Transporter genes expressed by coastal bacterioplankton in response to dissolved organic carbon.
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| Title: | Transporter genes expressed by coastal bacterioplankton in response to dissolved organic carbon. |
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| Authors: | Poretsky, Rachel S.1, Sun, Shulei2, Mou, Xiaozhen3, Moran, Mary Ann4 mmoran@uga.edu |
| Source: | Environmental Microbiology. Mar2010, Vol. 12 Issue 3, p616-627. 12p. 1 Diagram, 2 Charts, 4 Graphs. |
| Subjects: | Plankton, Bacteria, Carbon compounds, Food chains, Marine ecology, Gene expression |
| Abstract: | Coastal ocean bacterioplankton control the flow of dissolved organic carbon (DOC) from terrestrial and oceanic sources into the marine food web, and regulate the release of inorganic carbon to atmospheric and offshore reservoirs. While the fate of the chemically complex coastal DOC reservoir has long been recognized as a critical feature of the global carbon budget, it has been problematic to identify both the compounds that serve as major conduits for carbon flux and the roles of individual bacterioplankton taxa in mediating that flux. Here we analyse random libraries of expressed genes from a coastal bacterial community to identify sequences representing DOC-transporting proteins. Predicted substrates of expressed transporter genes indicated that carboxylic acids, compatible solutes, polyamines and lipids may be key components of the biologically labile DOC pool in coastal waters, in addition to canonical bacterial substrates such as amino acids, oligopeptides and carbohydrates. Half of the expressed DOC transporter sequences in this coastal ocean appeared to originate from just eight taxa: Roseobacter, SAR11, Flavobacteriales and five orders of γ- Proteobacteria. While all major taxa expressed transporter genes for some DOC components (e.g. amino acids), there were indications of specialization within the bacterioplankton community for others (e.g. carbohydrates, carboxylic acids and polyamines). Experimental manipulations of the natural DOC pool that increased the concentration of phytoplankton- or vascular plant-derived compounds invoked a readily measured response in bacterial transporter gene expression. This highly resolved view of the potential for carbon flux into heterotrophic bacterioplankton cells identifies possible bioreactive components of the coastal DOC pool and highlights differing ecological roles in carbon turnover for the resident bacterial taxa. [ABSTRACT FROM AUTHOR] |
| Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 48303740 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Transporter genes expressed by coastal bacterioplankton in response to dissolved organic carbon. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Poretsky%2C+Rachel+S%2E%22">Poretsky, Rachel S.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Sun%2C+Shulei%22">Sun, Shulei</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Mou%2C+Xiaozhen%22">Mou, Xiaozhen</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Moran%2C+Mary+Ann%22">Moran, Mary Ann</searchLink><relatesTo>4</relatesTo><i> mmoran@uga.edu</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Environmental+Microbiology%22">Environmental Microbiology</searchLink>. Mar2010, Vol. 12 Issue 3, p616-627. 12p. 1 Diagram, 2 Charts, 4 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Plankton%22">Plankton</searchLink><br /><searchLink fieldCode="DE" term="%22Bacteria%22">Bacteria</searchLink><br /><searchLink fieldCode="DE" term="%22Carbon+compounds%22">Carbon compounds</searchLink><br /><searchLink fieldCode="DE" term="%22Food+chains%22">Food chains</searchLink><br /><searchLink fieldCode="DE" term="%22Marine+ecology%22">Marine ecology</searchLink><br /><searchLink fieldCode="DE" term="%22Gene+expression%22">Gene expression</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Coastal ocean bacterioplankton control the flow of dissolved organic carbon (DOC) from terrestrial and oceanic sources into the marine food web, and regulate the release of inorganic carbon to atmospheric and offshore reservoirs. While the fate of the chemically complex coastal DOC reservoir has long been recognized as a critical feature of the global carbon budget, it has been problematic to identify both the compounds that serve as major conduits for carbon flux and the roles of individual bacterioplankton taxa in mediating that flux. Here we analyse random libraries of expressed genes from a coastal bacterial community to identify sequences representing DOC-transporting proteins. Predicted substrates of expressed transporter genes indicated that carboxylic acids, compatible solutes, polyamines and lipids may be key components of the biologically labile DOC pool in coastal waters, in addition to canonical bacterial substrates such as amino acids, oligopeptides and carbohydrates. Half of the expressed DOC transporter sequences in this coastal ocean appeared to originate from just eight taxa: Roseobacter, SAR11, Flavobacteriales and five orders of γ- Proteobacteria. While all major taxa expressed transporter genes for some DOC components (e.g. amino acids), there were indications of specialization within the bacterioplankton community for others (e.g. carbohydrates, carboxylic acids and polyamines). Experimental manipulations of the natural DOC pool that increased the concentration of phytoplankton- or vascular plant-derived compounds invoked a readily measured response in bacterial transporter gene expression. This highly resolved view of the potential for carbon flux into heterotrophic bacterioplankton cells identifies possible bioreactive components of the coastal DOC pool and highlights differing ecological roles in carbon turnover for the resident bacterial taxa. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Environmental Microbiology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1111/j.1462-2920.2009.02102.x Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 12 StartPage: 616 Subjects: – SubjectFull: Plankton Type: general – SubjectFull: Bacteria Type: general – SubjectFull: Carbon compounds Type: general – SubjectFull: Food chains Type: general – SubjectFull: Marine ecology Type: general – SubjectFull: Gene expression Type: general Titles: – TitleFull: Transporter genes expressed by coastal bacterioplankton in response to dissolved organic carbon. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Poretsky, Rachel S. – PersonEntity: Name: NameFull: Sun, Shulei – PersonEntity: Name: NameFull: Mou, Xiaozhen – PersonEntity: Name: NameFull: Moran, Mary Ann IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2010 Type: published Y: 2010 Identifiers: – Type: issn-print Value: 14622912 Numbering: – Type: volume Value: 12 – Type: issue Value: 3 Titles: – TitleFull: Environmental Microbiology Type: main |
| ResultId | 1 |
