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Further refinement of the non-invasive procedure for measuring steroid production in the male three-spined stickleback Gasterosteus aculeatus.

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Title: Further refinement of the non-invasive procedure for measuring steroid production in the male three-spined stickleback Gasterosteus aculeatus.
Authors: Sebire, M.1, Katsiadaki, I.2, Scott, A. P.2 alexander.scott@cefas.co.uk
Source: Journal of Fish Biology. Nov2009, Vol. 75 Issue 8, p2082-2094. 13p. 1 Diagram, 1 Chart, 5 Graphs.
Subjects: Threespine stickleback, Endocrinology of human reproduction, Radioimmunoassay, Stickleback behavior, Testosterone
Abstract: Measurement of steroids that are released into the water via the gills has previously been shown to be an effective way of studying the reproductive endocrinology of the male three-spined stickleback Gasterosteus aculeatus without having to kill the fish. In the present paper, a previous observation on the existence of a compound other than 11-ketotestosterone (11-KT) in water, which cross-reacted in the 11-KT radioimmunoassay was repeated. The amounts of this compound, however, were not sufficient to warrant a separation step prior to carrying out assay. The lack of association between androstenedione levels in water and those in plasma was also confirmed. For the first time, the amounts of testosterone released into the water were shown to be positively correlated with the amounts in plasma, the sampling procedure (placing the fish for 30 min in 50 ml water) had no effect on the rate of release of cortisol but caused a rapid drop in the rate of release of 11-KT (which means that the fish should not be sampled twice in short succession), physical interaction between two nesting males (which was accompanied by aggression) significantly increased the rate of release of 11-KT, androstenedione and testosterone (but not of cortisol) and the rate of release of 11-KT was at its maximum between 2 and 4 h after exposure. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Fish Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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DbLabel: Engineering Source
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PubType: Academic Journal
PubTypeId: academicJournal
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  Label: Title
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  Data: Further refinement of the non-invasive procedure for measuring steroid production in the male three-spined stickleback Gasterosteus aculeatus.
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  Data: <searchLink fieldCode="AR" term="%22Sebire%2C+M%2E%22">Sebire, M.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Katsiadaki%2C+I%2E%22">Katsiadaki, I.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Scott%2C+A%2E+P%2E%22">Scott, A. P.</searchLink><relatesTo>2</relatesTo><i> alexander.scott@cefas.co.uk</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Fish+Biology%22">Journal of Fish Biology</searchLink>. Nov2009, Vol. 75 Issue 8, p2082-2094. 13p. 1 Diagram, 1 Chart, 5 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Threespine+stickleback%22">Threespine stickleback</searchLink><br /><searchLink fieldCode="DE" term="%22Endocrinology+of+human+reproduction%22">Endocrinology of human reproduction</searchLink><br /><searchLink fieldCode="DE" term="%22Radioimmunoassay%22">Radioimmunoassay</searchLink><br /><searchLink fieldCode="DE" term="%22Stickleback+behavior%22">Stickleback behavior</searchLink><br /><searchLink fieldCode="DE" term="%22Testosterone%22">Testosterone</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Measurement of steroids that are released into the water via the gills has previously been shown to be an effective way of studying the reproductive endocrinology of the male three-spined stickleback Gasterosteus aculeatus without having to kill the fish. In the present paper, a previous observation on the existence of a compound other than 11-ketotestosterone (11-KT) in water, which cross-reacted in the 11-KT radioimmunoassay was repeated. The amounts of this compound, however, were not sufficient to warrant a separation step prior to carrying out assay. The lack of association between androstenedione levels in water and those in plasma was also confirmed. For the first time, the amounts of testosterone released into the water were shown to be positively correlated with the amounts in plasma, the sampling procedure (placing the fish for 30 min in 50 ml water) had no effect on the rate of release of cortisol but caused a rapid drop in the rate of release of 11-KT (which means that the fish should not be sampled twice in short succession), physical interaction between two nesting males (which was accompanied by aggression) significantly increased the rate of release of 11-KT, androstenedione and testosterone (but not of cortisol) and the rate of release of 11-KT was at its maximum between 2 and 4 h after exposure. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Journal of Fish Biology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1111/j.1095-8649.2009.02409.x
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 13
        StartPage: 2082
    Subjects:
      – SubjectFull: Threespine stickleback
        Type: general
      – SubjectFull: Endocrinology of human reproduction
        Type: general
      – SubjectFull: Radioimmunoassay
        Type: general
      – SubjectFull: Stickleback behavior
        Type: general
      – SubjectFull: Testosterone
        Type: general
    Titles:
      – TitleFull: Further refinement of the non-invasive procedure for measuring steroid production in the male three-spined stickleback Gasterosteus aculeatus.
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            NameFull: Sebire, M.
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            NameFull: Katsiadaki, I.
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            NameFull: Scott, A. P.
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            – D: 15
              M: 11
              Text: Nov2009
              Type: published
              Y: 2009
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              Value: 75
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              Value: 8
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            – TitleFull: Journal of Fish Biology
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