View in EDS

New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry

Saved in:
Bibliographic Details
Title: New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry
Authors: Ziganshin, Rustam rustam.ziganshin@gmail.com, Arapidi, Georgii, Azarkin, Igor, Zaryadieva, Elena, Alexeev, Dmitry, Govorun, Vadim, Ivanov, Vadim
Source: Journal of Proteomics. May2011, Vol. 74 Issue 5, p595-606. 12p.
Subjects: Desorption, Peptides, Blood proteins, Molecular weights, Mass spectrometry, Serum
Abstract: Abstract: This report describes a new method for desorption of low-molecular weight (LMW) peptides from abundant blood proteins for use in subsequent mass spectrometry analyses. Heating of diluted blood serum to 98°C for 15min resulted in dissociation of LMW peptides from the most abundant blood proteins. Application of blood plasma/serum fractionation using magnetic beads with a functionalized surface followed by heating of the resultant fractions significantly increases the number of LMW peptides detected by MALDI–TOF MS, enhances the general reproducibility of mass spectrometry profiles and considerably increases the number of identified blood serum peptides by LC–MS/MS using an Agilent 6520 Accurate-Mass Q-TOF. [Copyright &y& Elsevier]
Copyright of Journal of Proteomics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 59929822
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
IllustrationInfo
Items – Name: Title
  Label: Title
  Group: Ti
  Data: New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry
– Name: Author
  Label: Authors
  Group: Au
  Data: <searchLink fieldCode="AR" term="%22Ziganshin%2C+Rustam%22">Ziganshin, Rustam</searchLink><i> rustam.ziganshin@gmail.com</i><br /><searchLink fieldCode="AR" term="%22Arapidi%2C+Georgii%22">Arapidi, Georgii</searchLink><br /><searchLink fieldCode="AR" term="%22Azarkin%2C+Igor%22">Azarkin, Igor</searchLink><br /><searchLink fieldCode="AR" term="%22Zaryadieva%2C+Elena%22">Zaryadieva, Elena</searchLink><br /><searchLink fieldCode="AR" term="%22Alexeev%2C+Dmitry%22">Alexeev, Dmitry</searchLink><br /><searchLink fieldCode="AR" term="%22Govorun%2C+Vadim%22">Govorun, Vadim</searchLink><br /><searchLink fieldCode="AR" term="%22Ivanov%2C+Vadim%22">Ivanov, Vadim</searchLink>
– Name: TitleSource
  Label: Source
  Group: Src
  Data: <searchLink fieldCode="JN" term="%22Journal+of+Proteomics%22">Journal of Proteomics</searchLink>. May2011, Vol. 74 Issue 5, p595-606. 12p.
– Name: Subject
  Label: Subjects
  Group: Su
  Data: <searchLink fieldCode="DE" term="%22Desorption%22">Desorption</searchLink><br /><searchLink fieldCode="DE" term="%22Peptides%22">Peptides</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+proteins%22">Blood proteins</searchLink><br /><searchLink fieldCode="DE" term="%22Molecular+weights%22">Molecular weights</searchLink><br /><searchLink fieldCode="DE" term="%22Mass+spectrometry%22">Mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Serum%22">Serum</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: This report describes a new method for desorption of low-molecular weight (LMW) peptides from abundant blood proteins for use in subsequent mass spectrometry analyses. Heating of diluted blood serum to 98°C for 15min resulted in dissociation of LMW peptides from the most abundant blood proteins. Application of blood plasma/serum fractionation using magnetic beads with a functionalized surface followed by heating of the resultant fractions significantly increases the number of LMW peptides detected by MALDI–TOF MS, enhances the general reproducibility of mass spectrometry profiles and considerably increases the number of identified blood serum peptides by LC–MS/MS using an Agilent 6520 Accurate-Mass Q-TOF. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Proteomics is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=59929822
RecordInfo BibRecord:
  BibEntity:
    Identifiers:
      – Type: doi
        Value: 10.1016/j.jprot.2011.01.014
    Languages:
      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 12
        StartPage: 595
    Subjects:
      – SubjectFull: Desorption
        Type: general
      – SubjectFull: Peptides
        Type: general
      – SubjectFull: Blood proteins
        Type: general
      – SubjectFull: Molecular weights
        Type: general
      – SubjectFull: Mass spectrometry
        Type: general
      – SubjectFull: Serum
        Type: general
    Titles:
      – TitleFull: New method for peptide desorption from abundant blood proteins for plasma/serum peptidome analyses by mass spectrometry
        Type: main
  BibRelationships:
    HasContributorRelationships:
      – PersonEntity:
          Name:
            NameFull: Ziganshin, Rustam
      – PersonEntity:
          Name:
            NameFull: Arapidi, Georgii
      – PersonEntity:
          Name:
            NameFull: Azarkin, Igor
      – PersonEntity:
          Name:
            NameFull: Zaryadieva, Elena
      – PersonEntity:
          Name:
            NameFull: Alexeev, Dmitry
      – PersonEntity:
          Name:
            NameFull: Govorun, Vadim
      – PersonEntity:
          Name:
            NameFull: Ivanov, Vadim
    IsPartOfRelationships:
      – BibEntity:
          Dates:
            – D: 01
              M: 05
              Text: May2011
              Type: published
              Y: 2011
          Identifiers:
            – Type: issn-print
              Value: 18743919
          Numbering:
            – Type: volume
              Value: 74
            – Type: issue
              Value: 5
          Titles:
            – TitleFull: Journal of Proteomics
              Type: main
ResultId 1