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In vitro and in vivo performance of biocompatible negatively-charged salbutamol-loaded nanoparticles

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Title: In vitro and in vivo performance of biocompatible negatively-charged salbutamol-loaded nanoparticles
Authors: Rytting, Erik1, Bur, Michael2, Cartier, Regis3, Bouyssou, Thierry4, Wang, Xiaoying1, Krüger, Michael3, Lehr, Claus-Michael2, Kissel, Thomas1 kissel@staff.uni-marburg.de
Source: Journal of Controlled Release. Jan2010, Vol. 141 Issue 1, p101-107. 7p.
Subjects: Biomedical materials, Nanoparticles, Biodegradation, Guinea pigs as laboratory animals, Nanomedicine, Drug delivery systems, Biocompatibility
Abstract: Abstract: The development and performance of a novel nanoparticle-based formulation for pulmonary delivery has been characterized chronologically through the particle preparation process, in vitro testing of drug release, biocompatibility, degradation, drug transport in cell culture, and in vivo bronchoprotection studies in anaesthetised guinea pigs. This study demonstrates excellent agreement of the in vitro and in vivo experiments undertaken to prove the feasibility of the design, thereby serving as an example highlighting the importance of in vitro test methods that predict in vivo performance. Nanoparticles were prepared from the newly designed negatively-charged polymer poly(vinyl sulfonate-co-vinyl alcohol)-g-poly(d,l-lactic-co-glycolic acid) loaded with salbutamol free base. Average particle sizes of blank and drug-loaded nanoparticles prepared at the various stages of the investigations were between 91 and 204nm; average zeta potential values were between −50.1 and −25.6mV. Blank nanoparticles showed no significant toxicity, and no inflammatory activity was detected in Calu-3 cells. Sustained release of salbutamol from the nanoparticles was observed for 2.5h in vitro, and a prolonged effect was observed for 120min in vivo. These results demonstrate good agreement between in vitro and in vivo tests and also present a promising foundation for future advancement in nanomedicine strategies for pulmonary drug delivery. [Copyright &y& Elsevier]
Copyright of Journal of Controlled Release is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 45638509
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: In vitro and in vivo performance of biocompatible negatively-charged salbutamol-loaded nanoparticles
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  Data: <searchLink fieldCode="AR" term="%22Rytting%2C+Erik%22">Rytting, Erik</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Bur%2C+Michael%22">Bur, Michael</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Cartier%2C+Regis%22">Cartier, Regis</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Bouyssou%2C+Thierry%22">Bouyssou, Thierry</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Xiaoying%22">Wang, Xiaoying</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Krüger%2C+Michael%22">Krüger, Michael</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Lehr%2C+Claus-Michael%22">Lehr, Claus-Michael</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kissel%2C+Thomas%22">Kissel, Thomas</searchLink><relatesTo>1</relatesTo><i> kissel@staff.uni-marburg.de</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Controlled+Release%22">Journal of Controlled Release</searchLink>. Jan2010, Vol. 141 Issue 1, p101-107. 7p.
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  Data: <searchLink fieldCode="DE" term="%22Biomedical+materials%22">Biomedical materials</searchLink><br /><searchLink fieldCode="DE" term="%22Nanoparticles%22">Nanoparticles</searchLink><br /><searchLink fieldCode="DE" term="%22Biodegradation%22">Biodegradation</searchLink><br /><searchLink fieldCode="DE" term="%22Guinea+pigs+as+laboratory+animals%22">Guinea pigs as laboratory animals</searchLink><br /><searchLink fieldCode="DE" term="%22Nanomedicine%22">Nanomedicine</searchLink><br /><searchLink fieldCode="DE" term="%22Drug+delivery+systems%22">Drug delivery systems</searchLink><br /><searchLink fieldCode="DE" term="%22Biocompatibility%22">Biocompatibility</searchLink>
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  Data: Abstract: The development and performance of a novel nanoparticle-based formulation for pulmonary delivery has been characterized chronologically through the particle preparation process, in vitro testing of drug release, biocompatibility, degradation, drug transport in cell culture, and in vivo bronchoprotection studies in anaesthetised guinea pigs. This study demonstrates excellent agreement of the in vitro and in vivo experiments undertaken to prove the feasibility of the design, thereby serving as an example highlighting the importance of in vitro test methods that predict in vivo performance. Nanoparticles were prepared from the newly designed negatively-charged polymer poly(vinyl sulfonate-co-vinyl alcohol)-g-poly(d,l-lactic-co-glycolic acid) loaded with salbutamol free base. Average particle sizes of blank and drug-loaded nanoparticles prepared at the various stages of the investigations were between 91 and 204nm; average zeta potential values were between −50.1 and −25.6mV. Blank nanoparticles showed no significant toxicity, and no inflammatory activity was detected in Calu-3 cells. Sustained release of salbutamol from the nanoparticles was observed for 2.5h in vitro, and a prolonged effect was observed for 120min in vivo. These results demonstrate good agreement between in vitro and in vivo tests and also present a promising foundation for future advancement in nanomedicine strategies for pulmonary drug delivery. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Controlled Release is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.jconrel.2009.08.021
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      – Code: eng
        Text: English
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        PageCount: 7
        StartPage: 101
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        Type: general
      – SubjectFull: Nanoparticles
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      – SubjectFull: Biodegradation
        Type: general
      – SubjectFull: Guinea pigs as laboratory animals
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      – SubjectFull: Nanomedicine
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      – SubjectFull: Drug delivery systems
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      – SubjectFull: Biocompatibility
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      – TitleFull: In vitro and in vivo performance of biocompatible negatively-charged salbutamol-loaded nanoparticles
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              Text: Jan2010
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              Y: 2010
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