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Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2

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Title: Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2
Authors: Wu, Yi-Rui1 oxwyr@tom.com, Luo, Zhu-Hua1,2, Kwok-Kei Chow, R.1, Vrijmoed, L.L.P.1 bhlilian@cityu.edu.hk
Source: Bioresource Technology. Dec2010, Vol. 101 Issue 24, p9772-9777. 6p.
Subjects: Polycyclic aromatic hydrocarbons, Mangrove plants, Sedimentation & deposition, Acetone, Heavy metals, Biotechnology, Molecular weights, Endoglycosidases
Abstract: Abstract: An extracellular laccase was purified from the culture medium of the non-white rot, anthracene-degrading fungal strain Fusarium solani MAS2. Both native PAGE and SDS–PAGE revealed one single band corresponding to a molecular weight of about 72kDa. Treatment with endoglycosidase H reduced the molecular weight by 12%. The purified laccase maintained stable at pH 3–11 and up to 50°C. The highest activity was detected at pH 3.0 and at 70°C. The enzyme retained 46.2–97.2% of it activity in the presence of 20mM Pb2+, Ni2+, Cr3+, and its activity was enhanced in the presence of 20mM Hg2+. The laccase retained more than 50% of its activity in the presence of 5% acetone, acetonitrile, dimethyl sulphoxide (DMSO), ethanol and methanol. The kinetic constants (K m and k cat) showed that 2,6-dimethoxyphenol (DMOP) and 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) were the more effective substrates rather than catechol and guaiacol. The novel properties of this laccase suggest its potential for biotechnological and environmental applications. [ABSTRACT FROM AUTHOR]
Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 53388931
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PubType: Academic Journal
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  Data: Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2
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  Data: <searchLink fieldCode="AR" term="%22Wu%2C+Yi-Rui%22">Wu, Yi-Rui</searchLink><relatesTo>1</relatesTo><i> oxwyr@tom.com</i><br /><searchLink fieldCode="AR" term="%22Luo%2C+Zhu-Hua%22">Luo, Zhu-Hua</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kwok-Kei+Chow%2C+R%2E%22">Kwok-Kei Chow, R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Vrijmoed%2C+L%2EL%2EP%2E%22">Vrijmoed, L.L.P.</searchLink><relatesTo>1</relatesTo><i> bhlilian@cityu.edu.hk</i>
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  Data: <searchLink fieldCode="JN" term="%22Bioresource+Technology%22">Bioresource Technology</searchLink>. Dec2010, Vol. 101 Issue 24, p9772-9777. 6p.
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  Data: <searchLink fieldCode="DE" term="%22Polycyclic+aromatic+hydrocarbons%22">Polycyclic aromatic hydrocarbons</searchLink><br /><searchLink fieldCode="DE" term="%22Mangrove+plants%22">Mangrove plants</searchLink><br /><searchLink fieldCode="DE" term="%22Sedimentation+%26+deposition%22">Sedimentation & deposition</searchLink><br /><searchLink fieldCode="DE" term="%22Acetone%22">Acetone</searchLink><br /><searchLink fieldCode="DE" term="%22Heavy+metals%22">Heavy metals</searchLink><br /><searchLink fieldCode="DE" term="%22Biotechnology%22">Biotechnology</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+weights%22">Molecular weights</searchLink><br /><searchLink fieldCode="DE" term="%22Endoglycosidases%22">Endoglycosidases</searchLink>
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  Data: Abstract: An extracellular laccase was purified from the culture medium of the non-white rot, anthracene-degrading fungal strain Fusarium solani MAS2. Both native PAGE and SDS–PAGE revealed one single band corresponding to a molecular weight of about 72kDa. Treatment with endoglycosidase H reduced the molecular weight by 12%. The purified laccase maintained stable at pH 3–11 and up to 50°C. The highest activity was detected at pH 3.0 and at 70°C. The enzyme retained 46.2–97.2% of it activity in the presence of 20mM Pb2+, Ni2+, Cr3+, and its activity was enhanced in the presence of 20mM Hg2+. The laccase retained more than 50% of its activity in the presence of 5% acetone, acetonitrile, dimethyl sulphoxide (DMSO), ethanol and methanol. The kinetic constants (K m and k cat) showed that 2,6-dimethoxyphenol (DMOP) and 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) diammonium salt (ABTS) were the more effective substrates rather than catechol and guaiacol. The novel properties of this laccase suggest its potential for biotechnological and environmental applications. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Bioresource Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.biortech.2010.07.091
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      – SubjectFull: Molecular weights
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      – TitleFull: Purification and characterization of an extracellular laccase from the anthracene-degrading fungus Fusarium solani MAS2
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              Text: Dec2010
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