Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol) as an exogenous chain transfer agent.
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| Title: | Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol) as an exogenous chain transfer agent. |
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| Authors: | Tomizawa, Satoshi1, Saito, Yuta1, Hyakutake, Manami1, Nakamura, Yoshiyuki2, Abe, Hideki3, Tsuge, Takeharu1 tsuge.t.aa@m.titech.ac.jp |
| Source: | Applied Microbiology & Biotechnology. Jul2010, Vol. 87 Issue 4, p1427-1435. 9p. 1 Diagram, 6 Charts, 2 Graphs. |
| Subjects: | Poly-beta-hydroxyalkanoates, Catalysis, Polymerization, Ethylene glycol, Escherichia coli, Polymers, Molecular weights, Nuclear magnetic resonance, Polyhydroxyalkanoates |
| Abstract: | Polyhydroxyalkanoate (PHA) synthases catalyze chain transfer (CT) reaction after polymerization reaction of PHA by transferring PHA chain from PHA synthase to a CT agent, resulting in covalent bonding of CT agent to PHA chain at the carboxyl end. Previous studies have shown that poly(ethylene glycol) (PEG) is an effective exogenous CT agent. This study aimed to compare the effects of PEG on CT reaction during poly[( R)-3-hydroxybutyrate] [P(3HB)] synthesis by using six PHA synthases in Escherichia coli JM109. The synthesized P(3HB) polymers were characterized in terms of molecular weight and end-group structure. Supplementation of PEG to the culture medium reduced P(3HB) molecular weights by up to 96% due to PEG-induced CT reaction. The P(3HB) polymers were subjected to 1H NMR analysis to confirm the formation of a covalent bond between PEG and P(3HB) chain at the carboxyl end. This study revealed the reactivity of PHA synthases to PEG with respect to CT reaction in E. coli. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 51708948 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol) as an exogenous chain transfer agent. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Tomizawa%2C+Satoshi%22">Tomizawa, Satoshi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Saito%2C+Yuta%22">Saito, Yuta</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hyakutake%2C+Manami%22">Hyakutake, Manami</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Nakamura%2C+Yoshiyuki%22">Nakamura, Yoshiyuki</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Abe%2C+Hideki%22">Abe, Hideki</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Tsuge%2C+Takeharu%22">Tsuge, Takeharu</searchLink><relatesTo>1</relatesTo><i> tsuge.t.aa@m.titech.ac.jp</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Jul2010, Vol. 87 Issue 4, p1427-1435. 9p. 1 Diagram, 6 Charts, 2 Graphs. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Poly-beta-hydroxyalkanoates%22">Poly-beta-hydroxyalkanoates</searchLink><br /><searchLink fieldCode="DE" term="%22Catalysis%22">Catalysis</searchLink><br /><searchLink fieldCode="DE" term="%22Polymerization%22">Polymerization</searchLink><br /><searchLink fieldCode="DE" term="%22Ethylene+glycol%22">Ethylene glycol</searchLink><br /><searchLink fieldCode="DE" term="%22Escherichia+coli%22">Escherichia coli</searchLink><br /><searchLink fieldCode="DE" term="%22Polymers%22">Polymers</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+weights%22">Molecular weights</searchLink><br /><searchLink fieldCode="DE" term="%22Nuclear+magnetic+resonance%22">Nuclear magnetic resonance</searchLink><br /><searchLink fieldCode="DE" term="%22Polyhydroxyalkanoates%22">Polyhydroxyalkanoates</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Polyhydroxyalkanoate (PHA) synthases catalyze chain transfer (CT) reaction after polymerization reaction of PHA by transferring PHA chain from PHA synthase to a CT agent, resulting in covalent bonding of CT agent to PHA chain at the carboxyl end. Previous studies have shown that poly(ethylene glycol) (PEG) is an effective exogenous CT agent. This study aimed to compare the effects of PEG on CT reaction during poly[( R)-3-hydroxybutyrate] [P(3HB)] synthesis by using six PHA synthases in Escherichia coli JM109. The synthesized P(3HB) polymers were characterized in terms of molecular weight and end-group structure. Supplementation of PEG to the culture medium reduced P(3HB) molecular weights by up to 96% due to PEG-induced CT reaction. The P(3HB) polymers were subjected to 1H NMR analysis to confirm the formation of a covalent bond between PEG and P(3HB) chain at the carboxyl end. This study revealed the reactivity of PHA synthases to PEG with respect to CT reaction in E. coli. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-010-2601-8 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 9 StartPage: 1427 Subjects: – SubjectFull: Poly-beta-hydroxyalkanoates Type: general – SubjectFull: Catalysis Type: general – SubjectFull: Polymerization Type: general – SubjectFull: Ethylene glycol Type: general – SubjectFull: Escherichia coli Type: general – SubjectFull: Polymers Type: general – SubjectFull: Molecular weights Type: general – SubjectFull: Nuclear magnetic resonance Type: general – SubjectFull: Polyhydroxyalkanoates Type: general Titles: – TitleFull: Chain transfer reaction catalyzed by various polyhydroxyalkanoate synthases with poly(ethylene glycol) as an exogenous chain transfer agent. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Tomizawa, Satoshi – PersonEntity: Name: NameFull: Saito, Yuta – PersonEntity: Name: NameFull: Hyakutake, Manami – PersonEntity: Name: NameFull: Nakamura, Yoshiyuki – PersonEntity: Name: NameFull: Abe, Hideki – PersonEntity: Name: NameFull: Tsuge, Takeharu IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 07 Text: Jul2010 Type: published Y: 2010 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 87 – Type: issue Value: 4 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
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