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Magnetization transfer measurements of exchange between hyperpolarized [1-13C]pyruvate and [1-13C]lactate in a murine lymphoma.

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Title: Magnetization transfer measurements of exchange between hyperpolarized [1-13C]pyruvate and [1-13C]lactate in a murine lymphoma.
Authors: Kettunen, Mikko I.1,2, Hu, De-en1,2, Witney, Timothy H.1,2, McLaughlin, Rebekah1,2, Gallagher, Ferdia A.1,2,3, Bohndiek, Sarah E.1,2, Day, Sam E.1,2, Brindle, Kevin M.1,2
Source: Magnetic Resonance in Medicine. Apr2010, Vol. 63 Issue 4, p872-880. 9p.
Abstract: Measurements of the conversion of hyperpolarized [1-13C]pyruvate into lactate, in the reaction catalyzed by lactate dehydrogenase, have shown promise as a metabolic marker for the presence of disease and response to treatment. However, it is unclear whether this represents net flux of label from pyruvate to lactate or exchange of isotope between metabolites that are close to chemical equilibrium. Using saturation and inversion transfer experiments, we show that there is significant exchange of label between lactate and pyruvate in a murine lymphoma in vivo. The rate constants estimated from the magnetization transfer experiments, at specific points during the time course of label exchange, were similar to those obtained by fitting the changes in peak intensities during the entire exchange time course to a kinetic model for two-site exchange. These magnetization transfer experiments may therefore provide an alternative and more rapid way of estimating flux between pyruvate and lactate to serial measurements of pyruvate and lactate 13C peak intensities following injection of hyperpolarized [1-13C]pyruvate. Magn Reson Med 63:872-880, 2010. © 2010 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]
Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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  Data: Magnetization transfer measurements of exchange between hyperpolarized [1-<superscript>13</superscript>C]pyruvate and [1-<superscript>13</superscript>C]lactate in a murine lymphoma.
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  Data: <searchLink fieldCode="JN" term="%22Magnetic+Resonance+in+Medicine%22">Magnetic Resonance in Medicine</searchLink>. Apr2010, Vol. 63 Issue 4, p872-880. 9p.
– Name: Abstract
  Label: Abstract
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  Data: Measurements of the conversion of hyperpolarized [1-13C]pyruvate into lactate, in the reaction catalyzed by lactate dehydrogenase, have shown promise as a metabolic marker for the presence of disease and response to treatment. However, it is unclear whether this represents net flux of label from pyruvate to lactate or exchange of isotope between metabolites that are close to chemical equilibrium. Using saturation and inversion transfer experiments, we show that there is significant exchange of label between lactate and pyruvate in a murine lymphoma in vivo. The rate constants estimated from the magnetization transfer experiments, at specific points during the time course of label exchange, were similar to those obtained by fitting the changes in peak intensities during the entire exchange time course to a kinetic model for two-site exchange. These magnetization transfer experiments may therefore provide an alternative and more rapid way of estimating flux between pyruvate and lactate to serial measurements of pyruvate and lactate 13C peak intensities following injection of hyperpolarized [1-13C]pyruvate. Magn Reson Med 63:872-880, 2010. © 2010 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]
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  Data: <i>Copyright of Magnetic Resonance in Medicine is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
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