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Determination of azatadine in human plasma by liquid chromatography/tandem mass spectrometry

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Title: Determination of azatadine in human plasma by liquid chromatography/tandem mass spectrometry
Authors: Zhu, Yan-rong, Jia, Yan-yan, Jiang, Ling1, Wang, Chao1, Ding, Li-kun1, Yang, Jing1, Li, Liang1, Zhao, Pei-xi1, Liu, Wen-xin1, Yi-Ding1, Wang, Li1, Wen, Ai-dong adwen-2004@hotmail.com
Source: Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. Aug2011, Vol. 879 Issue 23, p2189-2193. 5p.
Subjects: Antihistamines, Blood plasma, Liquid chromatography, Tandem mass spectrometry, Pharmacokinetics, Loratadine, Extraction (Chemistry)
Abstract: Abstract: A sensitive method using liquid chromatography with tandem mass spectrometric detection (LC–MS/MS) was developed and validated for the analysis of antihistamine drug azatadine in human plasma. Loratadine was used as internal standard (IS). Analytes were extracted from human plasma by liquid/liquid extraction using ethyl acetate. The organic phase was reduced to dryness under a stream of nitrogen at 30°C and the residue was reconstituted with the mobile phase. 5μL of the resulting solution was injected onto the LC–MS/MS system. A 4.6mm×150mm, I.D. 5μm, Agilent TC-C18 column was used to perform the chromatographic analysis. The mobile phase consisted of ammonium formate buffer 0.010M (adjusted to pH 4.3 with 1M formic acid)/acetonitrile (20:80, v/v) The chromatographic run time was 5min per injection and flow rate was 0.6mL/min. The retention time was 2.4 and 4.4min for azatadine and IS, respectively. The tandem mass spectrometric detection mode was achieved with electrospray ionization (ESI) iron source and the multiple reaction monitoring (MRM) (291.3→248.2 m/z for azatadine, 383.3→337.3 m/z for IS) was operated in positive ion modes. The low limit of quantitation (LLOQ) was 0.05ng/mL. The intra-day and inter-day precision of the quality control (QC) samples was 8.93–11.57% relative standard deviation (RSD). The inter-day accuracy of the QC samples was 96.83–105.07% of the nominal values. [Copyright &y& Elsevier]
Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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DbLabel: Engineering Source
An: 63188692
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  Data: Determination of azatadine in human plasma by liquid chromatography/tandem mass spectrometry
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  Data: <searchLink fieldCode="AR" term="%22Zhu%2C+Yan-rong%22">Zhu, Yan-rong</searchLink><br /><searchLink fieldCode="AR" term="%22Jia%2C+Yan-yan%22">Jia, Yan-yan</searchLink><br /><searchLink fieldCode="AR" term="%22Jiang%2C+Ling%22">Jiang, Ling</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Chao%22">Wang, Chao</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ding%2C+Li-kun%22">Ding, Li-kun</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yang%2C+Jing%22">Yang, Jing</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Li%2C+Liang%22">Li, Liang</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Zhao%2C+Pei-xi%22">Zhao, Pei-xi</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Liu%2C+Wen-xin%22">Liu, Wen-xin</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Yi-Ding%22">Yi-Ding</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wang%2C+Li%22">Wang, Li</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Wen%2C+Ai-dong%22">Wen, Ai-dong</searchLink><i> adwen-2004@hotmail.com</i>
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  Data: <searchLink fieldCode="JN" term="%22Journal+of+Chromatography+B%3A+Analytical+Technologies+in+the+Biomedical+%26+Life+Sciences%22">Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences</searchLink>. Aug2011, Vol. 879 Issue 23, p2189-2193. 5p.
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  Data: <searchLink fieldCode="DE" term="%22Antihistamines%22">Antihistamines</searchLink><br /><searchLink fieldCode="DE" term="%22Blood+plasma%22">Blood plasma</searchLink><br /><searchLink fieldCode="DE" term="%22Liquid+chromatography%22">Liquid chromatography</searchLink><br /><searchLink fieldCode="DE" term="%22Tandem+mass+spectrometry%22">Tandem mass spectrometry</searchLink><br /><searchLink fieldCode="DE" term="%22Pharmacokinetics%22">Pharmacokinetics</searchLink><br /><searchLink fieldCode="DE" term="%22Loratadine%22">Loratadine</searchLink><br /><searchLink fieldCode="DE" term="%22Extraction+%28Chemistry%29%22">Extraction (Chemistry)</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: Abstract: A sensitive method using liquid chromatography with tandem mass spectrometric detection (LC–MS/MS) was developed and validated for the analysis of antihistamine drug azatadine in human plasma. Loratadine was used as internal standard (IS). Analytes were extracted from human plasma by liquid/liquid extraction using ethyl acetate. The organic phase was reduced to dryness under a stream of nitrogen at 30°C and the residue was reconstituted with the mobile phase. 5μL of the resulting solution was injected onto the LC–MS/MS system. A 4.6mm×150mm, I.D. 5μm, Agilent TC-C18 column was used to perform the chromatographic analysis. The mobile phase consisted of ammonium formate buffer 0.010M (adjusted to pH 4.3 with 1M formic acid)/acetonitrile (20:80, v/v) The chromatographic run time was 5min per injection and flow rate was 0.6mL/min. The retention time was 2.4 and 4.4min for azatadine and IS, respectively. The tandem mass spectrometric detection mode was achieved with electrospray ionization (ESI) iron source and the multiple reaction monitoring (MRM) (291.3→248.2 m/z for azatadine, 383.3→337.3 m/z for IS) was operated in positive ion modes. The low limit of quantitation (LLOQ) was 0.05ng/mL. The intra-day and inter-day precision of the quality control (QC) samples was 8.93–11.57% relative standard deviation (RSD). The inter-day accuracy of the QC samples was 96.83–105.07% of the nominal values. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
  Group: Ab
  Data: <i>Copyright of Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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RecordInfo BibRecord:
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      – Type: doi
        Value: 10.1016/j.jchromb.2011.05.050
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      – Code: eng
        Text: English
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      Pagination:
        PageCount: 5
        StartPage: 2189
    Subjects:
      – SubjectFull: Antihistamines
        Type: general
      – SubjectFull: Blood plasma
        Type: general
      – SubjectFull: Liquid chromatography
        Type: general
      – SubjectFull: Tandem mass spectrometry
        Type: general
      – SubjectFull: Pharmacokinetics
        Type: general
      – SubjectFull: Loratadine
        Type: general
      – SubjectFull: Extraction (Chemistry)
        Type: general
    Titles:
      – TitleFull: Determination of azatadine in human plasma by liquid chromatography/tandem mass spectrometry
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            NameFull: Zhu, Yan-rong
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              M: 08
              Text: Aug2011
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              Y: 2011
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