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Thin layer chromatography/mass spectrometry

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Title: Thin layer chromatography/mass spectrometry
Authors: Cheng, Sy-Chyi1, Huang, Min-Zong2, Shiea, Jentaie2,3,4 jetea@fac.nsysu.edu.tw
Source: Journal of Chromatography A. May2011, Vol. 1218 Issue 19, p2700-2711. 12p.
Subjects: Thin layer chromatography, Mass spectrometry, Desorption, Statistical sampling, Ionization (Atomic physics), Ion sources, Structural plates, Biochemistry, Extraction (Chemistry)
Abstract: Abstract: Thin layer chromatography (TLC)—a simple, cost-effective, and easy-to-operate planar chromatographic technique—has been used in general chemistry laboratories for several decades to routinely separate chemical and biochemical compounds. Traditionally, chemical and optical methods are employed to visualize the analyte spots on the TLC plate. Because direct identification and structural characterization of the analytes on the TLC plate through these methods are not possible, there has been long-held interest in the development of interfaces that allow TLC to be combined with mass spectrometry (MS)—one of the most efficient analytical tools for structural elucidation. So far, many different TLC–MS techniques have been reported in the literature; some are commercially available. According to differences in their operational processes, the existing TLC–MS systems can be classified into two categories: (i) indirect mass spectrometric analyses, performed by scraping, extracting, purifying, and concentrating the analyte from the TLC plate and then directing it into the mass spectrometer''s ion source for further analysis; (ii) direct mass spectrometric analyses, where the analyte on the TLC plate is characterized directly through mass spectrometry without the need for scraping, extraction, or concentration processes. Conventionally, direct TLC–MS analysis is performed under vacuum, but the development of ambient mass spectrometry has allowed analytes on TLC plates to be characterized under atmospheric pressure. Thus, TLC–MS techniques can also be classified into two other categories according to the working environment of the ion source: vacuum-based TLC–MS or ambient TLC–MS. This review article describes the state of the art of TLC–MS techniques used for indirect and direct characterization of analytes on the surfaces of TLC plates. [Copyright &y& Elsevier]
Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
FullText Text:
  Availability: 0
Header DbId: egs
DbLabel: Engineering Source
An: 60042394
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: Thin layer chromatography/mass spectrometry
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  Data: <searchLink fieldCode="AR" term="%22Cheng%2C+Sy-Chyi%22">Cheng, Sy-Chyi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Huang%2C+Min-Zong%22">Huang, Min-Zong</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Shiea%2C+Jentaie%22">Shiea, Jentaie</searchLink><relatesTo>2,3,4</relatesTo><i> jetea@fac.nsysu.edu.tw</i>
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  Data: Abstract: Thin layer chromatography (TLC)—a simple, cost-effective, and easy-to-operate planar chromatographic technique—has been used in general chemistry laboratories for several decades to routinely separate chemical and biochemical compounds. Traditionally, chemical and optical methods are employed to visualize the analyte spots on the TLC plate. Because direct identification and structural characterization of the analytes on the TLC plate through these methods are not possible, there has been long-held interest in the development of interfaces that allow TLC to be combined with mass spectrometry (MS)—one of the most efficient analytical tools for structural elucidation. So far, many different TLC–MS techniques have been reported in the literature; some are commercially available. According to differences in their operational processes, the existing TLC–MS systems can be classified into two categories: (i) indirect mass spectrometric analyses, performed by scraping, extracting, purifying, and concentrating the analyte from the TLC plate and then directing it into the mass spectrometer''s ion source for further analysis; (ii) direct mass spectrometric analyses, where the analyte on the TLC plate is characterized directly through mass spectrometry without the need for scraping, extraction, or concentration processes. Conventionally, direct TLC–MS analysis is performed under vacuum, but the development of ambient mass spectrometry has allowed analytes on TLC plates to be characterized under atmospheric pressure. Thus, TLC–MS techniques can also be classified into two other categories according to the working environment of the ion source: vacuum-based TLC–MS or ambient TLC–MS. This review article describes the state of the art of TLC–MS techniques used for indirect and direct characterization of analytes on the surfaces of TLC plates. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography A is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.chroma.2011.01.077
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      – Code: eng
        Text: English
    PhysicalDescription:
      Pagination:
        PageCount: 12
        StartPage: 2700
    Subjects:
      – SubjectFull: Thin layer chromatography
        Type: general
      – SubjectFull: Mass spectrometry
        Type: general
      – SubjectFull: Desorption
        Type: general
      – SubjectFull: Statistical sampling
        Type: general
      – SubjectFull: Ionization (Atomic physics)
        Type: general
      – SubjectFull: Ion sources
        Type: general
      – SubjectFull: Structural plates
        Type: general
      – SubjectFull: Biochemistry
        Type: general
      – SubjectFull: Extraction (Chemistry)
        Type: general
    Titles:
      – TitleFull: Thin layer chromatography/mass spectrometry
        Type: main
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            NameFull: Cheng, Sy-Chyi
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            NameFull: Huang, Min-Zong
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            NameFull: Shiea, Jentaie
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            – D: 13
              M: 05
              Text: May2011
              Type: published
              Y: 2011
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