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The transient nature of the oligogalaturonide-induced ion fluxes of tobacco cells is not correlated with fragmentation of the oligogalacturonides.

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Title: The transient nature of the oligogalaturonide-induced ion fluxes of tobacco cells is not correlated with fragmentation of the oligogalacturonides.
Authors: Mathieu, Yves1,2, Guern, Jean1, Spiro, Mark D.2, O’Neill, Malcolm A.2, Kates, Keith2, Darvill, Alan G.2, Albersheim, Peter2
Source: Plant Journal. Nov98, Vol. 16 Issue 3, p305-311. 7p. 2 Charts, 6 Graphs.
Subjects: Galactans, Tobacco, Cytology
Abstract: SummaryThe metabolism by suspension-cultured tobacco cells of oligogalacturonides was investigated. Dodecagalacturonic acid-[3H]galactitol induces a rapid and transient alkalinization of the incubation medium resulting in part from enhanced K+ efflux from tobacco cells. However, a threefold higher concentration of dodecagalacturonic acid-[3H]galactitol is required to induce a response with the same amplitude and kinetics as that induced by the unreduced tridecagalacturonic acid. Approximately 20% of the dodecagalacturonic acid-[3H]galactitol added to suspension-cultured tobacco ionically binds to the cell walls within 1 min; maximum binding (approximately 30% of the oligogalacturonide) occurs in approximately 25 min. The unbound dodecagalacturonic acid-[3H]galactitol is rapidly (half-life, 30 min) fragmented to smaller, biologically inactive fragments by a polygalacturonase present in the growth medium. In contrast, the wall-bound dodecagalacturonic acid-[3H]galactitol is not degraded for at least 150 min. However, the kinetics, amplitude and duration of oligogalacturonide-induced ion fluxes are not correlated with the rate at which oligogalacturonides are converted to biologically inactive fragments. We propose that the transient nature of the oligogalacturonide-induced responses is likely to result from a temporary desensitization of the plant cells to the bioactive oligogalacturonides. [ABSTRACT FROM AUTHOR]
Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 5089720
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: The transient nature of the oligogalaturonide-induced ion fluxes of tobacco cells is not correlated with fragmentation of the oligogalacturonides.
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  Data: <searchLink fieldCode="AR" term="%22Mathieu%2C+Yves%22">Mathieu, Yves</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Guern%2C+Jean%22">Guern, Jean</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Spiro%2C+Mark+D%2E%22">Spiro, Mark D.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22O’Neill%2C+Malcolm+A%2E%22">O’Neill, Malcolm A.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kates%2C+Keith%22">Kates, Keith</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Darvill%2C+Alan+G%2E%22">Darvill, Alan G.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Albersheim%2C+Peter%22">Albersheim, Peter</searchLink><relatesTo>2</relatesTo>
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  Data: <searchLink fieldCode="JN" term="%22Plant+Journal%22">Plant Journal</searchLink>. Nov98, Vol. 16 Issue 3, p305-311. 7p. 2 Charts, 6 Graphs.
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  Data: <searchLink fieldCode="DE" term="%22Galactans%22">Galactans</searchLink><br /><searchLink fieldCode="DE" term="%22Tobacco%22">Tobacco</searchLink><br /><searchLink fieldCode="DE" term="%22Cytology%22">Cytology</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: SummaryThe metabolism by suspension-cultured tobacco cells of oligogalacturonides was investigated. Dodecagalacturonic acid-[3H]galactitol induces a rapid and transient alkalinization of the incubation medium resulting in part from enhanced K+ efflux from tobacco cells. However, a threefold higher concentration of dodecagalacturonic acid-[3H]galactitol is required to induce a response with the same amplitude and kinetics as that induced by the unreduced tridecagalacturonic acid. Approximately 20% of the dodecagalacturonic acid-[3H]galactitol added to suspension-cultured tobacco ionically binds to the cell walls within 1 min; maximum binding (approximately 30% of the oligogalacturonide) occurs in approximately 25 min. The unbound dodecagalacturonic acid-[3H]galactitol is rapidly (half-life, 30 min) fragmented to smaller, biologically inactive fragments by a polygalacturonase present in the growth medium. In contrast, the wall-bound dodecagalacturonic acid-[3H]galactitol is not degraded for at least 150 min. However, the kinetics, amplitude and duration of oligogalacturonide-induced ion fluxes are not correlated with the rate at which oligogalacturonides are converted to biologically inactive fragments. We propose that the transient nature of the oligogalacturonide-induced responses is likely to result from a temporary desensitization of the plant cells to the bioactive oligogalacturonides. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Plant Journal is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1046/j.1365-313X.1998.00297.x
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      – Code: eng
        Text: English
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        PageCount: 7
        StartPage: 305
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      – SubjectFull: Galactans
        Type: general
      – SubjectFull: Tobacco
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      – SubjectFull: Cytology
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              Text: Nov98
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              Y: 1998
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