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The added value of rabies virus as a retrograde tracer when combined with dual anterograde tract-tracing

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Title: The added value of rabies virus as a retrograde tracer when combined with dual anterograde tract-tracing
Authors: López, Iciar P.1, Salin, Pascal2, Kachidian, Philippe2, Barroso-Chinea, Pedro1, Rico, Alberto J.1, Gómez-Bautista, Virginia1, Conte-Perales, Lorena1, Coulon, Patrice3, Goff, Lydia Kerkerian-Le2, Lanciego, José L.1 jlanciego@unav.es
Source: Journal of Neuroscience Methods. Dec2010, Vol. 194 Issue 1, p21-27. 7p.
Subjects: Rabies virus, Axonal transport, Neurons, Nucleoproteins, Viral antibodies, Cytoplasm, Phosphoproteins, Immunofluorescence, Confocal microscopy
Abstract: Abstract: Rabies virus (RV) has widely been used as a trans-synaptic retrograde tracer to analyze chains of connected neurons. The use of antibodies directed against the viral nucleoprotein enables viral nucleocapsids to be visualized within the cell soma, as well as within the thickest main dendrites. However, through this approach it is often difficult to accurately define post-synaptic elements (thin dendrites and/or dendritic spines). This limitation can now easily been circumvented by taking advantage of antibodies directed against a soluble viral phosphoprotein that spreads throughout the cytoplasm of the infected neuron, thereby producing Golgi-like immunofluorescent labeling of first-order projection neurons that are infected with RV. Furthermore, when combined with anterograde tracers such as Phaseolus vulgaris-leucoagglutinin (PHA-L) and biotinylated dextran amine (BDA), this procedure to detect RV facilitates the accurate visualization of both the pre- and post-synaptic elements. Finally, this method of viral detection is sufficiently sensitive to detect weakly labeled second-order neurons, which can then be further characterized neurochemically. Several examples are provided to illustrate why retrograde trans-synaptic tracing using RV can be regarded as an important breakthrough in the analysis of brain circuits, providing an unprecedented level of resolution. [ABSTRACT FROM AUTHOR]
Copyright of Journal of Neuroscience Methods is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 55498332
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  Data: The added value of rabies virus as a retrograde tracer when combined with dual anterograde tract-tracing
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  Data: <searchLink fieldCode="AR" term="%22López%2C+Iciar+P%2E%22">López, Iciar P.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Salin%2C+Pascal%22">Salin, Pascal</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kachidian%2C+Philippe%22">Kachidian, Philippe</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Barroso-Chinea%2C+Pedro%22">Barroso-Chinea, Pedro</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Rico%2C+Alberto+J%2E%22">Rico, Alberto J.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Gómez-Bautista%2C+Virginia%22">Gómez-Bautista, Virginia</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Conte-Perales%2C+Lorena%22">Conte-Perales, Lorena</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Coulon%2C+Patrice%22">Coulon, Patrice</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Goff%2C+Lydia+Kerkerian-Le%22">Goff, Lydia Kerkerian-Le</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Lanciego%2C+José+L%2E%22">Lanciego, José L.</searchLink><relatesTo>1</relatesTo><i> jlanciego@unav.es</i>
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  Data: <searchLink fieldCode="DE" term="%22Rabies+virus%22">Rabies virus</searchLink><br /><searchLink fieldCode="DE" term="%22Axonal+transport%22">Axonal transport</searchLink><br /><searchLink fieldCode="DE" term="%22Neurons%22">Neurons</searchLink><br /><searchLink fieldCode="DE" term="%22Nucleoproteins%22">Nucleoproteins</searchLink><br /><searchLink fieldCode="DE" term="%22Viral+antibodies%22">Viral antibodies</searchLink><br /><searchLink fieldCode="DE" term="%22Cytoplasm%22">Cytoplasm</searchLink><br /><searchLink fieldCode="DE" term="%22Phosphoproteins%22">Phosphoproteins</searchLink><br /><searchLink fieldCode="DE" term="%22Immunofluorescence%22">Immunofluorescence</searchLink><br /><searchLink fieldCode="DE" term="%22Confocal+microscopy%22">Confocal microscopy</searchLink>
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  Label: Abstract
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  Data: Abstract: Rabies virus (RV) has widely been used as a trans-synaptic retrograde tracer to analyze chains of connected neurons. The use of antibodies directed against the viral nucleoprotein enables viral nucleocapsids to be visualized within the cell soma, as well as within the thickest main dendrites. However, through this approach it is often difficult to accurately define post-synaptic elements (thin dendrites and/or dendritic spines). This limitation can now easily been circumvented by taking advantage of antibodies directed against a soluble viral phosphoprotein that spreads throughout the cytoplasm of the infected neuron, thereby producing Golgi-like immunofluorescent labeling of first-order projection neurons that are infected with RV. Furthermore, when combined with anterograde tracers such as Phaseolus vulgaris-leucoagglutinin (PHA-L) and biotinylated dextran amine (BDA), this procedure to detect RV facilitates the accurate visualization of both the pre- and post-synaptic elements. Finally, this method of viral detection is sufficiently sensitive to detect weakly labeled second-order neurons, which can then be further characterized neurochemically. Several examples are provided to illustrate why retrograde trans-synaptic tracing using RV can be regarded as an important breakthrough in the analysis of brain circuits, providing an unprecedented level of resolution. [ABSTRACT FROM AUTHOR]
– Name: AbstractSuppliedCopyright
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  Group: Ab
  Data: <i>Copyright of Journal of Neuroscience Methods is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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      – Type: doi
        Value: 10.1016/j.jneumeth.2010.01.015
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      – Code: eng
        Text: English
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        PageCount: 7
        StartPage: 21
    Subjects:
      – SubjectFull: Rabies virus
        Type: general
      – SubjectFull: Axonal transport
        Type: general
      – SubjectFull: Neurons
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      – SubjectFull: Nucleoproteins
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      – SubjectFull: Viral antibodies
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      – SubjectFull: Cytoplasm
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      – SubjectFull: Phosphoproteins
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      – SubjectFull: Immunofluorescence
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      – SubjectFull: Confocal microscopy
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      – TitleFull: The added value of rabies virus as a retrograde tracer when combined with dual anterograde tract-tracing
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              Text: Dec2010
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