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Separation of sight-protecting zeaxanthin from Nannochloropsis oculata by using supercritical fluids extraction coupled with elution chromatography

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Title: Separation of sight-protecting zeaxanthin from Nannochloropsis oculata by using supercritical fluids extraction coupled with elution chromatography
Authors: Liau, Bing-Chung1, Hong, Siang-En1, Chang, Li-Pin1, Shen, Chun-Tin1, Li, Yu-Chun1, Wu, Ying-Ping1, Jong, Ting-Ting2, Shieh, Chwen-Jen3, Hsu, Shih-Lan4, Chang, Chieh-Ming J.1 cmchang@dragon.nchu.edu.tw
Source: Separation & Purification Technology. Mar2011, Vol. 78 Issue 1, p1-8. 8p.
Subjects: Carotenoids, Separation (Technology), Supercritical fluids, Extraction (Chemistry), Chromatographic analysis, Microalgae, Antioxidants, Experiments
Abstract: Abstract: Microalgae is a potent source of carotenoids and is regarded as functional foods consumed by people worldwide. In this study, supercritical fluid extraction coupled with elution chromatography was employed to recover six carotenoids including zeaxanthin from Nannochloropsis oculata and extraction efficiency of zeaxanthin was determined. The amount of the zeaxanthin (400mg/g) in the elution fraction increased more than twenty folds from that of the SFE extract (13.17mg/g). The extracts were subsequently subjected to tests of antioxidant capacities in 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay, 2-2′-azino-bis (3-ethylbenzo- thiazoline-6-sulfonic acid (ABTS)) assay and adult retinal pigment epithelium-19 (ARPE-19) cells. Experimental results demonstrate that other components than zeaxanthin may play a crucial role in the anti-oxidative ability of the extracts from N. oculata. This work provides a useful method to recover bioactive anti-oxidative zeaxanthin from microalgae of N. oculata by using modified supercritical fluids extraction. [Copyright &y& Elsevier]
Copyright of Separation & Purification Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 59187658
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  Data: Separation of sight-protecting zeaxanthin from Nannochloropsis oculata by using supercritical fluids extraction coupled with elution chromatography
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  Data: <searchLink fieldCode="AR" term="%22Liau%2C+Bing-Chung%22">Liau, Bing-Chung</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hong%2C+Siang-En%22">Hong, Siang-En</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Chang%2C+Li-Pin%22">Chang, Li-Pin</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Shen%2C+Chun-Tin%22">Shen, Chun-Tin</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Yu-Chun%22">Li, Yu-Chun</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wu%2C+Ying-Ping%22">Wu, Ying-Ping</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Jong%2C+Ting-Ting%22">Jong, Ting-Ting</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Shieh%2C+Chwen-Jen%22">Shieh, Chwen-Jen</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Hsu%2C+Shih-Lan%22">Hsu, Shih-Lan</searchLink><relatesTo>4</relatesTo><br /><searchLink fieldCode="AR" term="%22Chang%2C+Chieh-Ming+J%2E%22">Chang, Chieh-Ming J.</searchLink><relatesTo>1</relatesTo><i> cmchang@dragon.nchu.edu.tw</i>
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  Data: Abstract: Microalgae is a potent source of carotenoids and is regarded as functional foods consumed by people worldwide. In this study, supercritical fluid extraction coupled with elution chromatography was employed to recover six carotenoids including zeaxanthin from Nannochloropsis oculata and extraction efficiency of zeaxanthin was determined. The amount of the zeaxanthin (400mg/g) in the elution fraction increased more than twenty folds from that of the SFE extract (13.17mg/g). The extracts were subsequently subjected to tests of antioxidant capacities in 2,2-diphenyl-2-picrylhydrazyl hydrate (DPPH) assay, 2-2′-azino-bis (3-ethylbenzo- thiazoline-6-sulfonic acid (ABTS)) assay and adult retinal pigment epithelium-19 (ARPE-19) cells. Experimental results demonstrate that other components than zeaxanthin may play a crucial role in the anti-oxidative ability of the extracts from N. oculata. This work provides a useful method to recover bioactive anti-oxidative zeaxanthin from microalgae of N. oculata by using modified supercritical fluids extraction. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Separation & Purification Technology is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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        Value: 10.1016/j.seppur.2011.01.008
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      – Code: eng
        Text: English
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      – TitleFull: Separation of sight-protecting zeaxanthin from Nannochloropsis oculata by using supercritical fluids extraction coupled with elution chromatography
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