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The development of collagen-GAG scaffold-membrane composites for tendon tissue engineering

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Title: The development of collagen-GAG scaffold-membrane composites for tendon tissue engineering
Authors: Caliari, Steven R.1, Ramirez, Manuel A.2, Harley, Brendan A.C.1,3 bharley@illinois.edu
Source: Biomaterials. Dec2011, Vol. 32 Issue 34, p8990-8998. 9p.
Subjects: Tissue engineering, Tissue scaffolds, Collagen, Glycosaminoglycans, Tendons, Cell proliferation, Biomedical materials, Mechanical behavior of materials
Abstract: Abstract: Current tissue engineering approaches for tendon defects require improved biomaterials to balance microstructural and mechanical design criteria. Collagen-glycosaminoglycan (CG) scaffolds have shown considerable success as in vivo regenerative templates and in vitro constructs to study cell behavior. While these scaffolds possess many advantageous qualities, their mechanical properties are typically orders of magnitude lower than orthopedic tissues such as tendon. Taking inspiration from mechanically efficient core–shell composites in nature such as plant stems and porcupine quills, we have created core–shell CG composites that display high bioactivity and improved mechanical integrity. These composites feature integration of a low density, anisotropic CG scaffold core with a high density, CG membrane shell. CG membranes were fabricated via an evaporative process that allowed separate tuning of membrane thickness and elastic moduli and were found to be isotropic in-plane. The membranes were then integrated with an anisotropic CG scaffold core via freeze-drying and subsequent crosslinking. Increasing the relative thickness of the CG membrane shell was shown to increase composite tensile elastic modulus by as much as a factor of 36 in a manner consistent with predictions from layered composites theory. CG scaffold-membrane composites were found to support tendon cell viability, proliferation, and metabolic activity in vitro, suggesting they maintain sufficient permeability while demonstrating improved mechanical strength. This work suggests an effective, biomimetic approach for balancing strength and bioactivity requirements of porous scaffolds for tissue engineering. [Copyright &y& Elsevier]
Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Database: Engineering Source
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Header DbId: egs
DbLabel: Engineering Source
An: 65944484
AccessLevel: 6
PubType: Academic Journal
PubTypeId: academicJournal
PreciseRelevancyScore: 0
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  Data: The development of collagen-GAG scaffold-membrane composites for tendon tissue engineering
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  Data: <searchLink fieldCode="AR" term="%22Caliari%2C+Steven+R%2E%22">Caliari, Steven R.</searchLink><relatesTo>1</relatesTo><br /><searchLink fieldCode="AR" term="%22Ramirez%2C+Manuel+A%2E%22">Ramirez, Manuel A.</searchLink><relatesTo>2</relatesTo><br /><searchLink fieldCode="AR" term="%22Harley%2C+Brendan+A%2EC%2E%22">Harley, Brendan A.C.</searchLink><relatesTo>1,3</relatesTo><i> bharley@illinois.edu</i>
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  Data: <searchLink fieldCode="JN" term="%22Biomaterials%22">Biomaterials</searchLink>. Dec2011, Vol. 32 Issue 34, p8990-8998. 9p.
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  Data: <searchLink fieldCode="DE" term="%22Tissue+engineering%22">Tissue engineering</searchLink><br /><searchLink fieldCode="DE" term="%22Tissue+scaffolds%22">Tissue scaffolds</searchLink><br /><searchLink fieldCode="DE" term="%22Collagen%22">Collagen</searchLink><br /><searchLink fieldCode="DE" term="%22Glycosaminoglycans%22">Glycosaminoglycans</searchLink><br /><searchLink fieldCode="DE" term="%22Tendons%22">Tendons</searchLink><br /><searchLink fieldCode="DE" term="%22Cell+proliferation%22">Cell proliferation</searchLink><br /><searchLink fieldCode="DE" term="%22Biomedical+materials%22">Biomedical materials</searchLink><br /><searchLink fieldCode="DE" term="%22Mechanical+behavior+of+materials%22">Mechanical behavior of materials</searchLink>
– Name: Abstract
  Label: Abstract
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  Data: Abstract: Current tissue engineering approaches for tendon defects require improved biomaterials to balance microstructural and mechanical design criteria. Collagen-glycosaminoglycan (CG) scaffolds have shown considerable success as in vivo regenerative templates and in vitro constructs to study cell behavior. While these scaffolds possess many advantageous qualities, their mechanical properties are typically orders of magnitude lower than orthopedic tissues such as tendon. Taking inspiration from mechanically efficient core–shell composites in nature such as plant stems and porcupine quills, we have created core–shell CG composites that display high bioactivity and improved mechanical integrity. These composites feature integration of a low density, anisotropic CG scaffold core with a high density, CG membrane shell. CG membranes were fabricated via an evaporative process that allowed separate tuning of membrane thickness and elastic moduli and were found to be isotropic in-plane. The membranes were then integrated with an anisotropic CG scaffold core via freeze-drying and subsequent crosslinking. Increasing the relative thickness of the CG membrane shell was shown to increase composite tensile elastic modulus by as much as a factor of 36 in a manner consistent with predictions from layered composites theory. CG scaffold-membrane composites were found to support tendon cell viability, proliferation, and metabolic activity in vitro, suggesting they maintain sufficient permeability while demonstrating improved mechanical strength. This work suggests an effective, biomimetic approach for balancing strength and bioactivity requirements of porous scaffolds for tissue engineering. [Copyright &y& Elsevier]
– Name: AbstractSuppliedCopyright
  Label:
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  Data: <i>Copyright of Biomaterials is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract.</i> (Copyright applies to all Abstracts.)
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=egs&AN=65944484
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        Value: 10.1016/j.biomaterials.2011.08.035
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        Text: English
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      – SubjectFull: Collagen
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      – SubjectFull: Glycosaminoglycans
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      – SubjectFull: Tendons
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      – SubjectFull: Cell proliferation
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      – SubjectFull: Biomedical materials
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      – SubjectFull: Mechanical behavior of materials
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      – TitleFull: The development of collagen-GAG scaffold-membrane composites for tendon tissue engineering
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            NameFull: Ramirez, Manuel A.
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              Text: Dec2011
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              Y: 2011
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