties has been successfully performed using an optical shearing microscope and Particle Image Velocimetry (PIV). Blood at haematocrit was sheared at rates of and imaged using a high speed camera. The images were then processed to yield aggregation indices and flow velocities. Negligible levels of aggregation were observed for , while high levels of aggregation and network formation occurred for . The results illustrate that the velocity measurements are dependent on the extent of RBC aggregation. High levels of network formation cause the velocities at to deviate markedly from the expected solid body rotation profile. The effect of aggregation level on the PIV accuracy was assessed by monitoring the two-dimensional (2D) correlation coefficients. Lower levels of aggregation result in poorer image correlation, from which it can be inferred that PIV accuracy is reduced. Moreover, aggregation is time-dependent, and consequently PIV accuracy may decrease during recording as the cells break up. It is therefore recommended that aggregation and its effects are taken into account in future when undertaking blood flow studies using PIV. The simplicity of the technique, which requires no lasers, filters, or special pretreatments, demonstrates the potential wide-spread applicability of the data acquisition system for accurate blood flow PIV and aggregation measurement. [Copyright &y& Elsevier] |
| Copyright of Journal of Biomechanics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 43176568 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Coupled human erythrocyte velocity field and aggregation measurements at physiological haematocrit levels – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Dusting%2C+Jonathan%22">Dusting, Jonathan</searchLink><i> Jonathan.dusting@kcl.ac.uk</i><br /><searchLink fieldCode="AR" term="%22Kaliviotis%2C+Efstathios%22">Kaliviotis, Efstathios</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Balabani%2C+Stavroula%22">Balabani, Stavroula</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yianneskis%2C+Michael%22">Yianneskis, Michael</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Journal+of+Biomechanics%22">Journal of Biomechanics</searchLink>. Jul2009, Vol. 42 Issue 10, p1438-1443. 6p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Erythrocytes%22">Erythrocytes</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+aggregation%22">Cell aggregation</searchLink><br /><searchLink fieldCode="DE" term="%22Hematocrit%22">Hematocrit</searchLink><br /><searchLink fieldCode="DE" term="%22Particle+image+velocimetry%22">Particle image velocimetry</searchLink><br /><searchLink fieldCode="DE" term="%22Microscopy%22">Microscopy</searchLink><br /><searchLink fieldCode="DE" term="%22Image+processing%22">Image processing</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+flow%22">Blood flow</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Abstract: Simultaneous measurement of erythrocyte (RBC) velocity fields and aggregation properties has been successfully performed using an optical shearing microscope and Particle Image Velocimetry (PIV). Blood at haematocrit was sheared at rates of and imaged using a high speed camera. The images were then processed to yield aggregation indices and flow velocities. Negligible levels of aggregation were observed for , while high levels of aggregation and network formation occurred for . The results illustrate that the velocity measurements are dependent on the extent of RBC aggregation. High levels of network formation cause the velocities at to deviate markedly from the expected solid body rotation profile. The effect of aggregation level on the PIV accuracy was assessed by monitoring the two-dimensional (2D) correlation coefficients. Lower levels of aggregation result in poorer image correlation, from which it can be inferred that PIV accuracy is reduced. Moreover, aggregation is time-dependent, and consequently PIV accuracy may decrease during recording as the cells break up. It is therefore recommended that aggregation and its effects are taken into account in future when undertaking blood flow studies using PIV. The simplicity of the technique, which requires no lasers, filters, or special pretreatments, demonstrates the potential wide-spread applicability of the data acquisition system for accurate blood flow PIV and aggregation measurement. [Copyright &y& Elsevier] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Journal of Biomechanics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1016/j.jbiomech.2009.04.004 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 6 StartPage: 1438 Subjects: – SubjectFull: Erythrocytes Type: general – SubjectFull: Cell aggregation Type: general – SubjectFull: Hematocrit Type: general – SubjectFull: Particle image velocimetry Type: general – SubjectFull: Microscopy Type: general – SubjectFull: Image processing Type: general – SubjectFull: Blood flow Type: general Titles: – TitleFull: Coupled human erythrocyte velocity field and aggregation measurements at physiological haematocrit levels Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Dusting, Jonathan – PersonEntity: Name: NameFull: Kaliviotis, Efstathios – PersonEntity: Name: NameFull: Balabani, Stavroula – PersonEntity: Name: NameFull: Yianneskis, Michael IsPartOfRelationships: – BibEntity: Dates: – D: 22 M: 07 Text: Jul2009 Type: published Y: 2009 Identifiers: – Type: issn-print Value: 00219290 Numbering: – Type: volume Value: 42 – Type: issue Value: 10 Titles: – TitleFull: Journal of Biomechanics Type: main |
| ResultId | 1 |
