13C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized 13C-labeled glutamine.
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| Title: | 13C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized 13C-labeled glutamine. |
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| Authors: | Gallagher, Ferdia A.1,2, Kettunen, Mikko I.1, Day, Sam E.1, Lerche, Mathilde3, Brindle, Kevin M.1 |
| Source: | Magnetic Resonance in Medicine. Aug2008, Vol. 60 Issue 2, p253-257. 5p. |
| Abstract: | Dynamic nuclear polarization (DNP) is an emerging technique for increasing the sensitivity of 13C MR spectroscopy (MRS). [5-13C1]Glutamine was hyperpolarized using this technique by up to 5%, representing a 6000-fold increase in sensitivity. The conversion of hyperpolarized glutamine to glutamate by mitochondrial glutaminase was demonstrated using 13C-MRS measurements in cultured human hepatoma cells (HepG2). These results represent the first step in developing an imaging technique for detecting glutamine metabolism in vivo. Furthermore, since glutamine utilization has been correlated with cell proliferation, the study suggests a new technique for detecting changes in tumor cell proliferation. Magn Reson Med 60:253-257, 2008. © 2008 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] |
| Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.) | |
| Database: | Engineering Source |
| FullText | Text: Availability: 0 |
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| Header | DbId: egs DbLabel: Engineering Source An: 63336741 AccessLevel: 6 PubType: Academic Journal PubTypeId: academicJournal PreciseRelevancyScore: 0 |
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| Items | – Name: Title Label: Title Group: Ti Data: <superscript>13</superscript>C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized <superscript>13</superscript>C-labeled glutamine. – Name: Author Label: Authors Group: Au Data: <searchLink fieldCode="AR" term="%22Gallagher%2C+Ferdia+A%2E%22">Gallagher, Ferdia A.</searchLink><relatesTo>1,2</relatesTo><br /><searchLink fieldCode="AR" term="%22Kettunen%2C+Mikko+I%2E%22">Kettunen, Mikko I.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Day%2C+Sam+E%2E%22">Day, Sam E.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Lerche%2C+Mathilde%22">Lerche, Mathilde</searchLink><relatesTo>3</relatesTo><br /><searchLink fieldCode="AR" term="%22Brindle%2C+Kevin+M%2E%22">Brindle, Kevin M.</searchLink><relatesTo>1</relatesTo> – Name: TitleSource Label: Source Group: Src Data: <searchLink fieldCode="JN" term="%22Magnetic+Resonance+in+Medicine%22">Magnetic Resonance in Medicine</searchLink>. Aug2008, Vol. 60 Issue 2, p253-257. 5p. – Name: Abstract Label: Abstract Group: Ab Data: Dynamic nuclear polarization (DNP) is an emerging technique for increasing the sensitivity of 13C MR spectroscopy (MRS). [5-13C1]Glutamine was hyperpolarized using this technique by up to 5%, representing a 6000-fold increase in sensitivity. The conversion of hyperpolarized glutamine to glutamate by mitochondrial glutaminase was demonstrated using 13C-MRS measurements in cultured human hepatoma cells (HepG2). These results represent the first step in developing an imaging technique for detecting glutamine metabolism in vivo. Furthermore, since glutamine utilization has been correlated with cell proliferation, the study suggests a new technique for detecting changes in tumor cell proliferation. Magn Reson Med 60:253-257, 2008. © 2008 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR] – Name: AbstractSuppliedCopyright Label: Group: Ab Data: <i>Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.) |
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| RecordInfo | BibRecord: BibEntity: Identifiers: – Type: doi Value: 10.1002/mrm.21650 Languages: – Code: eng Text: English PhysicalDescription: Pagination: PageCount: 5 StartPage: 253 Titles: – TitleFull: 13C MR spectroscopy measurements of glutaminase activity in human hepatocellular carcinoma cells using hyperpolarized 13C-labeled glutamine. Type: main BibRelationships: HasContributorRelationships: – PersonEntity: Name: NameFull: Gallagher, Ferdia A. – PersonEntity: Name: NameFull: Kettunen, Mikko I. – PersonEntity: Name: NameFull: Day, Sam E. – PersonEntity: Name: NameFull: Lerche, Mathilde – PersonEntity: Name: NameFull: Brindle, Kevin M. IsPartOfRelationships: – BibEntity: Dates: – D: 01 M: 08 Text: Aug2008 Type: published Y: 2008 Identifiers: – Type: issn-print Value: 07403194 Numbering: – Type: volume Value: 60 – Type: issue Value: 2 Titles: – TitleFull: Magnetic Resonance in Medicine Type: main |
| ResultId | 1 |
