High yield Rhizopus chinenisis prolipase production in Pichia pastoris: Impact of methanol concentration.
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| Title: | High yield Rhizopus chinenisis prolipase production in Pichia pastoris: Impact of methanol concentration. |
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| Authors: | Wu, Dan1, Yu, Xiao2 biosean@yahoo.com.cn, Wang, Tong2, Wang, Rui2, Xu, Yan2 |
| Source: | Biotechnology & Bioprocess Engineering. Apr2011, Vol. 16 Issue 2, p305-311. 7p. |
| Abstract: | Rhizopus lipases have been successfully expressed in Pichia pastors and different fermentation strategies have been investigated. However, there is no sufficient study on the effects of methanol concentration on the production of Rhizopus lipases in P. pastors. In this study, the lipase from Rhizopus chinensis CCTCC M20102 was expressed under different fed-batch fermentation conditions at methanol concentrations ranging from 0.5 to 3.5 g/L. The lipase activity, stability, and productivities were analyzed. The optimum methanol concentration was 1 g/L, with the highest lipase activity of 2,130 U/mL, without degradation. Additional information was obtained from the analysis of methanol consumption and production rates. The results also suggested that the cell concentration at the end of the glycerol fed-batch phase was very important for cell viability and protease activity. [ABSTRACT FROM AUTHOR] |
| Copyright of Biotechnology & Bioprocess Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Items | – Name: Title Label: Title Group: Ti Data: High yield Rhizopus chinenisis prolipase production in Pichia pastoris: Impact of methanol concentration. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Wu%2C+Dan%22">Wu, Dan</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yu%2C+Xiao%22">Yu, Xiao</searchLink><relatesTo>2</relatesTo><i> biosean@yahoo.com.cn</i><br /><searchLink fieldCode="AR" term="%22Wang%2C+Tong%22">Wang, Tong</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Rui%22">Wang, Rui</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Xu%2C+Yan%22">Xu, Yan</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Biotechnology+%26+Bioprocess+Engineering%22">Biotechnology & Bioprocess Engineering</searchLink>. Apr2011, Vol. 16 Issue 2, p305-311. 7p. – Name: Abstract Label: Abstract Group: Ab Data: Rhizopus lipases have been successfully expressed in Pichia pastors and different fermentation strategies have been investigated. However, there is no sufficient study on the effects of methanol concentration on the production of Rhizopus lipases in P. pastors. In this study, the lipase from Rhizopus chinensis CCTCC M20102 was expressed under different fed-batch fermentation conditions at methanol concentrations ranging from 0.5 to 3.5 g/L. The lipase activity, stability, and productivities were analyzed. The optimum methanol concentration was 1 g/L, with the highest lipase activity of 2,130 U/mL, without degradation. Additional information was obtained from the analysis of methanol consumption and production rates. The results also suggested that the cell concentration at the end of the glycerol fed-batch phase was very important for cell viability and protease activity. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Biotechnology & Bioprocess Engineering is the property of Springer Nature and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1007/s12257-009-3021-4 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 7 StartPage: 305 Titles: – TitleFull: High yield Rhizopus chinenisis prolipase production in Pichia pastoris: Impact of methanol concentration. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Wu, Dan – PersonEntity: Name: NameFull: Yu, Xiao – PersonEntity: Name: NameFull: Wang, Tong – PersonEntity: Name: NameFull: Wang, Rui – PersonEntity: Name: NameFull: Xu, Yan IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 04 Text: Apr2011 Type: published Y: 2011 Identifiers: – Type: issn-print Value: 12268372 Numbering: – Type: volume Value: 16 – Type: issue Value: 2 Titles: – TitleFull: Biotechnology & Bioprocess Engineering Type: main |
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