Microbial production of polyhydroxyalkanoate block copolymer by recombinant Pseudomonas putida.
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| Title: | Microbial production of polyhydroxyalkanoate block copolymer by recombinant Pseudomonas putida. |
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| Authors: | Shi Yan Li1, Cui Ling Dong1, Shen Yu Wang1, Hai Mu Ye2, Guo-Qiang Chen3 chengq@mail.tsinghua.edu.cn |
| Source: | Applied Microbiology & Biotechnology. Apr2011, Vol. 90 Issue 2, p659-669. 11p. |
| Subjects: | Poly-beta-hydroxyalkanoates, Pseudomonas, Block copolymers, Glass transition temperature, Calorimetry, Polyhydroxyalkanoates |
| Abstract: | Polyhydroxyalkanoate (PHA) synthesis genes phaPCJ cloned from Aeromonas caviae were transformed into Pseudomonas putida KTOY06ΔC, a mutant of P. putida KT2442, resulting in the ability of the recombinant P. putida KTOY06ΔC ( phaPCJ) to produce a short-chain-length and medium-chain-length PHA block copolymer consisting of poly-3-hydroxybutyrate (PHB) as one block and random copolymer of 3-hydroxyvalerate (3HV) and 3-hydroxyheptanoate (3HHp) as another block. The novel block polymer was studied by differential scanning calorimetry (DSC), nuclear magnetic resonance, and rheology measurements. DSC studies showed the polymer to possess two glass transition temperatures ( T), one melting temperature ( T) and one cool crystallization temperature ( T). Rheology studies clearly indicated a polymer chain re-arrangement in the copolymer; these studies confirmed the polymer to be a block copolymer, with over 70 mol% homopolymer (PHB) of 3-hydroxybutyrate (3HB) as one block and around 30 mol% random copolymers of 3HV and 3HHp as the second block. The block copolymer was shown to have the highest tensile strength and Young's modulus compared with a random copolymer with similar ratio and a blend of homopolymers PHB and PHVHHp with similar ratio. Compared with other commercially available PHA including PHB, PHBV, PHBHHx, and P3HB4HB, the short-chain- and medium-chain-length block copolymer PHB- b-PHVHHp showed differences in terms of mechanical properties and should draw more attentions from the PHA research community. [ABSTRACT FROM AUTHOR] |
| Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: Microbial production of polyhydroxyalkanoate block copolymer by recombinant Pseudomonas putida. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Shi+Yan+Li%22">Shi Yan Li</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Cui+Ling+Dong%22">Cui Ling Dong</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Shen+Yu+Wang%22">Shen Yu Wang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Hai+Mu+Ye%22">Hai Mu Ye</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Guo-Qiang+Chen%22">Guo-Qiang Chen</searchLink><relatesTo>3</relatesTo><i> chengq@mail.tsinghua.edu.cn</i> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Applied+Microbiology+%26+Biotechnology%22">Applied Microbiology & Biotechnology</searchLink>. Apr2011, Vol. 90 Issue 2, p659-669. 11p. – Name: Subject Label: Subjects Group: Su Data: <searchLink fieldCode="DE" term="%22Poly-beta-hydroxyalkanoates%22">Poly-beta-hydroxyalkanoates</searchLink><br /><searchLink fieldCode="DE" term="%22Pseudomonas%22">Pseudomonas</searchLink><br /><searchLink fieldCode="DE" term="%22Block+copolymers%22">Block copolymers</searchLink><br /><searchLink fieldCode="DE" term="%22Glass+transition+temperature%22">Glass transition temperature</searchLink><br /><searchLink fieldCode="DE" term="%22Calorimetry%22">Calorimetry</searchLink><br /><searchLink fieldCode="DE" term="%22Polyhydroxyalkanoates%22">Polyhydroxyalkanoates</searchLink> – Name: Abstract Label: Abstract Group: Ab Data: Polyhydroxyalkanoate (PHA) synthesis genes phaPCJ cloned from Aeromonas caviae were transformed into Pseudomonas putida KTOY06ΔC, a mutant of P. putida KT2442, resulting in the ability of the recombinant P. putida KTOY06ΔC ( phaPCJ) to produce a short-chain-length and medium-chain-length PHA block copolymer consisting of poly-3-hydroxybutyrate (PHB) as one block and random copolymer of 3-hydroxyvalerate (3HV) and 3-hydroxyheptanoate (3HHp) as another block. The novel block polymer was studied by differential scanning calorimetry (DSC), nuclear magnetic resonance, and rheology measurements. DSC studies showed the polymer to possess two glass transition temperatures ( T), one melting temperature ( T) and one cool crystallization temperature ( T). Rheology studies clearly indicated a polymer chain re-arrangement in the copolymer; these studies confirmed the polymer to be a block copolymer, with over 70 mol% homopolymer (PHB) of 3-hydroxybutyrate (3HB) as one block and around 30 mol% random copolymers of 3HV and 3HHp as the second block. The block copolymer was shown to have the highest tensile strength and Young's modulus compared with a random copolymer with similar ratio and a blend of homopolymers PHB and PHVHHp with similar ratio. Compared with other commercially available PHA including PHB, PHBV, PHBHHx, and P3HB4HB, the short-chain- and medium-chain-length block copolymer PHB- b-PHVHHp showed differences in terms of mechanical properties and should draw more attentions from the PHA research community. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Applied Microbiology & Biotechnology is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s00253-010-3069-2 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 659 Subjects: – SubjectFull: Poly-beta-hydroxyalkanoates Type: general – SubjectFull: Pseudomonas Type: general – SubjectFull: Block copolymers Type: general – SubjectFull: Glass transition temperature Type: general – SubjectFull: Calorimetry Type: general – SubjectFull: Polyhydroxyalkanoates Type: general Titles: – TitleFull: Microbial production of polyhydroxyalkanoate block copolymer by recombinant Pseudomonas putida. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Shi Yan Li – PersonEntity: Name: NameFull: Cui Ling Dong – PersonEntity: Name: NameFull: Shen Yu Wang – PersonEntity: Name: NameFull: Hai Mu Ye – PersonEntity: Name: NameFull: Guo-Qiang Chen IsPartOfRelationships: – BibEntity: Dates: – D: 15 M: 04 Text: Apr2011 Type: published Y: 2011 Identifiers: – Type: issn-print Value: 01757598 Numbering: – Type: volume Value: 90 – Type: issue Value: 2 Titles: – TitleFull: Applied Microbiology & Biotechnology Type: main |
| ResultId | 1 |
