Formation of new polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer in Burkholderia sp.
Saved in:
| Title: | Formation of new polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer in Burkholderia sp. |
|---|---|
| Authors: | Nyok-Sean Lau1, Tsuge, Takeharu2, Sudesh, Kumar1 ksudesh@usm.my |
| Source: | Applied Microbiology & Biotechnology. Mar2011, Vol. 89 Issue 5, p1599-1609. 11p. |
| Subjects: | Poly-beta-hydroxyalkanoates, Monomers, Burkholderia, Aeromonas, Fructose, Copolymers, Nuclear magnetic resonance, Fourier transform infrared spectroscopy |
| Abstract: | Burkholderia sp. synthase has been shown to polymerize 3-hydroxybutyrate (3HB), 3-hydroxyvalerate, and 3-hydroxy-4-pentenoic acid monomers. This study was carried out to evaluate the ability of Burkholderia sp. USM (JCM 15050) and its transformant harboring the polyhydroxyalkanoate (PHA) synthase gene of Aeromonas caviae to incorporate the newly reported 3-hydroxy-4-methylvalerate (3H4MV) monomer. Various culture parameters such as concentrations of nutrient rich medium, fructose and 4-methylvaleric acid as well as harvesting time were manipulated to produce P(3HB- co-3H4MV) with different 3H4MV compositions. The structural properties of PHA containing 3H4MV monomer were investigated by using nuclear magnetic resonance and Fourier transform infrared spectroscopy (FTIR). The relative intensities of the bands at 1,183 and 1,228 cm in the FTIR spectra enabled the rapid detection and differentiation of P(3HB- co-3H4MV) from other types of PHA. In addition, the presence of 3H4MV units in the copolymer was found to considerably lower the melting temperature and enthalpy of fusion values compared with poly(3-hydroxybutyrate) (P(3HB)). The copolymer exhibited higher thermo-degradation temperature but similar molecular weight and polydispersity compared with P(3HB). [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Links: – Type: pdflink Text: Availability: 0 |
|---|---|
| Header | DbId: egs DbLabel: Engineering Source An: 57964423 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
| IllustrationInfo | |
| Items | – Name: Title Label: Title Group: Ti Data: Formation of new polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer in Burkholderia sp. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Nyok-Sean+Lau%22">Nyok-Sean Lau</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Tsuge%2C+Takeharu%22">Tsuge, Takeharu</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Sudesh%2C+Kumar%22">Sudesh, Kumar</searchLink><relatesTo>1</relatesTo><i> ksudesh@usm.my</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Mar2011, Vol. 89 Issue 5, p1599-1609. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Poly-beta-hydroxyalkanoates%22">Poly-beta-hydroxyalkanoates</searchLink><br /><searchLink fieldCode="DE" term="%22Monomers%22">Monomers</searchLink><br /><searchLink fieldCode="DE" term="%22Burkholderia%22">Burkholderia</searchLink><br /><searchLink fieldCode="DE" term="%22Aeromonas%22">Aeromonas</searchLink><br /><searchLink fieldCode="DE" term="%22Fructose%22">Fructose</searchLink><br /><searchLink fieldCode="DE" term="%22Copolymers%22">Copolymers</searchLink><br /><searchLink fieldCode="DE" term="%22Nuclear+magnetic+resonance%22">Nuclear magnetic resonance</searchLink><br /><searchLink fieldCode="DE" term="%22Fourier+transform+infrared+spectroscopy%22">Fourier transform infrared spectroscopy</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Burkholderia sp. synthase has been shown to polymerize 3-hydroxybutyrate (3HB), 3-hydroxyvalerate, and 3-hydroxy-4-pentenoic acid monomers. This study was carried out to evaluate the ability of Burkholderia sp. USM (JCM 15050) and its transformant harboring the polyhydroxyalkanoate (PHA) synthase gene of Aeromonas caviae to incorporate the newly reported 3-hydroxy-4-methylvalerate (3H4MV) monomer. Various culture parameters such as concentrations of nutrient rich medium, fructose and 4-methylvaleric acid as well as harvesting time were manipulated to produce P(3HB- co-3H4MV) with different 3H4MV compositions. The structural properties of PHA containing 3H4MV monomer were investigated by using nuclear magnetic resonance and Fourier transform infrared spectroscopy (FTIR). The relative intensities of the bands at 1,183 and 1,228 cm in the FTIR spectra enabled the rapid detection and differentiation of P(3HB- co-3H4MV) from other types of PHA. In addition, the presence of 3H4MV units in the copolymer was found to considerably lower the melting temperature and enthalpy of fusion values compared with poly(3-hydroxybutyrate) (P(3HB)). The copolymer exhibited higher thermo-degradation temperature but similar molecular weight and polydispersity compared with P(3HB). [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
| PLink | https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=57964423 |
| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-011-3097-6 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 1599 Subjects: – SubjectFull: Poly-beta-hydroxyalkanoates Type: general – SubjectFull: Monomers Type: general – SubjectFull: Burkholderia Type: general – SubjectFull: Aeromonas Type: general – SubjectFull: Fructose Type: general – SubjectFull: Copolymers Type: general – SubjectFull: Nuclear magnetic resonance Type: general – SubjectFull: Fourier transform infrared spectroscopy Type: general Titles: – TitleFull: Formation of new polyhydroxyalkanoate containing 3-hydroxy-4-methylvalerate monomer in Burkholderia sp. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Nyok-Sean Lau – PersonEntity: Name: NameFull: Tsuge, Takeharu – PersonEntity: Name: NameFull: Sudesh, Kumar IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 03 Text: Mar2011 Type: published Y: 2011 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 89 – Type: issue Value: 5 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
