Response of choline metabolites to docetaxel therapy is quantified in vivo by localized 31P MRS of human breast cancer xenografts and in vitro by high-resolution 31P NMR spectroscopy of cell extracts.
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| Title: | Response of choline metabolites to docetaxel therapy is quantified in vivo by localized 31P MRS of human breast cancer xenografts and in vitro by high-resolution 31P NMR spectroscopy of cell extracts. |
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| Authors: | Morse, David L.1, Raghunand, Natarajan2, Sadarangani, Pooja2, Murthi, Shiva2, Job, Constantin3, Day, Sam2, Howison, Christine3, Gillies, Robert J.2 |
| Source: | Magnetic Resonance in Medicine. Aug2007, Vol. 58 Issue 2, p270-280. 11p. |
| Abstract: | Choline-containing compounds (CCCs) are elevated in breast cancer, and detected in vivo by the 1H MRS total choline (tCho) resonance (3.25 ppm) and the 31P MRS phosphomonoester (PME) resonance (3.8 ppm). Both the tCho and PME resonances decrease early after initiation of successful therapy. The single major component of these composite resonances, phosphocholine (PCho), also responds to therapy by decreasing. The ability to resolve and quantify PCho in vivo would thus increase the sensitivity of this biomarker for early detection of therapeutic response.Herein, the in vivo resolution and quantification of PCho is reported in human mouse xenograft tumors of the human breast cancer cell lines MCF-7 and MDA-mb-231. Significant decreases in tumor PCho are observed within 2 to 4 d posttreatment with the antimicrotubule drug, docetaxel. To determine whether these decreases are a general tumor response or an intracellular metabolic response, high-resolution NMR spectroscopy was performed on extracts of cells treated with docetaxel. Significant decreases in intracellular PCho and increases in glycerophosphocholine (GPC) were observed. These decreases are coincident with other tumor and cellular responses such as tumor growth delay (TGD), cell-cycle arrest, and modes of cell death such as mitotic catastrophe, necrosis, and apoptosis, with mitotic catastrophe predominating. Magn Reson Med 58:270-280, 2007. © 2007 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] |
| Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
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| Header | DbId: egs DbLabel: Engineering Source An: 63336390 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: Response of choline metabolites to docetaxel therapy is quantified in vivo by localized <superscript>31</superscript>P MRS of human breast cancer xenografts and in vitro by high-resolution <superscript>31</superscript>P NMR spectroscopy of cell extracts. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Morse%2C+David+L%2E%22">Morse, David L.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Raghunand%2C+Natarajan%22">Raghunand, Natarajan</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Sadarangani%2C+Pooja%22">Sadarangani, Pooja</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Murthi%2C+Shiva%22">Murthi, Shiva</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Job%2C+Constantin%22">Job, Constantin</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Day%2C+Sam%22">Day, Sam</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Howison%2C+Christine%22">Howison, Christine</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Gillies%2C+Robert+J%2E%22">Gillies, Robert J.</searchLink><relatesTo>2</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Magnetic+Resonance+in+Medicine%22">Magnetic Resonance in Medicine</searchLink>. Aug2007, Vol. 58 Issue 2, p270-280. 11p. – Name: Abstract Label: Abstract Group: Ab Data: Choline-containing compounds (CCCs) are elevated in breast cancer, and detected in vivo by the 1H MRS total choline (tCho) resonance (3.25 ppm) and the 31P MRS phosphomonoester (PME) resonance (3.8 ppm). Both the tCho and PME resonances decrease early after initiation of successful therapy. The single major component of these composite resonances, phosphocholine (PCho), also responds to therapy by decreasing. The ability to resolve and quantify PCho in vivo would thus increase the sensitivity of this biomarker for early detection of therapeutic response.Herein, the in vivo resolution and quantification of PCho is reported in human mouse xenograft tumors of the human breast cancer cell lines MCF-7 and MDA-mb-231. Significant decreases in tumor PCho are observed within 2 to 4 d posttreatment with the antimicrotubule drug, docetaxel. To determine whether these decreases are a general tumor response or an intracellular metabolic response, high-resolution NMR spectroscopy was performed on extracts of cells treated with docetaxel. Significant decreases in intracellular PCho and increases in glycerophosphocholine (GPC) were observed. These decreases are coincident with other tumor and cellular responses such as tumor growth delay (TGD), cell-cycle arrest, and modes of cell death such as mitotic catastrophe, necrosis, and apoptosis, with mitotic catastrophe predominating. Magn Reson Med 58:270-280, 2007. © 2007 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/mrm.21333 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 11 StartPage: 270 Titles: – TitleFull: Response of choline metabolites to docetaxel therapy is quantified in vivo by localized 31P MRS of human breast cancer xenografts and in vitro by high-resolution 31P NMR spectroscopy of cell extracts. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Morse, David L. – PersonEntity: Name: NameFull: Raghunand, Natarajan – PersonEntity: Name: NameFull: Sadarangani, Pooja – PersonEntity: Name: NameFull: Murthi, Shiva – PersonEntity: Name: NameFull: Job, Constantin – PersonEntity: Name: NameFull: Day, Sam – PersonEntity: Name: NameFull: Howison, Christine – PersonEntity: Name: NameFull: Gillies, Robert J. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2007 Type: published Y: 2007 Identifiers: – Type: issn-print Value: 07403194 Numbering: – Type: volume Value: 58 – Type: issue Value: 2 Titles: – TitleFull: Magnetic Resonance in Medicine Type: main |
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