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Hempseed protein derived antioxidative peptides: Purification, identification and protection from hydrogen peroxide-induced apoptosis in PC12 cells

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Title: Hempseed protein derived antioxidative peptides: Purification, identification and protection from hydrogen peroxide-induced apoptosis in PC12 cells
Authors: Lu, Rong-Rong1 lurr@jiangnan.edu.cn, Qian, Ping2, Sun, Zhen1, Zhou, Xu-Hui1, Chen, Tian-Peng2, He, Jin-Feng2, Zhang, Hua2, Wu, Jianping3
Source: Food Chemistry. Dec2010, Vol. 123 Issue 4, p1210-1218. 9p.
Subjects: Antioxidants, Hemp, Apoptosis, Oxidative stress, Cell lines, Hydrogen peroxide, Matrix-assisted laser desorption-ionization, Time-of-flight mass spectrometry, Therapeutics
Abstract: Abstract: Hempseeds have not been utilised to any significant extent by the industrial food markets. A novel antioxidant peptide derived from hempseed by-product was investigated. Hempseed protein isolate was prepared by alkaline extraction and subsequent isoelectric precipitation. Its enzymatic hydrolysate, obtained by Alcalase® treatment, was subjected to DA201-C macroporous absorption resin, with simultaneous desalting and concentrating of hydrophobic fragments with improved free radical-scavenging activities. The active fraction was further separated by gel filtration and reversed-phase high performance liquid chromatography to obtain two purified peptides; the peptides were characterised as NHAV and HVRETALV, by MALDI-TOF/TOF MS/MS. The protective effects of the purified peptides on hydrogen peroxide-induced cell apoptosis were investigated in rat pheochromocytoma line PC12 cells. The peptides, at a concentration of 10μg/ml, possessed protective effects (P <0.05) against cell death and oxidative apoptosis. The findings are significant for the discovery and development of natural antioxidants from hempseed by-products. [Copyright &y& Elsevier]
Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Hempseed protein derived antioxidative peptides: Purification, identification and protection from hydrogen peroxide-induced apoptosis in PC12 cells
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  Data: &lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Lu%2C+Rong-Rong%22&quot;&gt;Lu, Rong-Rong&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;i&gt; lurr@jiangnan.edu.cn&lt;/i&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Qian%2C+Ping%22&quot;&gt;Qian, Ping&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Sun%2C+Zhen%22&quot;&gt;Sun, Zhen&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zhou%2C+Xu-Hui%22&quot;&gt;Zhou, Xu-Hui&lt;/searchLink&gt;&lt;relatesTo&gt;1&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Chen%2C+Tian-Peng%22&quot;&gt;Chen, Tian-Peng&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22He%2C+Jin-Feng%22&quot;&gt;He, Jin-Feng&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Zhang%2C+Hua%22&quot;&gt;Zhang, Hua&lt;/searchLink&gt;&lt;relatesTo&gt;2&lt;/relatesTo&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;AR&quot; term=&quot;%22Wu%2C+Jianping%22&quot;&gt;Wu, Jianping&lt;/searchLink&gt;&lt;relatesTo&gt;3&lt;/relatesTo&gt;
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  Data: &lt;searchLink fieldCode=&quot;JN&quot; term=&quot;%22Food+Chemistry%22&quot;&gt;Food Chemistry&lt;/searchLink&gt;. Dec2010, Vol. 123 Issue 4, p1210-1218. 9p.
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  Data: &lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Antioxidants%22&quot;&gt;Antioxidants&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Hemp%22&quot;&gt;Hemp&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Apoptosis%22&quot;&gt;Apoptosis&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Oxidative+stress%22&quot;&gt;Oxidative stress&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Cell+lines%22&quot;&gt;Cell lines&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Hydrogen+peroxide%22&quot;&gt;Hydrogen peroxide&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Matrix-assisted+laser+desorption-ionization%22&quot;&gt;Matrix-assisted laser desorption-ionization&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Time-of-flight+mass+spectrometry%22&quot;&gt;Time-of-flight mass spectrometry&lt;/searchLink&gt;&lt;br /&gt;&lt;searchLink fieldCode=&quot;DE&quot; term=&quot;%22Therapeutics%22&quot;&gt;Therapeutics&lt;/searchLink&gt;
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  Data: Abstract: Hempseeds have not been utilised to any significant extent by the industrial food markets. A novel antioxidant peptide derived from hempseed by-product was investigated. Hempseed protein isolate was prepared by alkaline extraction and subsequent isoelectric precipitation. Its enzymatic hydrolysate, obtained by Alcalase&#174; treatment, was subjected to DA201-C macroporous absorption resin, with simultaneous desalting and concentrating of hydrophobic fragments with improved free radical-scavenging activities. The active fraction was further separated by gel filtration and reversed-phase high performance liquid chromatography to obtain two purified peptides; the peptides were characterised as NHAV and HVRETALV, by MALDI-TOF/TOF MS/MS. The protective effects of the purified peptides on hydrogen peroxide-induced cell apoptosis were investigated in rat pheochromocytoma line PC12 cells. The peptides, at a concentration of 10μg/ml, possessed protective effects (P &lt;0.05) against cell death and oxidative apoptosis. The findings are significant for the discovery and development of natural antioxidants from hempseed by-products. [Copyright &amp;y&amp; Elsevier]
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  Data: &lt;i&gt;Copyright of Food Chemistry is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder&#39;s express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.&lt;/i&gt; (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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    Identifiers:
      – Type: doi
        Value: 10.1016/j.foodchem.2010.05.089
    Languages:
      – Code: eng
        Text: English
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      Pagination:
        PageCount: 9
        StartPage: 1210
    Subjects:
      – SubjectFull: Antioxidants
        Type: general
      – SubjectFull: Hemp
        Type: general
      – SubjectFull: Apoptosis
        Type: general
      – SubjectFull: Oxidative stress
        Type: general
      – SubjectFull: Cell lines
        Type: general
      – SubjectFull: Hydrogen peroxide
        Type: general
      – SubjectFull: Matrix-assisted laser desorption-ionization
        Type: general
      – SubjectFull: Time-of-flight mass spectrometry
        Type: general
      – SubjectFull: Therapeutics
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      – TitleFull: Hempseed protein derived antioxidative peptides: Purification, identification and protection from hydrogen peroxide-induced apoptosis in PC12 cells
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            NameFull: Lu, Rong-Rong
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            NameFull: Qian, Ping
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            NameFull: Sun, Zhen
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              M: 12
              Text: Dec2010
              Type: published
              Y: 2010
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              Value: 123
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            – TitleFull: Food Chemistry
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